fa پژوهشي Research In this study, from June 2019 to May 2019 aerial parts of <em>Scrophularia striata</em> Boiss. were collected from Ilam city and those of <em>Scrophularia deserti</em> Delile from Dehloran city, Ilam province in western Iran. The aerial parts of the medicinal plants were pulverized. The essential and volatile oils were extracted by headspace solid-phase microextraction (HS-SPME) and their chemical compounds analyzed by Gas chromatography&ndash;mass spectrometry (GC-MS). Using HS-SPME, 68 and 49 chemical compounds were identified from <em>S</em>. <em>striata</em> and <em>S</em>. <em>deserti</em>, respectively. Spathulenol (18.41%) was the highest chemical compound of the essential oil of <em>S</em>. <em>striata</em>, followed by caryophyllene oxide (15.38%), linalool (11.85%), alpha-terpineol (8.34%), trans-caryophyllene (4.44%) and geraniol (3.10%). The most important identified compounds from <em>S</em>. <em>deserti</em> were caryophyllene oxide (15.38%), linalool (11.85%), alpha-terpineol (8.34%), trans-caryophyllene (4.44%) and geraniol (3.10%). The most important chemical compounds of <em>S</em>. <em>deserti </em>essential oil included alpha-pinene (25.54%), alpha-phellandrene (19.60%), beta-myrcene (11.29%) and trans-caryophyllene (6.78%). Results show major constituents of this two plant include with a high amount of Spathulenol and caryophyllene oxide has high pharmaceutical and healthful value. Scrophularia striata Boiss., Scrophularia deserti Delile, Essential oil, HS-SPME, GC-MS 8 13 http://pbp.medilam.ac.ir/browse.php?a_code=A-10-25-9&slc_lang=fa&sid=1 Bahmani Mahmoud mahmood.bahmani@gmail.com 10031947532846001045 10031947532846001045 Yes Biotechnology and Medicnal Plants Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran Marzieh Hadavi hadavi.marzieh@ymail.com 10031947532846001046 10031947532846001046 No Department of Internal Medicine, Ilam University of Medical Sciences, Ilam, Iran Naser Abbasi ilamfarma@gmail.com 10031947532846001047 10031947532846001047 No Biotechnology and Medicnal Plants Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran