fa Herbal Drugs پژوهشي Research <span style="font-size:10pt"><span style="line-height:150%"><span optima="" style="font-family:"><span style="color:black"><b>Objective:</b> Thus, in this work, phytochemical screening, acute toxicity and antifungal evaluation on the methanol extracts of different parts (leaves, seed and aerial part) of <i>Mitracarpus</i> <i>hirtus</i> against <i>Candida</i> <i>albicans</i>, <i>Candida</i> <i>tropicalis</i>, <i>Aspergillus</i> <i>fumigatus</i>, <i>Aspergillus</i> <i>niger</i>, <i>Histoplasm</i> <i>capsulatum</i>, <i>Mucor</i> sp, <i>Microsporum</i> <i>canis</i>, <i>Microsporum</i> <i>gallinae</i>, <i>Trichophyton</i> <i>mentagrophyte</i>, and <i>Trichophyton</i> <i>rubrum</i> using agar well diffusion and broth dilution techniques.</span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:150%"><span optima="" style="font-family:"><span style="color:black"><b>Material and Methods:</b> The phytochemical screening of the methanol extracts of different parts of the plant revealed the presence of tannins saponins, flavonoids, alkaloids, steroids and terpenoids and the median lethal dose (LD50) was estimated to be greater than 5000 mg/kg which indicates that the plant is non-toxic.</span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:150%"><span optima="" style="font-family:"><span style="color:black"><b>Results:</b> Susceptibility test for the methanol extracts showed mean zone of inhibition ranging from 19.33&plusmn;0.58-23.33&plusmn;0.56 mm against the test organisms while the standard drug, Fulcin had 24.33&plusmn;0.58 &ndash; 31.67&plusmn;0.58 mm. The minimum inhibitory concentration (MIC) of the extracts ranges between 0.25-0.50 mg/mL while the minimum fungicidal concentration (MFC) ranges between 0.25-1.00 mg/mL against some of the fungal pathogens.</span></span></span></span><br> <b><span style="font-size:11.0pt"><span style="line-height:107%"><span calibri="" style="font-family:">Conclusion:</span></span></span></b><span style="font-size:11.0pt"><span style="line-height:107%"><span calibri="" style="font-family:"> The findings of this study have revealed the antifungal efficacy of methanol extracts of the different part of <i>M. hirtus</i> which supports the ethno-medicinal claim of the use of the plant in the treatment of fungal infections.</span></span></span> Mitracarpus hirtus, Secondary metabolites, Antifungal, Toxicity 48 56 http://pbp.medilam.ac.ir/browse.php?a_code=A-10-452-8&slc_lang=fa&sid=1 Abubakar Hassan hassan.abubakar@ssu.edu.ng 10031947532846002069 10031947532846002069 Yes Department of Chemistry, Sokoto State University, Sokoto, Nigeria Alam O. oalam@jamiahamdard.ac.in 10031947532846002070 10031947532846002070 No 2Department of Pharmaceutical Chemistry, Jamia Hamdard, New Delhi, India Tukur M tukur.mukhtar@ssu.edu.ng 10031947532846002071 10031947532846002071 No Department of Chemistry, Sokoto State University, Sokoto, Nigeria Muazu A. S safgummi@yahoo.com 10031947532846002072 10031947532846002072 No Department of Chemistry, Sokoto State University, Sokoto, Nigeria Yusuf A. J. amina.yusuf@udusok.edu.ng 10031947532846002073 10031947532846002073 No Department of Pharmaceutical and Medicinal Chemistry, Usmanu Danfodiyo University, Sokoto, Nigeria