en Ethno-medicine پژوهشي Research <span style="font-size:14px;"><span style="font-family:Times New Roman;"><span style="line-height:200%"><span sans-serif=""><b><span style="line-height:200%"><span new="" roman="" times="">Objectives:</span></span></b><i><span style="line-height:200%"><span new="" roman="" times=""> Cussonia spicata</span></span></i><span style="line-height:200%"><span new="" roman="" times=""> <span style="background:white"><span style="color:black">(</span></span>Araliaceae<span style="background:white"><span style="color:black"> family) has traditionally been used </span></span>in the ethno-medicine, especially in the ethno-veterinary medicine. We expected that <i>C. spicata </i>may have antioxidant activity due to its applications in the traditional medicine<span style="background:white"><span style="color:black">. </span></span>Therefore, in the present study, we aimed to evaluate the antioxidant potential, to determine the <span style="background:white"><span style="color:black">IC₅₀ values, </span></span>to estimate <span style="background:white"><span style="color:black">total phenolic contents (TPCs) and total flavonoid contents (TFCs) </span></span>of various solvent extracts obtained separately from the leaves and stem-bark of <i>C. spicata</i> collected in the Kingdom of Eswatini.</span></span></span></span><br> <span style="line-height:200%"><span sans-serif=""><b><span style="background:white"><span style="line-height:200%"><span new="" roman="" times="">Methods:</span></span></span></b> <span style="line-height:200%"><span new="" roman="" times="">Maceration technique together with hot solvent extraction technique was used to obtain various solvent extracts. DPPH radical scavenging assay was used to assess the antioxidant potential and determine the IC₅₀ values. </span></span><span lang="EN-ZA"><span style="line-height:200%"><span new="" roman="" times="">Folin-Ciocalteu colorimetric and aluminium chloride colorimetric methods were employed to </span></span></span><span style="background:white"><span style="line-height:200%"><span new="" roman="" times="">quantify the TPCs </span></span></span><span lang="EN-ZA"><span style="line-height:200%"><span new="" roman="" times="">and </span></span></span><span style="background:white"><span style="line-height:200%"><span new="" roman="" times="">TFCs, respectively.</span></span></span></span></span><br> <span style="line-height:200%"><span sans-serif=""><b><span style="background:white"><span style="line-height:200%"><span new="" roman="" times="">Results:</span></span></span></b> <span style="line-height:200%"><span new="" roman="" times="">The percentage of DPPH radical scavenging potential of various leaf and stem-bark extracts and positive control (ascorbic acid) were determined as 6.06-67.13, 5.53-73.70, and 36.08-95.13%, respectively at a concentration range of 93.75-3000&micro;g/mL. The acetone extract from leaves and the dichloromethane extract from stem-bark showed relatively higher scavenging potential. However, all extracts showed relatively lower scavenging potential compared to the positive control. Additionally, the IC₅₀values of leaf and stem-bark extracts were determined in the ranges of 445.82- >3000&micro;g/mL and 612.35- >3000&micro;g/mL, respectively. The IC₅₀value of the positive control was determined as 138.91&micro;g/mL. The acetone leaf extract and <i>n</i>-hexane stem-bark extract showed relatively lower IC₅₀values. The TPCs of various extracts from the leaves and stem-bark were estimated as in the ranges of 0.036-124.702 and 10.417-121.194mg GAE/g DW, respectively</span></span><span lang="EN-ZA"><span style="line-height:200%"><span new="" roman="" times="">. </span></span></span><span style="line-height:200%"><span new="" roman="" times="">The TFCs of various extracts from the leaves and stem-bark were estimated as in the ranges of 4.181-32.776 and 0.235-34.451mg QE/g DW, respectively.</span></span></span></span><br> <span style="line-height:200%"><span sans-serif=""><b><span style="line-height:200%"><span new="" roman="" times="">Conclusion:</span></span></b><span style="line-height:200%"><span new="" roman="" times=""> From this study, we concluded that various </span></span><span lang="EN-GB"><span style="line-height:200%"><span new="" roman="" times="">extracts from the leaves and stem-bark of </span></span></span><i><span style="line-height:200%"><span new="" roman="" times="">C. spicata </span></span></i><span style="line-height:200%"><span new="" roman="" times="">exhibited a </span></span><span lang="EN-GB"><span style="line-height:200%"><span new="" roman="" times="">moderate to significant scavenging potential and </span></span></span><span style="line-height:200%"><span new="" roman="" times="">possessed a moderate to significant TPCs and TFCs. The plant may serve as a potential source of bioactive antioxidant compounds pending further phytochemical and pharmacological investigations.<br> <img alt="" src="./files/site1/images/copuediting.png" style="width: 586px; height: 265px;" ></span></span><span style="line-height:200%"><span new="" roman="" times=""><span style="color:red"></span></span></span></span></span><br> &nbsp;</span></span> Cussonia spicata,Araliaceae,antioxidant activity,DPPH radical scavenging assay,IC50 values,total phenolic contents (TPCs) 0 0 http://pbp.medilam.ac.ir/browse.php?a_code=A-10-1517-2&slc_lang=en&sid=1 Manoharan Karuppiah Pillai kmharan@rediffmail.com 10031947532846006634 10031947532846006634 No University of Eswatini Linda Ndzinisa Ndabenhle 161756@uneswa.ac.sz 10031947532846006635 10031947532846006635 No University of Eswatini Saul Mcelwa Mamba smmamba@uniswa.sz 10031947532846006636 10031947532846006636 No University of Eswatini Vuzumuzi Sifiso Vilane vusvilane@gmail.com 10031947532846006637 10031947532846006637 No University of Eswatini Gomes Dumsani Crispin dgomes@uneswa.ac.sz 10031947532846006638 10031947532846006638 No University of Eswatini Justice Mandlenkhosi Thwala jmthwala@uniswa.sz 10031947532846006639 10031947532846006639 Yes University of Eswatini