Plant Biotechnology Persa

en Synergistic Anticancer Effects of Capecitabine and Naringin on HER2+ (SK-BR-3) and HER2− (MCF-7) Breast Cancer Cell Lines Herbal Drugs پژوهشي Research Objective: Breast cancer is one of the most common malignancies among women, and natural compounds are increasingly investigated as adjuncts to conventional chemotherapy. This study evaluated the combined antiproliferative and pro-apoptotic effects of naringin and capecitabine in MCF-7 and SK-BR-3 breast cancer cell lines. Methods: Cell viability was assessed by MTT assay after treatment with each compound alone and in paired combinations, and drug interactions were explored using the Combination Index (CI). Bax, Bcl-2, and Caspase-3 expression was measured by quantitative real-time PCR. Results: Curve-extrapolated IC50 estimates of naringin were 58 and 56.65 µg/mL in MCF-7 and SK-BR-3 cells, respectively, while the corresponding capecitabine estimates were 619.36 and 679.51 µg/mL. Paired treatments reduced cell viability in a concentration-dependent manner. CI values were below 1 at all four tested combinations in MCF-7 cells and at three of four combinations in SK-BR-3 cells; the lowest-dose combination in SK-BR-3 yielded a CI of 1.437. Because only a limited number of paired concentrations were tested, these results were interpreted as exploratory evidence of enhanced drug interaction rather than definitive pharmacological synergy. Conclusion: Combination treatment increased the Bax/Bcl-2 expression ratio and produced a numerically greater apoptotic response than individual treatments. Antiproliferative activity was greater in SK-BR-3 cells under the tested conditions, but this difference cannot be attributed specifically to HER2 status. Overall, naringin may enhance capecitabine activity, warranting confirmation using broader dose-response matrices and mechanistic studies. <img alt="" src="./files/site1/images/copuediting.png" style="width: 586px; height: 265px;" > ,Naringin,Capecitabine,Breast cancer,MCF7,SK-BR-3,Apoptosis 0 0 http://pbp.medilam.ac.ir/browse.php?a_code=A-10-1578-1&slc_lang=en&sid=1 Sanli Soltannezhad sanli.soltannejad@iau.ac.ir 10031947532846006628 0009-0007-6006-6086 No Department of Biochemistry, NT.C., Islamic Azad University, Tehran, Iran Laya Takbiri Osgoei l.takbiri@iau.ac.ir 10031947532846006629 10031947532846006629 Yes Department of Biochemistry, NT.C., Islamic Azad University, Tehran, Iran Fatemeh Javani Jouni Javani@iau.ac.ir 10031947532846006630 10031947532846006630 No 2 Laser Application in Medical Sciences Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran3 Department of Biochemistry and Biophysics, TeMS.C., Islamic Azad University, Tehran, Iran