en Bioactive Compounds پژوهشي Research <p data-end="595" data-start="124" style="text-align: justify;"><span style="font-size:14px;"><span style="font-family:Times New Roman;"><strong data-end="138" data-start="124">Objective:</strong>&nbsp;Intravenous albumin administration is known to improve blood circulation and promote wound healing, while topical albumin can inhibit bacterial growth on wounds. Additionally, topical caffeine has been reported to stimulate vascularization and enhance blood vessel formation in the skin. This study aimed to investigate, for the first time, the effect of topical albumin and caffeine individually and in combination&mdash;on wound healing rate in rat models.</span></span></p> <p data-end="999" data-start="597" style="text-align: justify;"><span style="font-size:14px;"><span style="font-family:Times New Roman;"><strong data-end="609" data-start="597">Methods:</strong>&nbsp;Albumin- and caffeine-loaded nanofibers were fabricated using the electrospinning technique. Nanofiber morphology was characterized using scanning electron microscopy (SEM) and their chemical composition was confirmed by Fourier-transform infrared spectroscopy (FT-IR). The therapeutic effects of the prepared nanofibers were evaluated in a full-thickness excision wound model in rats.</span></span></p> <p data-end="1321" data-start="1001" style="text-align: justify;"><span style="font-size:14px;"><span style="font-family:Times New Roman;"><strong data-end="1013" data-start="1001">Results:</strong>&nbsp;Both albumin alone and the albumin&ndash;caffeine combination significantly accelerated wound healing, with a marked reduction in the wound area compared to controls. By the end of week 2, wound contraction reached 96% in the albumin&ndash;caffeine nanofiber group, compared with 79% in the untreated control group.</span></span></p> <p data-end="1640" data-start="1323" style="text-align: justify;"><span style="font-size:14px;"><span style="font-family:Times New Roman;"><strong data-end="1338" data-start="1323">Conclusion:</strong>&nbsp;Topical application of albumin-loaded nanofiber dressings demonstrated potent wound-healing properties, further enhanced by the addition of caffeine. These findings suggest that albumin&ndash;caffeine nanofiber dressings could represent a promising and innovative approach for accelerating wound repair.</span></span></p> <div style="text-align: justify;"></div> ,Albumin,caffeine,nanofiber,wound healing 1 7 http://pbp.medilam.ac.ir/browse.php?a_code=A-10-917-1&slc_lang=en&sid=1 Fatemeh Saadat fatemeh.saadat@yahoo.com 10031947532846005538 0009-0000-4228-4812 No Student Research Committee, Urmia University of Medical Sciences Anahita Fathi Azarbayjani anahitaazarbayjani@yahoo.com 10031947532846005539 10031947532846005539 Yes Department of Pharmaceutics, School of Pharmacy, Urmia University of Medical Sciences