Volume 5, Issue 1 (3-2023)                   Plant Biotechnol Persa 2023, 5(1): 0-0 | Back to browse issues page

XML Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Hosseini S M, Yousofvand N. Application survey of zinc nanoparticles containing Ocimum basilicum leaf aqueous extract on nicotine-induced cell death in PC12: A pre-clinical trial study. Plant Biotechnol Persa 2023; 5 (1)
URL: http://pbp.medilam.ac.ir/article-1-167-en.html
Department of Biology, Faculty of Science, Razi University, 67149-67346, Kermanshah, Iran , yousofnam@yahoo.com
Abstract:   (22 Views)
High concentration of nicotine induces cell death in central nervous system which can affect the health of nicotine-exposed people. Some therapeutic properties of Ocimum basilicum leaves and zinc nanoparticles have been investigated but its protective power against neuron cell death not studied yet. Therefore, we studied the protective effect of zinc nanoparticles green-synthesized by Ocimum basilicum leaves aqueous extract in nicotine-treated PC12 cells. The cell viability measured by LDH test. TUNEL test was used to show DNA fragmentation and apoptosis. The Rat inflammatory cytokine assay kit was used to measurement of inflammatory cytokines concentrations. 100 mM of nicotine reduced cell viability, and increased cell cytotoxicity, inflammatory cytokines concentrations, and DNA fragmentation significantly. Zinc nanoparticles green-synthesized by Ocimum basilicum leaves aqueous extract increased cell viability and decreased cell cytotoxicity, inflammatory cytokines concentrations, and DNA fragmentation in high concentration nicotine-treated in PC12 cells, respectively. The main finding of our study was that zinc nanoparticles green-synthesized by Ocimum basilicum leaves aqueous extract suppresses the nicotine-induced cell death in PC12 neuron-like cells. It inactivates the apoptosis process through inflammatory cytokines synthesis.

 
     
Type of Study: Research | Subject: Herbal Drugs
Received: 2022/11/22 | Accepted: 2022/11/23 | Published: 2022/11/23

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.