Volume 3, Issue 1 (6-2021)                   pbp 2021, 3(1): 1-8 | Back to browse issues page


XML Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Ghaneialvar H, Abbasi N, Saneei S, Zangeneh A, Zangeneh M M, Pooyanmehr M, et al . An Ethnomedicinal Plant: Antibacterial Activities of Stachys Lavandulifolia Vahl Aqueous Extract against Common Pathogens. pbp 2021; 3 (1) :1-8
URL: http://pbp.medilam.ac.ir/article-1-60-en.html
1- 1 Biotechnology and Medicinal Plants Research Center, Ilam University of Medical Sciences, Ilam, Iran2 Department of Clinical Biochemistry, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran , ghaneialvar-h@medilam.ac.ir
2- Biotechnology and Medicinal Plants Research Center, Ilam University of Medical Sciences, Ilam, Iran
3- Department of Dermatology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran
4- Department of Microbiology section, Pathobiology & Basic Sciences, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran.
5- Department of Clinical Sciences, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran
6- Department of Microbiology section, Pathobiology & Basic Sciences, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran
Abstract:   (2306 Views)
Enhancing microbial resistance to antibiotics and their probable side effects leads to the popularity of medicinal plants, so the need for novel antibacterial compounds with plant origin is felt more than ever. The object of the present study was to assess the antibacterial property of Stachys lavandulifolia Vahl aqueous extract (SLVAE) on Escherichia coli O157:H7 (EC), Pseudomonas aeruginosa (PA), Staphylococcus aureus (SA) and Bacillus subtilis (BS). The aqueous extract was obtained using a rotary evaporator. Agar disk and well diffusion methods were used to investigate the antibacterial property of the SLVAE. In the agar disk diffusion test, distilled water was used as a negative control whereas streptomycin, oxytetracycline, gentamicin, difloxacin, chloramphenicol, ampicillin, and amikacin were used as positive controls. Macro broth tube test was accomplished to specified Minimum Inhibitory Concentration (MIC). Statistical comparison among groups means were done through one-way ANOVA followed by Duncan’s post-hoc test. P 0.01 was considered as significant. Indeed compared with many standard antibiotics, the extract showed the higher antibacterial property. Also SLVAE with 125, 15/62 and 7/81 mg/mL concentrations has prevented the growth of EC, SA/BS and PA, respectively, and with 125, 62/5 and 15/62 mg/mL concentrations has destroyed EC, SA/BS and PA, respectively (p0.01). SLVAE had the most antibacterial activity on PA.  In conclusion the obtained results indicated the antibacterial effect of SLVAE on EC, PA, SA, and BS. It seems that this plant can be used for the treatment of some bacterial infections as an antibiotic.
 
Full-Text [PDF 2125 kb]   (407 Downloads)    
Type of Study: Research | Subject: Herbal Drugs
Received: 2020/06/18 | Accepted: 2020/12/28 | Published: 2021/07/6

References
1. Goorani S, Shariatifar N, Seydi N, Zangeneh A, Moradi R, Tari B, Nazari F, Zangeneh MM. The aqueous extract of Allium saralicum R.M. Fritsch effectively treat induced anemia: experimental study on Wistar rats. Orient Pharm Exp Med 2019; 19: 403-413.
2. Zangeneh A, Zangeneh MM, Goodarzi N, Najafi F, Hagh-Nazari L. Protective effects of aqueous extract of internal septum of walnut fruit on diabetic hepatopathy in streptozotocin-induced diabetic mice. Sci J Kurdistan Univ Med Sci. 2018; 23(1): 26-37.
3. Zangeneh MM, Zangeneh AA, Tahvilian R, Moradi R. Evaluation of the nephroprotective effect of Glycyrrhiza glabra L aqueous extract on CCl4-induced nephrotoxicity in mice. Comp Clin Pathol. 2018; 27:1119-1126.
4. Zangeneh MM, Zangeneh A, Salmani S, Jamshidpour R, Koosari F. Protection of phenylhydrazine-induced hematotoxicity by aqueous extract of Ocimum basilicum in Wistar male rats. Comp Clin Path. 2019; 28: 331-338.
5. Ghashghaii A, Hashemnia M, Nikousefat Z, Zangeneh MM, Zangeneh A. Wound healing potential of methanolic extract of Scrophularia striata in rats. Pharm Sci 2017; 23 (4): 256-63.
6. Foroughi A, Zangeneh MM, Kazemi N, Zangeneh A. An in vitro study on antimicrobial properties of Allium noeanumreut ex regel: An ethnomedicinal plant. Iranian J Publ Health 2016; 45(2): 32-32.
7. Hagh-Nazari L, Goodarzi N, Zangeneh MM, Zamgeneh A, Tahvilin R, Moradi R. Stereological study of kidney in streptozotocin-induced diabetic mice treated with ethanolic extract of Stevia rebaudiana (bitter fraction). Comp Clin Pathol 2017; 26 (2): 455-463.
8. Zangeneh MM, Zangeneh A, Amiri H, Amiri N, Tahvilian R, Moradi R, Zhaleh H, Razeghi Tehrani P. Nephroprotective activity of Alyssum meniocoides Boiss aqueous extract on streptozotocin-induced diabetic nephrotoxicity in male mice. Comp Clin Pathol. 2018; 27(5): 1147-1154.
9. Zangeneh MM, Zangeneh A, Tahvilian R, Moradi R, Hajialiani M, Moradi M, Simanizad S, Chamani P. Antibacterial activities of a medicinal plant. Int J Curr Med Pharm Res 2017; 3(3): 1471-4.
10. Mohammadi G, Zangeneh MM, Zangeneh A, Siavash-Haghighi ZM. Chemical characterization and anti‐breast cancer effects of silver nanoparticles using Phoenix dactylifera seed ethanolic extract on 7,12‐Dimethylbenz[a] anthracene‐induced mammary gland carcinogenesis in Sprague Dawley male rats. Appl. Organomet. Chem. 2020; 34(1): e5136.
11. Zangeneh MM, Zangeneh A, Tahvilian R, Moradi R, Kiani K, Khalili M, Shikhi T, Amiri A. Ethnomedicinal Plants: Evaluation of in vitro Antibacterial properties of Verbascum thapsus against Bacillus subtilis ATCC No. 21332. Int J Ayu Pharm Chem 2017; 6(3): 155-66.
12. Sherkatolabbasieh H, Hagh-Nazari L, Shafiezadeh S, Goodarzi N, Zangeneh MM, Zangeneh A. Ameliorative effects of the ethanolic extract of Allium saralicum R.M. Fritsch on CCl4-induced nephrotoxicity in mice: A stereological examination. Arch Biol Sci. 2017; 69(3):535-43.
13. Hemmati S, Rashtiani A, Zangeneh MM, Mohammadi P, Zangeneh A, Veisi H. Green synthesis and characterization of silver nanoparticles using Fritillaria flower extract and their antibacterial activity against some human pathogens. Polyhedron 2019; 158: 8–14.
14. Joshi A, Joshi VK, Pandey D, Hemalatha S. Systematic investigation of ethanolic extract from Leea macrophylla: implications in wound healing. J Ethnopharmacol. 2016; 191: 95–106, 2016. (b) Yariswamy M, Shivaprasad HV, Joshi V, Nanjaraj Urs AN, Nataraju A, Vishwanath BS. Topical application of serine proteases from Wrightia tinctoria R. Br. (Apocyanaceae) latex augments healing of experimentally induced excision wound in mice. J Ethnopharmacol. 2013: 149: 377–383 (c) de Castro Campos Pinto N, Cassini-Vieira P, de Souza-Fagundes EM, Barcelos LS, Castañon MSMN, Scio E, Pereskia aculeata Miller leaves accelerate excisional wound healing in mice. J Ethnopharmacol. 2016; 194: 131–136. (d) dos Santos Gramma LS, Marques FM, Vittorazzi C, Struthanthus vulgaris ointment prevents an over expression of inflammatory response and accelerates the cutaneous wound healing. J Ethnopharmacol, 2016; 190: 319–327. (e) Biswas TK, Pandit S, Chakrabarti S, Banerjee S, Poyra N, Seal T. Evaluation of Cynodon dactylon for wound healing activity. J Ethnopharmacol. 2017; 197: 128–137. (16f) Bhat PB, Hegde S, Upadhya V, Hegde GR, Habbu PV, Mulgund GS. Evaluation of wound healing property of Caesalpinia mimosoides Lam. J Ethnopharmacol. 2016; 193: 712–724.
15. Najafi F, Zangeneh MM, Tahvilian R, Zangeneh A, Amiri H, Amiri N, Moradi R. In vitro antibacterial efficacy of essential oil of Allium sativum against Staphylococcus aureus. Int J Pharm Phytochem Res 2016; 8(12): 2039-43.
16. Sayyedrostami T, Pournaghi P, Ebrahimi Vosta-Kalaee S, Zangeneh MM. Evaluation of the wound healing activity of Chenopodium botrys leaves essential oil in rats (A short-term study). J Essent Oil Bear Pl. 2018; 21(1): 164-74.
17. Zangeneh MM. Hematoprotective and nephroprotective properties of ethanolic extract of Anthemis odontostephana Boiss in streptozotocin-induced diabetic mice. J Med Plants 2018; 17(65): 47-60.
18. Seyyednejad SM and Motamedi H. A Review on Native Medicinal Plants in Khuzestan, Iran with Antibacterial Properties. Inter J Pharmaco 2010; 6: 551-60.
19. Skaltsa, H., Lazari, D. Chinou, I. and Loukis, A. Composition & antibacterial activity of the essential oils of Stachys eandica & S. chrysantha from southern Greece. Planta Medica 1999; 65(3): 255-6.
20. Hayashi K, Nagamatsu T, Ito M, Hattori T, Suzuki Y. Acteoside, a component of Stachys sieboldii MIQ, may be a promising antinephritic agent (1): Effects of acteoside on crescentic-type anti-GBM nephritis in rats. J Pharmacol Sci 1994; 65: (2) 143-51.
21. Miyase T, Yamamoto R, Ueno A. Phenylethanoid glycosides from Stachys officinalis. Phytochem 1996; 43(2):475-9.
22. Ross ST, Zinchenko FL. Study of triterpenoids and steroids of Stachys palustris. Farmatsevtychnyi Zhurnal (Kiev). 1975: 30 (1): 91-2.
23. Zinchenko TV. Flavonoid glycosides of Stachys neglecta. Farmatsevtychnyi Zhurnal (Kiev) 1970; 25(4):81-2.
24. Clinical and laboratory standards institute (CLSI). M7-A7 2006; 26 (2).
25. Zhaleh M, Sohrabi N, Zangeneh MM, Zangeneh A, Moradi R, Zhaleh H. Chemical composition and antibacterial effects of essential oil of Rhus coriaria fruits in the west of Iran (Kermanshah). J Essent Oil Bear Pl. Doi: 10.1080/0972060X.2018.1462739.
26. Javidnia K, Mojab F, Mojahedi SA. Chemical constituents of the essential oil of Stachys lavandulifolia Vahl from Iran. J Essent Oil Bear Pl 2003; 6(3): 174-178
27. Khanavi M, Sharifzade M, Hadjakhoondi A, Shafiee A. Phytochemical investigation and anti-inflammatory activity of aerial parts of Stachys byzantina C. koch. J Ethnopharmacol 2005; 97(3): 463-468.
28. Saeedi M, Morteza-Semnani K, Mahdavi MR, Rahimi F. Antimicrobial studies on extracts of four species of Stachys. Indian J Pharm Sci 2008; 70(3): 403-406.
29. Pirbalouti AG, Mohammadi M. Phytochemical composition of the essential oil of different populations of Stachys lavandulifolia Vahl. Asian Pac J Trop Biomed 2013; 3(2): 123-8.
30. Zarali M, Hojjati M, Didehban ST, Jooinadeh H. Evaluation of chemical composition and antibacterial activities of Echinophora cinerea Boiss and Stachys lavandulifolia Vahl essential oils in vitro. J Food Sci Tech 2015; 52(13): 1-12.
31. Taheri M, Mahdavi S, Mehmannavaz Y. Antibacterial activity of different extracts from the aerial parts and roots of Stachys lavandulifolia Vahl harvested at two stages of growth. J Herb Med 2014; 5(1): 7-12.
32. Mahzooni-kachapi S, Mahdavi M, Roozbeh-nasira L, Akbarzadeh M, Rezazadeh F, Motavalizadehkakhky A. Antimicrobial activity and chemical composition of essential oils of Stachys lavandulifolia Vahl from Mazandaran, Iran. J Med Plants Res 2012; 6(24): 4149-58.
33. Dugler G, Aki C. Antimicrobial activity of the leaves of endemic Stachys pseudopinardii in Turkey. Trop J Pharm Res 2009; 8(4): 371-375.

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.