fa <span style="font-size:10pt"><span style="line-height:150%"><span optima="" style="font-family:"><span style="color:black"><b>Objective:</b> Liver, as an organ, is predominantly responsible for the metabolism of drugs, alcohol, and foreign chemicals; hence, it is vulnerable to injury that may result in different liver diseases. The protective effect of <i>Chromolaena</i> <i>odorata</i> (<a href="https://en.wikipedia.org/wiki/Carl_Linnaeus" title="Carl Linnaeus"><span style="color:black"><span style="text-decoration:none"><span style="text-underline:none">L.</span></span></span></a>) <a href="https://en.wikipedia.org/w/index.php?title=Robert_Merrill_King&action=edit&redlink=1" title="Robert Merrill King (page does not exist)"><span style="color:black"><span style="text-decoration:none"><span style="text-underline:none">R.M. King</span></span></span></a> & <a href="https://en.wikipedia.org/wiki/Harold_E._Robinson" title="Harold E. Robinson"><span style="color:black"><span style="text-decoration:none"><span style="text-underline:none">H. Rob.</span></span></span></a> aqueous leaf extract against Methotrexate-induced hepatotoxicity in male wistar rats was investigated.</span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:150%"><span optima="" style="font-family:"><span style="color:black"><b>Material and Methods:</b> 24 male rats were divided into four groups of 6. Group one received normal saline only and served as control while group two received 200mg/kg <i>Ch. odorata</i> aqueous extract daily for ten consecutive days. Group three received intraperitoneally, a single dose of 20mg/kg Methotrexate on the ninth day of treatment while group four received 200mg/kg <i>Ch. Odorata</i> aqueous extract for ten consecutive days and a single dose of 20mg/kg Methotrexate intraperitoneally on ninth day of the study.</span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:150%"><span optima="" style="font-family:"><span style="color:black"><b>Results:</b> Results showed that Methotrexate-induced hepatotoxicity decreased liver synthetic molecules (Total Protein and Albumin); decreased endogenous antioxidants (Catalase and Superoxide dismutase); increased liver function enzymes (Alanine aminotransferase and Aspartate aminotransferase), and increased lipid peroxidation (Malondialdehyde). Corroborating biochemical assessment, Histopathological analysis of untreated Methotrexate rats showed liver sections with focal necrosis, dilation and congestion of central vein and portal vein whereas aqueous leaf extract of <i>Ch. odorata</i> reduced the degree of lesions with seen improvement and reduction of hepatocytes degeneration.</span></span></span></span><br> <b><span style="font-size:11.0pt"><span style="line-height:107%"><span calibri="" style="font-family:">Conclusion:</span></span></span></b><span style="font-size:11.0pt"><span style="line-height:107%"><span calibri="" style="font-family:"> Thus, <i>Ch. odorata</i> (L.) offered protection to the liver from damage caused by Methotrexate attributable to its active bioactive agents including flavonoids which scavenges free radicals, enhanced the antioxidant status and protected against oxidative damage and oxidative stress.</span></span></span> Antioxidant,Chromolaena odorata,Liver,Methotrexate,Oxidative stress 12 21 http://pbp.medilam.ac.ir/browse.php?a_code=A-10-446-2&slc_lang=fa&sid=1 Usunomena Usunobun usunobun.usunomena@edouniversity.edu.ng 10031947532846002048 0000000297939115 Yes Edo State University Uzairue, Nigeria Ochuwa Imoru darkcupid2000@gmail.com 10031947532846002049 0000000297939115 No Edo State University Uzairue, Edo State, Nigeria