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Mohammadi S, Taheri A. Exploring the In Vitro Antioxidant Activity of Organic Extracts from the Red Alga Laurencia obtusa Found Along the Chabahar Coast. pbp 2026; 8
URL: http://pbp.medilam.ac.ir/article-1-388-en.html
URL: http://pbp.medilam.ac.ir/article-1-388-en.html
1- CMU
2- Marine Sci. Faculty, Chabahar Maritime University ,taherienator@gmail.com
2- Marine Sci. Faculty, Chabahar Maritime University ,
Abstract: (16 Views)
Objective: The discovery of antioxidant substances with great nutraceutical value and minor side effects for applications in preventive medicine, pharmaceuticals, and the food industry is a rapidly growing area of research. Consequently, algae, particularly marine macroalgae, are increasingly recognized as valuable sources of bioactive compounds with properties such as antioxidant, antitumor, and antihypertensive effects. This makes them ideal for use in the food and pharmaceutical industries, and they have attracted significant research interest. The algae along the Chabahar coast are rich in potential for future research. They are a potential resource for several studies. This study investigated the antioxidant characteristics of the red alga Laurencia obtusa collected from Chabahar Bay in vitro.
Methods: Algae were collected from the intertidal zone of Chabahar at a certain depth during the peak growth season. The process of using seawater and then fresh water for washing was followed by drying and grinding the algae. The extraction process was conducted using a maceration technique with an algae-to-solvent ratio of 1:10 over a 24-hour period. The solvents used were methanol, hexane, dichloromethane, and chloroform, each at concentrations of 0.01, 0.1, and 1 mg/mL. Antioxidant activity was assessed in vitro through DPPH free radical scavenging, ferrous ion chelating activity, and reducing power assays, with each assay performed in triplicates. In the DPPH and reducing power assays, ascorbic acid served as the positive control, while EDTA was used as the positive control in the chelating assay. Statistical analysis was conducted using one-way analysis of variance (ANOVA), followed by Tukey's multiple comparison test. Data processing was performed using GraphPad Prism 7 software, and the graphs and determination of IC₅₀ were determined in Excel by applying linear regression of the different concentrations against the responses to obtain the linear formula.
Results: The assays indicated that the chloroform extract (1.38 mg/mL) exhibited the lowest IC₅₀ value and the highest DPPH free radical scavenging activity for L. obtusa. The scavenging activity of the chloroform extract was significantly greater (p < 0.0001) at all tested concentrations than that of the hexane and dichloromethane extracts. Conversely, the methanol extract exhibited the lowest radical-scavenging activity (p < 0.05). In the chelating assay, the IC₅₀ value for chloroform (1.95 mg/mL) was higher than that for hexane (2.61 mg/mL), dichloromethane (4.86 mg/mL), and methanol (5.44 mg/mL) (p < 0.05). The highest reducing power was observed in the hexane extract, followed in decreasing order by the chloroform, methanol, and dichloromethane extracts. It was concluded that the increase in concentration of the active substance increased the antioxidant activity of the extracts under study, with significant differences noted at 1 mg/mL compared to other concentrations in the different treatments (p < 0.05).
Conclusion: Low-polarity organic solvents are better solvents than high-polarity solvents for extracting antioxidants from L. obtusa, as they yield extracts with the highest in vitro activity. The extracts contain antioxidant compounds with a high electron-donating capacity and metal ion-chelating power. It is advisable to identify and purify the antioxidant compounds found in the chloroform and hexane extracts of L. obtusa.
Methods: Algae were collected from the intertidal zone of Chabahar at a certain depth during the peak growth season. The process of using seawater and then fresh water for washing was followed by drying and grinding the algae. The extraction process was conducted using a maceration technique with an algae-to-solvent ratio of 1:10 over a 24-hour period. The solvents used were methanol, hexane, dichloromethane, and chloroform, each at concentrations of 0.01, 0.1, and 1 mg/mL. Antioxidant activity was assessed in vitro through DPPH free radical scavenging, ferrous ion chelating activity, and reducing power assays, with each assay performed in triplicates. In the DPPH and reducing power assays, ascorbic acid served as the positive control, while EDTA was used as the positive control in the chelating assay. Statistical analysis was conducted using one-way analysis of variance (ANOVA), followed by Tukey's multiple comparison test. Data processing was performed using GraphPad Prism 7 software, and the graphs and determination of IC₅₀ were determined in Excel by applying linear regression of the different concentrations against the responses to obtain the linear formula.
Results: The assays indicated that the chloroform extract (1.38 mg/mL) exhibited the lowest IC₅₀ value and the highest DPPH free radical scavenging activity for L. obtusa. The scavenging activity of the chloroform extract was significantly greater (p < 0.0001) at all tested concentrations than that of the hexane and dichloromethane extracts. Conversely, the methanol extract exhibited the lowest radical-scavenging activity (p < 0.05). In the chelating assay, the IC₅₀ value for chloroform (1.95 mg/mL) was higher than that for hexane (2.61 mg/mL), dichloromethane (4.86 mg/mL), and methanol (5.44 mg/mL) (p < 0.05). The highest reducing power was observed in the hexane extract, followed in decreasing order by the chloroform, methanol, and dichloromethane extracts. It was concluded that the increase in concentration of the active substance increased the antioxidant activity of the extracts under study, with significant differences noted at 1 mg/mL compared to other concentrations in the different treatments (p < 0.05).
Conclusion: Low-polarity organic solvents are better solvents than high-polarity solvents for extracting antioxidants from L. obtusa, as they yield extracts with the highest in vitro activity. The extracts contain antioxidant compounds with a high electron-donating capacity and metal ion-chelating power. It is advisable to identify and purify the antioxidant compounds found in the chloroform and hexane extracts of L. obtusa.
Keywords: Laurencia obtusa, Marine algae, Antioxidant properties, Free radicals, Chelating, Reduction power
Type of Study: Research |
Subject:
Herbal Drugs
Received: 2026/02/13 | Accepted: 2026/03/25 | Published: 2026/01/1
Received: 2026/02/13 | Accepted: 2026/03/25 | Published: 2026/01/1
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