fa <span style="font-size:14px;"><span style="font-family:Times New Roman;"><span style="line-height:107%"><span calibri=""><b><span lang="EN" new="" roman="" times="">Objective:</span></b><span lang="EN" new="" roman="" times=""> Attention to the daily prescription of large amounts of drugs by doctors; In recent years, much attention has been paid to the effect of plants in the treatment and prevention of drug poisoning. <i>Matricaria chamomilla</i> is one of the oldest medicinal plants known to man and its use dates back to ancient Greece</span><span dir="RTL" lang="AR-SA" new="" roman="" times="">.</span> <span lang="EN" new="" roman="" times="">In this research, the protective effect of green tea extract in hepatotoxicity caused by acetaminophen has been investigated. </span></span></span><br> <span style="line-height:107%"><span calibri=""><b><span lang="EN" new="" roman="" times="">Methods:</span></b><span lang="EN" new="" roman="" times=""> So that 48 adult male mice were randomly divided into 6 groups. The control group received only physiological serum.</span> <span lang="EN" new="" roman="" times="">The <i>M. chamomile</i> group received 250 mg/kg and (AgNP)M chamomilla received 0.5 mg/kg for 30 days. The acetaminophen group was prescribed 500 mg/kg of acetaminophen orally, and the experimental groups (acetaminophen + <i>M. chamomile </i>extract) were given chamomile extract with a dose of 250 mg/kg and (silver nanoparticle of <i>M. chamomile</i> plant + acetaminophen) (AgNP) <i>M. chamomilla</i> with a dose of 0.5 received mg/kg for 30 days and a toxic dose of acetaminophen was prescribed on the 29th day. </span></span></span><br> <span style="line-height:107%"><span calibri=""><b><span lang="EN" new="" roman="" times="">Results:</span></b><span lang="EN" new="" roman="" times=""> On the 31st day, blood was drawn to measure alanine transferase (ALT) and aspartate transferase (AST), and after that, the animals&#39; livers were placed in 10% formalin for histopathological examination. The serum levels of ALT and AST enzymes in the silver nanoparticle group were significantly reduced compared to other groups. (P<0.05) so that in histopathological studies, liver necrosis, congestion of red blood cells and the accumulation of inflammatory cells in (AgNP) <i>M. chamomilla</i> were reduced compared to the acetaminophen group. </span></span></span><br> <span style="line-height:107%"><span calibri=""><b><span lang="EN" new="" roman="" times="">Conclusion:</span></b><span lang="EN" new="" roman="" times=""> According to the results of this research (AgNP)M chamomilla&nbsp;&nbsp; is probably involved in liver necrosis caused by acetaminophen. It has a protective role.</span></span></span></span></span> Hepatotoxicity, Acetaminophen, (AgNP) M. chamomilla 122 130 http://pbp.medilam.ac.ir/browse.php?a_code=A-10-25-44&slc_lang=fa&sid=1 Mohammad Mehdi Zangeneh m.mahdizangeneh@gmail.com 10031947532846005023 10031947532846005023 No Biotechnology and Medicinal plants Research Center Ilam University of Medical Sciences,Ilam, Iran Navid Etemadi netemadi@ymail.com 10031947532846005024 10031947532846005024 No DVM Graduate, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran Samira Zand szand@ymail.com 10031947532846005025 10031947532846005025 No Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran Ali Pirnejad-Talatapeh atalatapeh@ymail.com 10031947532846005026 10031947532846005026 No Central Laboratory, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran Akram Zangeneh azangeneh@ymail.com 10031947532846005027 10031947532846005027 Yes Biotechnology and Medicinal plants Research Center Ilam University of Medical Sciences,Ilam, Iran Ghobad Abangah ilamfarma@gmail.com 10031947532846005028 10031947532846005028 No School of Medicine, Shahid Mostafa Khomaeini Hospital, Ilam University of Medical Sciences, Ilam, Iran Hori Ghaneialvar 10031947532846005029 10031947532846005029 No Biotechnology and Medicinal plants Research Center Ilam University of Medical Sciences,Ilam, Iran