en Herbal Drugs پژوهشي Research <div style="text-align: justify;"><span style="font-size:14px;"><span style="font-family:Times New Roman;"><span style="line-height:115%"><b>Objective:</b> <i>Mucuna pruriens</i>, commonly known as velvet bean, is a climbing legume of the Fabaceae family. This study aimed to perform qualitative and quantitative phytochemical analyses and to identify the bioactive compounds present in the ethanolic seed extract of <i>M. pruriens</i> using GC-MS.</span><br> <span style="line-height:115%"><b>Methods:</b> Dried <i>M. pruriens</i> seed powder was extracted with ethanol at a 1:10 ratio using cold maceration. The extract was concentrated to dryness in a hot water bath over 72 hours. GC-MS analysis was conducted using Agilent Technologies equipment to characterize the phytochemical constituents. Identified compounds were compared against the National Institute of Standards and Technology (NIST) library for verification.</span><br> <span style="line-height:115%"><b>Results:</b> Preliminary phytochemical screening indicated the presence of alkaloids, flavonoids, phenols, saponins, steroids, tannins, and terpenoids. GC-MS analysis revealed five major bioactive compounds in the ethanolic seed extract.</span><br> <span style="line-height:115%"><b>Conclusion:</b> The ethanolic extract of <i>M. pruriens</i> seeds contains diverse bioactive phytochemicals, highlighting its potential as a source of natural compounds with therapeutic and pharmacological relevance. Further studies are warranted to explore the biological activities of the identified constituents</span><br> <img alt="" src="./files/site1/images/copuediting.png" style="width: 586px; height: 265px;" ></span></span></div> Mucuna pruriens,phytochemical analysis,GC-MS analysis,bioactive compounds,antioxidant activity 0 0 http://pbp.medilam.ac.ir/browse.php?a_code=A-10-1522-1&slc_lang=en&sid=1 Yordanos Germame yordanosgermame@gmail.com 10031947532846006084 10031947532846006084 Yes Researcher at Ethiopian Biodiversity Institute, Ethiopia Hailu Atnafu hailuatnafu@gmail.com 10031947532846006085 10031947532846006085 No Researcher at Ethiopian Biodiversity Institute, Ethiopia