fa Clinical پژوهشي Research <div style="text-align: justify;"><span style="font-size:14px;"><span style="font-family:Times New Roman;"><b>Objective:</b> Wound healing is a vital physiological process that maintains the skin&rsquo;s barrier function and prevents infection. The emergence of nanotechnology, particularly metallic nanoparticles, has introduced new strategies to enhance and accelerate wound repair. Among these, zinc oxide nanoparticles coated with acetylsalicylate have attracted attention due to their combined antibacterial and regenerative properties, positioning them as a promising therapeutic option in wound management. This study aimed to evaluate the effects of acetylsalicylate-coated zinc oxide nanoparticles on wound healing in male rats, with an emphasis on histopathological outcomes.<br> <b>Methods:</b> Wound repair involves a complex interplay of cellular and biochemical events leading to the restoration of tissue integrity. Forty-eight healthy male Wistar rats of uniform weight were randomly assigned to four groups (n=12 each) after standardized wound induction: Group I (control, no treatment), Group II (treated with standard phenytoin ointment), Group III (treated with base ointment), and Group IV (treated with acetylsalicylate-coated zinc oxide nanoparticles). Treatments were applied once daily for seven consecutive days. Histopathological assessments were conducted on days 7, 14, and 21 post-wounding.<br> <b>Results:</b> Group IV demonstrated significantly improved histopathological indices and accelerated wound healing compared with the other groups (P < 0.05). The findings indicate that acetylsalicylate-coated zinc oxide nanoparticles possess potent anti-inflammatory, antibacterial, and tissue-regenerative effects that substantially enhance wound repair in this animal model. Notably, epidermal and dermal regeneration across different healing phases was markedly improved, underscoring the efficacy of this nanocomposite.<br> <b>Conclusion:</b> Acetylsalicylate-coated zinc oxide nanoparticles may serve as a novel and effective therapeutic modality for wound management, particularly in infected wounds. Nevertheless, additional preclinical studies and clinical trials are required to confirm these effects and support their translation to human clinical applications.<br> <br> <img alt="" src="./files/site1/images/copuediting.png" style="width: 586px; height: 265px;" ><br> &nbsp;</span></span></div> Wound healing, Wound, Zinc oxide nanoparticles, Acetylsalicylate, Rat 0 0 http://pbp.medilam.ac.ir/browse.php?a_code=A-10-423-14&slc_lang=fa&sid=1 Kazem Rakhshan 10031947532846005917 10031947532846005917 No Student Research Committee, Lorestan University of Medical Sciences, Khorramabad, Iran Abdolrazagh Marzban 10031947532846005918 10031947532846005918 No Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran Zahra Haghighatian 10031947532846005919 10031947532846005919 No Department of Pathology, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran Abbas Raisi 10031947532846005920 10031947532846005920 No Department of Clinical Sciences, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran Leila Zarei leilazarei652@yahoo.com 10031947532846005921 10031947532846005921 Yes Palliative Care Research Center, Ur.C., Islamic Azad University, Urmia, Iran Asadollah Roshani mohamad@gmail.com 10031947532846005922 10031947532846005922 No Department of Surgery, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran