en Pharmacognosy پژوهشي Research <span style="font-size:10pt"><span style="line-height:150%"><span optima="" style="font-family:"><span style="color:black"><b><span style="font-size:9.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">Objectives:</span></span></span></b><span style="font-size:9.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""> The role of <i>Ocimum canum</i> Sim., as a medicinal agent used for the treatment and management of diseases cannot be overemphasized. The aim of the study is to screen the Phytochemical constituents and to evaluate the antiepileptic effect of methanol whole plant extract in Wistar albino rats.</span></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:150%"><span optima="" style="font-family:"><span style="color:black"><b><span style="font-size:9.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">Material and Methods:</span></span></span></b><span style="font-size:9.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""> The whole plant part of <i>O. canum</i> was extracted with 85% percentage of methanol and screened for phytochemicals. An acute toxicity study was carried out using Lorke&rsquo;s method and the antiepileptic activity was evaluated using pentylenetetrazole (PTZ) and strychnine in rats. One-way analysis of variance (ANOVA) was used in the analysis of the data.</span></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:150%"><span optima="" style="font-family:"><span style="color:black"><b><span style="font-size:9.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">Results:</span></span></span></b><span style="font-size:9.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""> The phytochemical analysis revealed the presence of saponins, cardiac glycosides, flavonoids, terpenoids, and cardenolides. The intraperitoneal median lethal dose value (LD50) of <i>O. canum</i> in rats was 2154 mg/kg. The extract at the dose of 300 and 600 mg/kg body weight protected 40% and 80% of experimental animals against both PTZ and strychnine-induced convulsion; protected 60% and 80% of rats against death induced by strychnine; compared to 100% protection by the standard drug sodium valproate (20mg/kg).</span></span></span></span></span></span></span><br> <b><span style="font-size:9.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">Conclusion:</span></span></span></b><span style="font-size:9.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""> The antiepileptic investigation suggests that methanol extract of whole plant of <i>O. canum</i> has antiepileptic activity which could be employed for the management of epileptic related conditions</span></span></span> Ocimum canum,Phytochemical,Antiepileptic,Pentylenetetrazole (PTZ),Strychnine 93 102 http://pbp.medilam.ac.ir/browse.php?a_code=A-10-574-1&slc_lang=en&sid=1 James Yakubu jamesyakubu96@gmail.com 10031947532846004981 000000017507546X Yes Department of Pure and Applied Chemistry, Faculty of Science, University of Maiduguri, PMB, 1069, 1069, Maiduguri, Borno State, Nigeria. Abdullahi Mbaya Hassan abdullahihassan770@gmail.com 10031947532846004982 0009000661423332 No Department of Pure and Applied Chemistry, Faculty of Science, University of Maiduguri, PMB, 1069, 1069, Maiduguri, Borno State, Nigeria. Hassan Braimah Yesufu hassanyesufubraimah@gmail.com 10031947532846004983 0000000343517707 No Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Maiduguri, PMB, 1069, Maiduguri, Borno State, Nigeria. Fanna Inna Abdulrahman fannas06@gmail.com 10031947532846004984 0000000161838358 No Department of Pure and Applied Chemistry, Faculty of Science, University of Maiduguri, PMB, 1069, Maiduguri, Borno State, Nigeria Olufunke Adebola Sodipo sodipoolufunke@yahoo.com 10031947532846004985 0000000332308969 No Department of Clinical Pharmacology and Therapeutics, Faculty of Basic Clinical Sciences, College of Medical Sciences, University of Maiduguri, Borno State, Nigeria. Asinamai Medugu Ndai a.ndai@ufl.edu 10031947532846004986 0000000225173915 No Department of Pharmaceutical Outcomes and Policy, College of Pharmacy, University of Florida, Florida, USA. Bitrus Wampana bwampana@gmail.com 10031947532846004987 0000000228586096 No Department of Pharmacology, Faculty of Veterinary Medicine, University of Maiduguri, Nigeria