en Green Chemistry پژوهشي Research <div style="text-align: justify;"><span style="font-size:14px;"><span style="font-family:Times New Roman;"><b>Objective:</b> The emergence of resistance in bacteria, and the existence of various types of infection and contamination in the hospital and community is the basis for research to find new antibacterial compounds.<span lang="FA" dir="RTL" style="font-family:&quot;Times New Roman&quot;,serif"></span><br> &nbsp;<b>Materials:</b> Ethyl Octahydrospiro[indene-2,3&#39;-pyrrolizidine]-1,3-diones-pyrrolizidine]-2&#39;carboxylate (5) was synthesized during one-pot reaction of ninhydrin, proline and ethyl acrylate in ethanol solvent. The antibacterial effect of the synthesized derivative on the mentioned bacteria was investigated by agar plate culture and broth micro dilution methods and the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined for the synthesized derivative. <span lang="FA" dir="RTL" style="font-family:&quot;Times New Roman&quot;,serif"></span><br> <b>Results:</b> The analysis of the results showed that the MIC and MBC values of the newly synthesized compound against Staphylococcus aureus (S. aureus) bacteria were 1.56 and 3.12 &mu;g/mlL, respectively. In addition, the MIC and MBC values of the synthetic compound against Escherichia coli (E. coli) were 0.39 &mu;g/mlL and 0.78 &mu;g/mlL, respectively. <span lang="FA" dir="RTL" style="font-family:&quot;Times New Roman&quot;,serif"></span><br> <b>Conclusion:</b> The antimicrobial test results revealed that the synthesized compound at an equivalent concentration exhibited a higher inhibitory impact on the Gram-negative E. coli bacteria than Gram-positive S. aureus. The compound causes the rapid bactericidal effect on Gram-negative bacteria by destroying or disrupting the function of the outer membrane. This combination could be effective in treating infections caused by Gram-negative bacteria.</span></span><br> &nbsp;</div> chiral azomethylene ylide,3 dipolar cyclization,Escherichia coli,indane 101 108 http://pbp.medilam.ac.ir/browse.php?a_code=A-10-661-3&slc_lang=en&sid=1 Armin Karimi armin.karimi.farsad@me.com 10031947532846005704 10031947532846005704 No Student Faculty of Pharmacy, Urmia University of Medical Sciences Reza Akbari akbarireza43@yahoo.com 10031947532846005705 10031947532846005705 No Department of Microbiology and Virology, School of Medicine, Urmia University of MedicalSciences, Urmia, Iran mehri kouhkan mehrikouhkan@gmail.com 10031947532846005706 10031947532846005706 Yes Department of Medicinal Chemistry, School of Pharmacy, Urmia University of Medical Sciences,Urmia, Iran.