en Green Chemistry پژوهشي Research <span style="font-size:14px;"><span style="font-family:Times New Roman;"><span style="line-height:normal"><b><span new="" roman="" times="">Objective:</span></b> <span lang="EN" new="" roman="" times="">In nanotechnology, nanoparticles (NPs) are nowadays recognized as a beneficial tool for dental treatments. Metal NPs, especially copper oxide (CuO) NPs, have the potential for preventing and treating bacterial dental infections. This study was designed to assess the antimicrobial efficacy of CuO NPs synthesized using the aqueous extract of </span><em>Cotoneaster</em><b> </b><span new="" roman="" times="">plant<b> </b>against<b> </b></span><span lang="EN" new="" roman="" times="">biofilm-producing <i>Enterococcus faecalis</i>.</span></span><br> <span style="line-height:normal"><b><span lang="EN" new="" roman="" times="">Methods:</span></b><span lang="EN" new="" roman="" times=""> For this purpose, we used the extract of the aforesaid plant and a green method to synthesize CuO NPs. The NPs synthesized herein were assessed using FTIR, XRD, UV-Vis, and SEM. Likewise, their antimicrobial and antibiofilm effects against <i>E. faecalis</i> were examined using the microdilution method. <b>Results:</b> NPs were found to have sizes of 20-100 nm, in addition to having a monoclinic crystal structure. The synthesized NPs and chlorhexidine displayed minimum inhibitory concentrations of 1.25 &mu;g/mL and 31.25 &mu;g/mL, respectively. In antibiofilm testing, the NPs led to a relative reduction in biofilm formation and bacterial growth. </span></span><br> <span style="line-height:normal"><b><span lang="EN" new="" roman="" times="">Conclusion:</span></b><span lang="EN" new="" roman="" times=""> Overall, our findings suggest that green-synthesized CuO NPs derived from </span><em>Cotoneaster</em><b> </b><span lang="EN" new="" roman="" times="">extract could be a suitable option for controlling microbial infections due to their strong antimicrobial and antibiofilm effects.</span></span><br> <img alt="" src="./files/site1/images/copuediting.png" style="width: 586px; height: 265px;" ><br> &nbsp;</span></span> ,Enterococcus faecalis,metal nanoparticles,anti-biofilm properties,Antibacterial properties,green-synthesized nanoparticles 0 0 http://pbp.medilam.ac.ir/browse.php?a_code=A-10-82-3&slc_lang=en&sid=1 Asma Sepahdar asma.sepahdar@gmail.com 10031947532846006692 0009-0001-8235-3066 No Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran2School of Dentistry, Lorestan University of Medical Sciences, Khorramabad, Iran Farhad Estabraghi Farhad.Estabraghi@yahoo.com 10031947532846006693 10031947532846006693 No School of Dentistry, Lorestan University of Medical Sciences, Khorramabad, Iran abdolrazagh marzban marzban86@gmail.com 10031947532846006694 10031947532846006694 No Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran Maryam Karkhane Maryam.Karkhane@yahoo.com 10031947532846006695 10031947532846006695 No Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran Mohammad Mehrnia Mohammad.Mehrnia@yahoo.com 10031947532846006696 10031947532846006696 No Lorestan Agricultural and Natural Resources Research and Education Center, Agricultural Research Education and Extention Organization, Khorramabad, Iran pegah shakib shakib.pegah@yahoo.com 10031947532846006697 10031947532846006697 Yes Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran Mahmoud Bahmani mahmood.bahmani@ymail.com 10031947532846006698 10031947532846006698 No Biotechnology and Medicinal Plants Research Center, Ilam University of Medical Sciences, Ilam, Iran