Volume 5, Issue 2 (12-2023)                   pbp 2023, 5(2): 33-38 | Back to browse issues page

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Zargoush-Nasab A, Karimi E, Jalilian A, Bahmani M. Quantitative Analysis of Ethanol and Methanol in Herbal Distillates Distributed in Ilam City in Western Iran Using Gas Chromatography. pbp 2023; 5 (2) :33-38
URL: http://pbp.medilam.ac.ir/article-1-197-en.html
1- Biotechnology and Medicinal Plants Research Center, Ilam University of Medical Sciences, Ilam, Iran
2- ssistant Professor of Obstetrics and Gynecology School of Medicine Ilam University of Medical Sciences, Ilam, Iran
3- Biotechnology and Medicinal Plants Research Center, Ilam University of Medical Sciences, Ilam, Iran , mahmood.bahmani@gmail.com
Abstract:   (940 Views)
Background: In this study, five types of popular herbal distillates including licorice, fennel, orange blossom, lavender, and cinnamon were purchased from the supply level stores in Ilam city by cluster sampling method and analyzed for the amount of ethanol and methanol.
Materials and Methods: In the first step, the samples of herbal distillates were taken for testing. In addition, analytical grade ethanol and methanol were purchased from Merck Company and used as controls. Dates of production and expiry of each sample were separately checked and recorded. The samples of every herb distillate were kept in their original containers and used for testing. The names and brands of the manufacturing plants were mentioned as A, B, C, etc., in view of the ethical aspects of the research. The concentration of methanol and ethanol in the products of each company was analyzed by gas chromatography (GC) and each sample was measured five times and the mean value was reported.
Results: On the basis of the obtained results, the mean concentrations of ethanol and methanol in lavender distillate were 7.20 and 17.00 ppm, respectively. The mean ethanol and methanol levels in orange blossom distillate were 164.26 and 64.25 ppm, respectively. The average ethanol and methanol content in cinnamon distillate were 11.95 and 12.77 ppm, respectively. The mean ethanol and methanol contents in fennel distillate were 3.89 and 7.51 ppm, respectively. The mean ethanol and methanol contents in licorice distillate were 4.15 and 38.31 ppm, respectively. The results of this study showed that herbal distillates have ethanols and particularly methanols.
Conclusion: Herbal distillates that contain methanol and ethanol can be toxic, especially to pregnant women, if taken over a long period of time.
Full-Text [PDF 1716 kb]   (90 Downloads)    
Type of Study: Research | Subject: Biotechnology
Received: 2023/04/21 | Accepted: 2023/06/20 | Published: 2023/12/1

References
1. Aienechi A, Pharmacognosy and Iranian Medicinal Plants. Tehran University Publication. IRAN. 1986, pp: 1091 - 8.
2. Fadai Raini, Raha, Enayat Gholampour, Tayyaba, Sepahi, Javad. Effects of lavender medicinal plant (Lavandula angostifolia) on growth performance, survival rate and immune system in Amur fish (Ctenopharyngodon idella). Aquatic nutrition, 2019; 6(2): 1-12. doi: 10.22124/janb.2021.18059.1112
3. Zangeneh M M, Zangeneh A, Etemadi N, Tabatabaei Aghda S J, Pourkamalzadeh M, Zand S, et al . The Effect of Nano Silver Particles of the Aqueous Extract of Artemisia aucheri on the Healing Process of Skin Wounds in Rats. Journal title 2023; 5 (1):1-7. URL: http://pbp.medilam.ac.ir/article-1-189-en.html
4. Ebrahimi, Y., Abdalkareem Jasim, S., Dameh, M., A. Salman, N., Mahdi, O. A., M. Hameed, N., Goudarzi, M. A., Parsaei, P. Determination of Antioxidant Properties of Mentha longifolia, Pistacia khinjuk and Eucalyptus globulus. Caspian Journal of Environmental Sciences, 2022; (): 1-6. doi: 10.22124/cjes.2022.6065
5. Shahsavari S, Sarkar S, Sen DJ, Mandal SK. Determination of total antioxidant activity of methanolic extract of Falcaria vulgaris. Journal of Biochemicals and Phytomedicine. 2022; 1(1): 8–12. doi: 10.34172/jbp.2022.3.
6. Zargari A. Medicinal plants. Tehran University of Medical Sciences; 1997.
7. Sotoudeh, Ibrahim, Mardani, Fatemeh, Jafari, Marzieh, Habibi, Hassan, Moradian, Seyed Hossein. Growth indicators, feeding efficiency and survival of rainbow trout (Oncorhynchus mykiss) fry fed with different levels of Echinacea angustifolia and Origanum majorana extracts. Aquatic nutrition, 2017; 4(1): 1-12. doi: 10.22124/janb.2018.3157
8. Adeleke BS, Babalola OO. Pharmacological potential of fungal endophytes associated with medicinal plants: A review. Journal of Fungi. 2021 Feb 17;7(2):147.
9. Khoram Z, Naine A, Rafieinezha R, Hakimaneh SM, Hakimaneh SM, Shayehg SS, Salari AM. The Antifungal Effects of Two Herbal Essences in Comparison with Nystatin on the Candida Strains Isolated from the Edentulous Patients. Journal of Contemporary Dental Practice. 2019 Jun 21;20(6).
10. Sefidkon F. Production of standard herbal essences, the necessity of the day for the country's medicinal plants. Iran Nature. 2021 Jul 23;6(3):143-.
11. Karimi, Gh., Hassanzadeh, M., Shahidi, N & Samiei, Z. (2007). "Methanol determinehion in herbal distillates produeed with spectro photometry method in Mashhad. GilanBaroo7(1): 759-77.
12. Brent, J., Wallace, KL., Burkhart, KK., Phillips, SD& Donovan, JW. (2005). Diagnosis and management of the critically poisoned patient. Critical care toxicology, pp. 441-447.
13. Adzet, T., Ponz, R., Wolf, E & Schulte, E. (1992). Content and composition of M. officinalis oil in relation to leaf position and harvest time1. Planta medica, 58(06), 562- 564.
14. Ford M, Delaney K, Ling L and Erickson T. Clinical Toxicology. WB Saunders Company. USA. 2001, pp: 759 - 67.
15. Brent J, Wallace K, Burkhart K, Phillip S and Ward Donovan. J. Critical Care Toxicology. Elsevier Mosby. USA. 2005, pp: 895 - 907.
16. Taghaddossnejad F. Clinical Toxicology and Poisoning Diagnosis and Treatment. 1985; pp. 164-167.
17. Solhi H, Delavar M, Cheshm Jahanbin A, Abdollahi M. Comparison of methanol concentration in handmade herbal essences produced in Arak city with industrial produced herbal essences with different commercial brands. Arak Medical University Journal 2009; 12(3): 85-91.
18. Rafizadeh A, Shariati SH, Pourmohammad L, Fooladmehr S. Application a colorimetric method for qualitative analysis of methanol. Scin J Forensic Med 2010; 16: 94-89.
19. Rafizadeh A, Pourmohammad L, Shariati SH, Mirzajani E. Introduce of a colorimetric method for qualitative detection of methanol in several kinds of drinks. Journal of Mazandaran Medical Science 2011; 21: 150-152.
20. Rafizadeh A, Nasiri Fard R, Nasoori Gazni M, Haghshnace M, Jmali Biverzani F, Pourmohammad L. The effectiveness of whole Concentration of homemade herbal distillates on the result of qualitative methanol detection by the chromotropic acid method. Journal of Ornamental and Horticultural Plants 2013; 3: 105-109.
21. Rafizadeh A, Shariati S, Safarzadeh Vishekaei M. Determination of Herbal Distillates Methanol Using a New Diagnostic Kit. Journal title 2016; 24 (96) :61-67
22. URL: http://journal.gums.ac.ir/article-1-1102-fa.html
23. Nemecek-Marshall M, MacDonald RC, Franzen JJ, Wojciechowski CL, Fall R. Methanol emission from leaves: enzymatic detection of gas-phase methanol and relation of methanol fluxes to stomata conductance and leaf development. Plant Physiol 1995; 108: 1359–1368
24. Hemming D, Criddle R. Effects of methanol on plant respiration. J Plant Physiol 1995; 146: 193–198
25. Madani, Fatemeh and Hosseini, Fakhri Sadat and Shafqat, Ali, 2013, investigation of methanol in dill sweat produced by industrial method, the second national conference of medicinal plants and sustainable agriculture, Hamedan, https://civilica.com/doc/ 306074
26. Paine A, Davan AD. Defining a tolerable concentration of methanol in alcoholic drinks. Hum Exp Toxicol. 2007;20:563–568.
27. Szucs S, Sarvary A, McKee M, Adany R. Could the high level of cirrhosis in Central and Eastern Europe be due partly to the quality of alcohol consumed. Addiction. 2005; 100:536–42.
28. Laats MM, Grosdenis F, Recourt K, Voragen AGJ, Wichers HJ. Partial purification and characterization of pectin methylesterase from green beans (Phaseolus Vulgaris L.) J Agric Food Chem. 1997;45:572–577.
29. Anthon GE, Barrett DM. Characterization of the temperature activation of pectin methylesterase in green beans and tomatoes. J Agric Food Chem. 2006;54:204–211.
30. Cabaroglu T. Methanol contents of Turkish varietal wines and effect of processing. Food Control. 2005;16:177–181.
31. Bouchard M, Droz PO, Carrier G. A iologically based dynamic model for predicting the disposition of methanol and its metabolites in animals and humans. Toxicol Sci. 2001;64:169–184.
32. Hemming D, Criddle R. Effects of methanol on plant respiration. J Plant Physiol 1995; 146: 193–198

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