fa Herbal Drugs پژوهشي Research <span style="font-size:10pt"><span style="line-height:150%"><span style="font-family:Optima"><span style="color:black"><b>Objective</b><b><span style="font-size:11.0pt"><span style="line-height:150%"><span calibri="" style="font-family:"><span style="color:black">:</span></span></span></span></b> <i><span style="color:black">Tapinanthus globiferus</span></i><span style="color:black"> is a hemi-parasitic plant used in ethonomedicine to treat different ailment including fungal infections</span><span style="color:black">. The aim of the study was to isolation the bioactive compound from leaf of <i>T.globiferus</i> growing on<i> Balanites</i> <i>aegyptiaca</i>. &nbsp;&nbsp;&nbsp;</span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:150%"><span style="font-family:Optima"><span style="color:black"><b>Material and Methods</b><span style="color:black">: The</span><span style="font-size:11.0pt"><span style="line-height:150%"><span calibri="" style="font-family:"><span style="color:black"> plant</span></span></span></span> <span style="color:black">material was collected, identified pulverized to powder using mortar and pestle. The powdered plant material was subjected to maceration with 90% methanol to obtained crude methanol leaf extract which was further partitioned with solvent of increasing polarity to obtained <i>n</i>-hexane, chloroform, ethylacetate and <i>n</i>-butanol fractions.</span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:150%"><span style="font-family:Optima"><span style="color:black"><b>Results</b><b><span calibri="" style="font-family:"><span style="color:black">: </span></span></b><span style="color:black">Chromatographic separation of ethylacetate fraction using a combination of silica gel column and sephadex LH-20 column led to the isolation of flavonoid (3,5,7- trihydroxy-2-(4-hydroxyphenyl)-4H-chromen-4-one). The structure of the compound obtained was established by Spectroscopic analysis (FTIR, NMR). </span></span></span></span></span><br> <b><span style="font-size:11.0pt"><span style="line-height:107%"><span calibri="" style="font-family:">Conclusion</span></span></span><span style="font-size:11.0pt"><span style="line-height:107%"><span calibri="" style="font-family:">:</span></span></span></b> <span style="font-size:11.0pt"><span style="line-height:107%"><span style="font-family:Optima"><span style="color:black">Chromatographic studies of ethyl acetate fraction of <i>T. globiferus</i> growing on <i>Balanites aegyptiaca</i>, afforded a 3,5,7-trihydroxy-2-(4-hydroxyphenyl)-4H-chromen-4-one.To the best of our knowledge, this is the first report of isolation of this compound from <i>Tapinanthus</i> <i>globiferus</i> growing on <i>Balanites</i> <i>aegyptiaca</i>.</span></span></span></span> Isolation, Balanite aegyptiaca, Tapinanthus globiferus, NMR, FTIR 43 47 http://pbp.medilam.ac.ir/browse.php?a_code=A-10-455-2&slc_lang=fa&sid=1 Mukhtar T tukur.mukhtar@ssu.edu.ng 10031947532846002065 10031947532846002065 No Department of Chemistry, Sokoto State University, Sokoto Nigeria Mansur Y.I. Y.I.mansur@ymail.com 10031947532846002066 10031947532846002066 Yes Department of Chemistry, Sokoto State University, Sokoto Nigeria Abubakar H abubakarh@ymail.com 10031947532846002067 10031947532846002067 No Department of Chemistry, Sokoto State University, Sokoto Nigeria Yusuf A. J ajyusuf@ymail.com 10031947532846002068 10031947532846002068 No Department Pharmaceutical and Medicinal Chemistry, Usmanu Danfodoyo University, Sokoto, Nigeria