en Bioactive Compounds پژوهشي Research <span style="font-size:10pt"><span style="line-height:115%"><span style="font-family:&quot;Optima&quot;,serif"><span style="color:black"><b><span style="font-size:9.0pt"><span style="line-height:115%"><span style="font-family:&quot;Times New Roman&quot;,serif">Objective:</span></span></span></b><span style="font-size:9.0pt"><span style="line-height:115%"><span style="font-family:&quot;Times New Roman&quot;,serif"> <i>Vigna unguiculatata (L.)</i> (Cowpea) is an African indigenous protein-dense crop that most sub-Saharan Africans rely on for food and nutrition security.</span></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:115%"><span style="font-family:&quot;Optima&quot;,serif"><span style="color:black"><b><span style="font-size:9.0pt"><span style="line-height:115%"><span style="font-family:&quot;Times New Roman&quot;,serif">Material and Methods:</span></span></span></b><span style="font-size:9.0pt"><span style="line-height:115%"><span style="font-family:&quot;Times New Roman&quot;,serif"> The current study assessed the effect of different levels of poultry manure fertilizer (10, 30, 60 and 90 kg F/ha) and nitrogen fertilizer (30, 45, 60 and 90 kg N/ha) on the growth, yield, mineral composition, bioactive compounds and secondary metabolites in the legume crop <i>Vigna unguiculata</i> (L.). At the end of 15 weeks, 90 kg F/ha and 45 kg N/ha enhanced the growth, yield, mineral composition, production of ascorbic acid, total phenolic and FRAP assay, this informed our decision for the selection of this treatments (45kgN/ha, 90kgF/ha and 0kg/ha control) for further analyses of secondary metabolites.</span></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:115%"><span style="font-family:&quot;Optima&quot;,serif"><span style="color:black"><b><span style="font-size:9.0pt"><span style="line-height:115%"><span style="font-family:&quot;Times New Roman&quot;,serif">Results:</span></span></span></b><span style="font-size:9.0pt"><span style="line-height:115%"><span style="font-family:&quot;Times New Roman&quot;,serif"> Poultry manure and nitrogen fertilizer improved the mineral compositions of cowpea leaves, and different accumulation trends were noted depending on different application levels, with poultry manure responding well.</span></span></span></span></span></span></span><br> <b><span style="font-size:9.0pt"><span style="line-height:107%"><span style="font-family:&quot;Times New Roman&quot;,serif">Conclusion:</span></span></span></b><span style="font-size:9.0pt"><span style="line-height:107%"><span style="font-family:&quot;Times New Roman&quot;,serif"> Thus, the application of organic poultry manure at 90 kg F/ha for cowpea cultivation should potentially be recommended in the Mpumalanga Province.</span></span></span> ,Cowpea,organic fertilizer,FRAP,Mpumalanga Province 35 48 http://pbp.medilam.ac.ir/browse.php?a_code=A-10-428-2&slc_lang=en&sid=1 Ngwatshipane Madonna Mashabela ngwatsipanemashabela@gmail.com 10031947532846002117 0000000233487092 No School of Biology and Environmental Sciences, Faculty of Agriculture and Natural Sciences, University of Mpumalanga,P/Bag X11283, Mbombela, 1200, South Africa Wilfred Otang-Mbeng Wilfred.Mbeng@ump.ac.za 10031947532846002118 0000000257056449 Yes School of Biology and Environmental Sciences, Faculty of Agriculture and Natural Sciences, University of Mpumalanga,P/Bag X11283, Mbombela, 1200, South Africa