en Green Chemistry پژوهشي Research <span style="font-family:Times New Roman;"><span style="font-size:14px;"><span style="line-height:normal"><b><span new="" roman="" times="">Background:</span></b><span new="" roman="" times=""> This study reports on the green synthesis of silver nanoparticles (AgNPs) using <i>Moringa oleifera</i> leaf extract as a natural reducing and stabilizing agent. The synthesized AgNPs were comprehensively characterized by UV&ndash;visible spectroscopy, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), field emission scanning electron microscopy (FESEM), energy dispersive X-ray spectroscopy (EDX), transmission electron microscopy (TEM), and zeta potential (ZP) analysis. </span></span><br> <span style="line-height:normal"><b><span new="" roman="" times="">Method:</span></b><span new="" roman="" times=""> AgNPs was synthesized through green approach using <i>M. oleifera</i> leaves extract as the source of reducing and stabilizing agent followed by its combination with some selected antibiotics and assess their inhibition modes.</span></span><br> <span style="line-height:normal"><b><span new="" roman="" times="">Objective: </span></b><span new="" roman="" times="">To determine the antibacterial mode of inhibition of AgNPs upon combination with antibiotics.</span></span><br> <span style="line-height:normal"><b><span new="" roman="" times="">Result:</span></b><span new="" roman="" times=""> The characterization confirmed the formation of uniformly distributed, spherical AgNPs with an average diameter of ~20 nm and a negative surface charge of &ndash;27.1 mV, indicating a high colloidal stability. To investigate their biomedical relevance, AgNPs were combined with conventional antibiotics via an imbibition method and tested against <i>Staphylococcus aureus</i> <i>(S. aureus</i>), methicillin-resistant <i>S. aureus</i> (MRSA), <i>Escherichia coli</i> (<i>E. coli</i>), and <i>Pseudomonas aeruginosa</i> (<i>P. aeruginosa</i>) using the Disc diffusion test (DDT).</span></span><br> <span style="line-height:normal"><span new="" roman="" times="">&nbsp;<b>Conclusion:</b> The results showed different antibacterial responses, with certain AgNPs&ndash;antibiotic combinations exhibiting synergistic enhancement of inhibition zones, while others showed antagonistic interactions. These results highlight the potential of <i>M. oleifera</i>-mediated AgNPs as an environmentally friendly nanomaterial to enhance antibiotic activity but also emphasize the need for critical evaluation of nanoparticle&ndash;drug interactions prior to clinical application.<br> <img alt="" src="./files/site1/images/copuediting.png" style="width: 586px; height: 265px;" ></span></span><br> &nbsp;</span></span> ,Moringa oleifera,silver nanoparticles,antibiotic,antagonistic,antibacterial effect 0 0 http://pbp.medilam.ac.ir/browse.php?a_code=A-10-1497-6&slc_lang=en&sid=1 Mustapha Isah imustapha@graduate.utm.my 10031947532846005953 10031947532846005953 Yes Department of Biosciences, Faculty of Science, Universiti Teknologi Malaysia, 81310 UTM Skudai, Johor, Malaysia Shafiu Abdullahi Namadi 10031947532846005954 10031947532846005954 No Department of Biochemistry and Molecular Biology, Sokoto State University, Birnin Kebbi Rd, Sokoto PMB 2134, Sokoto, Nigeria Fatimah Ibrahim Jumare 10031947532846005955 10031947532846005955 No Department of Biosciences, Faculty of Science, Universiti Teknologi Malaysia, 81310 UTM Skudai, Johor, Malaysia