Volume 4, Issue 2 (12-2022)
pbp 2022, 4(2): 75-87 |
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1- Department of Biochemistry, Sokoto State University, PMB 2134, Sokoto State, Nigeria , mustyeeser029@gmail.com
2- Department of Biochemistry, Sokoto State University, PMB 2134, Sokoto State, Nigeria
3- epartment of Microbiology, Sokoto State University, Sokoto State, Nigeria
2- Department of Biochemistry, Sokoto State University, PMB 2134, Sokoto State, Nigeria
3- epartment of Microbiology, Sokoto State University, Sokoto State, Nigeria
Abstract: (674 Views)
Objective: The objective of this research was focused on the development of new organoclay based composite that serves as both antibacterial and dye removing agent for the treatment of microbial and dyes contaminated water from the source.
Material and Methods: The cation exchange capacity (CEC) of the kaolinite was improved through acid treatment. Chlorhexidine- loaded zinc- kaolinite was prepared via adsorption of chlorhexidine acetate (0.5 mmol/L) on zinc-kaolinite. The composites were characterized using Field Emission Scanning Electron Microscopy (FESEM) and Energy Dispersive X-ray (EDX). The antibacterial assays of the composites were conducted against Staphylococcus aureus (S. aureus) and Salmonella typhi (S. typhi) using disc diffusion technique (DDT), minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC).
Results: The CEC value of the acid treated kaolinite (Kaot2) was improved from 9.26 + 0.82 to 13.43+1.61 meq/100g, the morphology of the composite remains intact and indicate the presence of Zinc (Zn) after formulation. The target composite (Chx-Zn-Kaot2) shows its effectiveness against S. aureus and S. typhi showing the inhibition zones of 26 mm and 1.5 mm respectively. Similarly, MIC, with 120 mg/mL inhibit both organisms while MBC revealed that the target composite, 60 mg/mL kills S. aureus and 120 mg/mL kills S. typhi respectively.
Conclusion: The formulated target composite is a good candidate for the treatment of drinking water contaminated with such microorganisms and can be able to remove substantial content of dyes.
Material and Methods: The cation exchange capacity (CEC) of the kaolinite was improved through acid treatment. Chlorhexidine- loaded zinc- kaolinite was prepared via adsorption of chlorhexidine acetate (0.5 mmol/L) on zinc-kaolinite. The composites were characterized using Field Emission Scanning Electron Microscopy (FESEM) and Energy Dispersive X-ray (EDX). The antibacterial assays of the composites were conducted against Staphylococcus aureus (S. aureus) and Salmonella typhi (S. typhi) using disc diffusion technique (DDT), minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC).
Results: The CEC value of the acid treated kaolinite (Kaot2) was improved from 9.26 + 0.82 to 13.43+1.61 meq/100g, the morphology of the composite remains intact and indicate the presence of Zinc (Zn) after formulation. The target composite (Chx-Zn-Kaot2) shows its effectiveness against S. aureus and S. typhi showing the inhibition zones of 26 mm and 1.5 mm respectively. Similarly, MIC, with 120 mg/mL inhibit both organisms while MBC revealed that the target composite, 60 mg/mL kills S. aureus and 120 mg/mL kills S. typhi respectively.
Conclusion: The formulated target composite is a good candidate for the treatment of drinking water contaminated with such microorganisms and can be able to remove substantial content of dyes.
Received: 2022/11/30 | Accepted: 2022/12/1 | Published: 2022/12/1
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