fa Herbal Drugs پژوهشي Research <span style="font-size:10pt"><span style="line-height:150%"><span style="font-family:Optima"><span style="color:black"><b>Objective:</b> The objective of this research was focused on the development of new organoclay based composite that serves as both antibacterial and dye removing agent for the treatment of microbial and dyes contaminated water from the source.</span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:150%"><span style="font-family:Optima"><span style="color:black"><b>Material and Methods:</b> The cation exchange capacity (CEC) of the kaolinite was improved through acid treatment. Chlorhexidine- loaded zinc- kaolinite was prepared via adsorption of chlorhexidine acetate (0.5 mmol/L) on zinc-kaolinite. The composites were characterized using Field Emission Scanning Electron Microscopy (FESEM) and Energy Dispersive X-ray (EDX). The antibacterial assays of the composites were conducted against Staphylococcus aureus (S. aureus) and Salmonella typhi (S. typhi) using disc diffusion technique (DDT), minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC).</span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:150%"><span style="font-family:Optima"><span style="color:black"><b>Results: </b>The CEC value of the acid treated kaolinite (Kaot₂) was improved from 9.26 <u>+</u> 0.82 to 13.43<u>+</u>1.61 meq/100g, the morphology of the composite remains intact and indicate the presence of Zinc (Zn) after formulation. The target composite (Chx-Zn-Kaot₂) shows its effectiveness against <i>S. aureus</i> and <i>S. typhi</i> showing the inhibition zones of 26 mm and 1.5 mm respectively. Similarly, MIC, with 120 mg/mL inhibit both organisms while MBC revealed that the target composite, 60 mg/mL kills <i>S. aureus</i> and 120 mg/mL kills <i>S. typhi</i> respectively. </span></span></span></span><br> <b><span style="font-size:11.0pt"><span style="line-height:107%"><span calibri="" style="font-family:">Conclusion:</span></span></span></b><span style="font-size:11.0pt"><span style="line-height:107%"><span calibri="" style="font-family:"> The formulated target composite is a good candidate for the treatment of drinking water contaminated with such microorganisms and can be able to remove substantial content of dyes.</span></span></span> MTHFR C677T, MTHFR A1298C, FV G1691A, FII G20210A, PAI-1 -675 4G/5G, Recurrent pregnancy loss 103 113 http://pbp.medilam.ac.ir/browse.php?a_code=A-10-25-35&slc_lang=fa&sid=1 Hourieh Khani hourikhani@gmail.com 10031947532846002092 10031947532846002092 No Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran Mohammad Ali Hosseinpour Feizi pourfeizi@eastp.ir 10031947532846002093 10031947532846002093 Yes Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran Jafar Mohseni jmohseni46@gmail.com 10031947532846002094 10031947532846002094 No Assisted Reproductive Technology (ART) Center, Eastern Azerbaijan Branch of Academic Center for Education, Culture and Research (ACECR), Tabriz, Iran Mehdi Haghi mehdihaghi@tabrizu.ac.ir 10031947532846002095 10031947532846002095 No Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran Reza Safaralizadeh safaralizadeh@tabrizu.ac.ir 10031947532846002096 10031947532846002096 No Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran