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Isah M. Evaluating Antagonistic Inhibitory Effect of Some Antibiotic–Silver Nanoparticle Combinations. pbp 2026; 8 (1)
URL: http://pbp.medilam.ac.ir/article-1-341-en.html
URL: http://pbp.medilam.ac.ir/article-1-341-en.html
1. Department of Biosciences, Faculty of Science, Universiti Teknologi Malaysia, 81310 UTM Skudai, Johor, Malaysia2. Department of Biochemistry and Molecular Biology, Sokoto State University, Birnin Kebbi Rd, Sokoto 852101, Sokoto, Nigeria , imustapha@graduate.utm.my
Abstract: (85 Views)
Background: This study reports on the green synthesis of silver nanoparticles (AgNPs) using Moringa oleifera leaf extract as a natural reducing and stabilizing agent. The synthesized AgNPs were comprehensively characterized by UV–visible spectroscopy, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), field emission scanning electron microscopy (FESEM), energy dispersive X-ray spectroscopy (EDX), transmission electron microscopy (TEM), and zeta potential (ZP) analysis.
Method: AgNPs was synthesized through green approach using M. oleifera leaves extract as the source of reducing and stabilizing agent followed by its combination with some selected antibiotics and assess their inhibition modes.
Objective: To determine the antibacterial mode of inhibition of AgNPs upon combination with antibiotics.
Result: The characterization confirmed the formation of uniformly distributed, spherical AgNPs with an average diameter of ~20 nm and a negative surface charge of –27.1 mV, indicating a high colloidal stability. To investigate their biomedical relevance, AgNPs were combined with conventional antibiotics via an imbibition method and tested against Staphylococcus aureus (S. aureus), methicillin-resistant S. aureus (MRSA), Escherichia coli (E. coli), and Pseudomonas aeruginosa (P. aeruginosa) using the Disc diffusion test (DDT).
Conclusion: The results showed different antibacterial responses, with certain AgNPs–antibiotic combinations exhibiting synergistic enhancement of inhibition zones, while others showed antagonistic interactions. These results highlight the potential of M. oleifera-mediated AgNPs as an environmentally friendly nanomaterial to enhance antibiotic activity but also emphasize the need for critical evaluation of nanoparticle–drug interactions prior to clinical application.
Method: AgNPs was synthesized through green approach using M. oleifera leaves extract as the source of reducing and stabilizing agent followed by its combination with some selected antibiotics and assess their inhibition modes.
Objective: To determine the antibacterial mode of inhibition of AgNPs upon combination with antibiotics.
Result: The characterization confirmed the formation of uniformly distributed, spherical AgNPs with an average diameter of ~20 nm and a negative surface charge of –27.1 mV, indicating a high colloidal stability. To investigate their biomedical relevance, AgNPs were combined with conventional antibiotics via an imbibition method and tested against Staphylococcus aureus (S. aureus), methicillin-resistant S. aureus (MRSA), Escherichia coli (E. coli), and Pseudomonas aeruginosa (P. aeruginosa) using the Disc diffusion test (DDT).
Conclusion: The results showed different antibacterial responses, with certain AgNPs–antibiotic combinations exhibiting synergistic enhancement of inhibition zones, while others showed antagonistic interactions. These results highlight the potential of M. oleifera-mediated AgNPs as an environmentally friendly nanomaterial to enhance antibiotic activity but also emphasize the need for critical evaluation of nanoparticle–drug interactions prior to clinical application.
Type of Study: Research |
Subject:
Green Chemistry
Received: 2025/08/25 | Accepted: 2025/09/4 | Published: 2025/12/1
Received: 2025/08/25 | Accepted: 2025/09/4 | Published: 2025/12/1
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