Volume 7, Issue 2 (5-2025)
pbp 2025, 7(2): 111-118 |
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Etemadi N, Froushani S M A, Zangeneh A, Zangeneh M M, Pirnejad-Talatapeh A, Abangah G, et al . The effect of silver nanoparticles of aqueous extract of Artemisia dracunculus on the prevention of gastric ulcer induced by ethanol in rats. pbp 2025; 7 (2) :111-118
URL: http://pbp.medilam.ac.ir/article-1-202-en.html
URL: http://pbp.medilam.ac.ir/article-1-202-en.html
Navid Etemadi *1 
, Seyyed Meysam Abtahi Froushani2 , Akram Zangeneh3 
, Mohammad Mehdi Zangeneh3 , Ali Pirnejad-Talatapeh4 , Ghobad Abangah5 , Siavosh Kaki Sahne6 , Shahin Ehteshamfar7
, Seyyed Meysam Abtahi Froushani2 , Akram Zangeneh3 , Mohammad Mehdi Zangeneh3 , Ali Pirnejad-Talatapeh4 , Ghobad Abangah5 , Siavosh Kaki Sahne6 , Shahin Ehteshamfar7
1- DVM Graduate, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran , Navidetemadi74@gmail.com
2- Department of Microbiology, Faculty of veterinary Medicine, Urmia University, Urmia, Iran
3- Biotechnology and Medicinal plants Research Center Ilam University of Medical Sciences, Ilam, Iran
4- Central Laboratory, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
5- School of Medicine, Shahid Mostafa Khomaeini Hospital, Ilam University of Medical Sciences, Ilam, Iran
6- DVM Student, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
7- Department of Internal Medicine and Clinical Pathology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
2- Department of Microbiology, Faculty of veterinary Medicine, Urmia University, Urmia, Iran
3- Biotechnology and Medicinal plants Research Center Ilam University of Medical Sciences, Ilam, Iran
4- Central Laboratory, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
5- School of Medicine, Shahid Mostafa Khomaeini Hospital, Ilam University of Medical Sciences, Ilam, Iran
6- DVM Student, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
7- Department of Internal Medicine and Clinical Pathology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Abstract: (1671 Views)
Objective: One of the common diseases among the communities is stomach ulcer. Common treatments for this disease include drugs that reduce acid secretion, which, while effective, have side effects. The use of medicinal plants as an aid in the treatment of this disease is considered. One of the application fields of nanobiotechnology is the use of silver nanoparticles (Nanosilver particles) for a new solution in the treatment of digestive diseases. This study was conducted to determine the effect of silver nanoparticles combined with Artemisia dracunculus extract for the prevention of ethanol-induced gastric ulcer.
Method: To conduct this study, 28 rats (220-250 grams) were used in 4 experimental groups. The experimental groups included the saline group, the groups receiving aqueous Artemisia dracunculus extract with a dose of 1000 mg/kg and silver nanoparticles of Artemisia dracunculus aqueous extract with a dose of 0.5 mg/kg, the omeprazole group with a dose of 20 mg/kg orally, and the negative control group. 12 days after the treatment, gastric ulcer was induced by feeding half a cc of pure ethanol to each animal. After 4 hours, the animals were anesthetized, blood was drawn from the heart, and the stomach tissue was separated and used for histopathological and biochemical studies. The amount of superoxide dismutase (SOD) in serum and the amount of malondialdehyde (MDA) in stomach tissue were determined.
Results and Discussion: The results in the tested groups show a decrease in the ulcer index and an increase in the percentage of wound inhibition and the protective effect of silver nano particles of the Artemisia dracunculus extract. The significant increase in the amount of SOD and the significant decrease in MDA due to the administration of nanoparticles of Artemisia dracunculus extract and omeprazole indicate the beneficial effect of the silver nanoparticle of the Artemisia dracunculus extract based on its antioxidant effects (reduction of oxidative stress).
Method: To conduct this study, 28 rats (220-250 grams) were used in 4 experimental groups. The experimental groups included the saline group, the groups receiving aqueous Artemisia dracunculus extract with a dose of 1000 mg/kg and silver nanoparticles of Artemisia dracunculus aqueous extract with a dose of 0.5 mg/kg, the omeprazole group with a dose of 20 mg/kg orally, and the negative control group. 12 days after the treatment, gastric ulcer was induced by feeding half a cc of pure ethanol to each animal. After 4 hours, the animals were anesthetized, blood was drawn from the heart, and the stomach tissue was separated and used for histopathological and biochemical studies. The amount of superoxide dismutase (SOD) in serum and the amount of malondialdehyde (MDA) in stomach tissue were determined.
Results and Discussion: The results in the tested groups show a decrease in the ulcer index and an increase in the percentage of wound inhibition and the protective effect of silver nano particles of the Artemisia dracunculus extract. The significant increase in the amount of SOD and the significant decrease in MDA due to the administration of nanoparticles of Artemisia dracunculus extract and omeprazole indicate the beneficial effect of the silver nanoparticle of the Artemisia dracunculus extract based on its antioxidant effects (reduction of oxidative stress).
Received: 2023/10/31 | Accepted: 2023/12/1 | Published: 2023/12/1
References
1. Allahtavakoli M, Khaksari Haddad M, Assar Sh. Comparison of topical application of Mummify and Phenytoin cream on skin wound healing in rat. J Babol Univ Med Sci 2003;5(18):7-13.DOR 20.1001.1.15614107.1382.5.2.1.6
2. Asharani PV, Wu YL, Gong Z, Valiyaveettil S. Toxicity of silver nanoparticles in zebrafish models. Nanotechnology 2008; 19(25): 255102. DOI: 10.1088/0957-4484/19/25/255102
3. B.S. Nayak, G. Isitor, E.M. Davis, G.K. Pillai, The evidence based wound healing activity of Lawsonia inermis Linn, Phytother. Res. 21 (2007) 827–831. DOI: 10.1002/ptr.2181
4. D. Dwivedi, M. Dwivedi, S. Malviya, V. Singh, Evaluation ofwound healing, antimicrobial and antioxidant potential of Pongamia pinnata in wistar rats, J. Tradit. Complement. Med. 7 (2017) 79–85. DOI: 10.1016/j.jtcme.2015.12.002
5. Dinani NJ, Asgary A, Madani H, Naderi G, Mahzoni P. Hypocholesterolemic and antiatherosclerotic effect of artemisia aucheri in hypercholesterolemic rabbits. Pak J Pharm m Sci 2010; 23(3): 321-325.
6. Freunscht P, Van Duyne RP, Schneider S. Surface-enhanced Raman spectroscopy of trans-stilbene adsorbed on platinum- or self-assembled monolayer-modified silver film over nanosphere surfaces. Chemical Physics Letters. 1997; 281(4): 372-8.
7. G.D. Phillips, R.A. Whitehe, D.R. Kinghton, Initiation and pattern of angiogenesis in wound healing in the rats, Am. J. Anat. 192 (1991) 257–262. DOI: 10.1002/aja.1001920305
8. G.F. Caetano, M. Fronza, M.N. Leite, A. Gomes, M.A.C. Frade, Comparison of collagen content in skin wounds evaluated by biochemical assay and by computeraided histomorphometric analysis, Pharm. Biol. 64 (2016) 2555–2559. DOI: 10.3109/13880209.2016.1170861
9. G.S. Lazarus, D.M. Cooper, D.R. Knighton, D.J. Margolis, R.E. Pecoraro, G. Rodeheaver, M.C. Robson, Definitions and guidelines for assessment of wounds and evaluation of healing, Arch. Dermatol. 130 (1994) 489–493.
10. H. Azhdari-Zarmehri, S. Nazemi, E. Ghasemi, Z. Musavi, Z. Tahmasebi, F. Farsad, A. Farzam, Assessment of effect of hydro-alcoholic extract of scrophularia striata on burn healing in rat, JBUMS. 16 (2014) 42–48. DO.10.1007/s00580-019-02988-x
11. indices of wound healing in chronic diabetic rat. J Babol Univ Med Sci 2005; 8(29): 12-21.
12. Jarrahi M, Emami Abarghuee M. Effect of Hydro-Alcoholic extract of Matricaria chamomilla L on burned wound healing in rat. J Gorgan Uni Med Sci 2008; 10(26): 22-26.
13. Jarrahi M, Zahedi M, Ajorlu M. Effect of Aloe barbadensis Miller on skin wound healing in rat. J Gorgan Uni Med Sci 2009; 11(29): 13-17.
14. Johnston DE. Wound healing in skin, plastic and reconstructive surgery. Vet Clinic North Am. 1990; 20:1-45.
15. Khaksari M, Mardani M, Rezaee zadeh A. Effect of oral ascorbic acid on histological.
16. Mansour Ghanaie F, Sigaroudi S, Mobasheri HR, Jallili A. Effect of Artemisia on Asthma. Faiz 2003; 7(25): 60-63.
17. Mousavi ZB, Meshki M, Hemmati A, Veisi MS, Rafiei R. Evaluation of the efficacy of Artemisia Aucheri on
18. Healing of Skin Wound in Rat. J Mazand Univ Med Sci 2010; 20(77): 70-76 (Persian)
19. Park EJ, Yi J, Kim Y, Choi K, Park K. Silver nanoparticles induce cytotoxicity by a Trojan-horse type mechanism. Toxicol In Vitro 2010; 24: 872–8. DOI: 10.1016/j.tiv.2009.12.001
20. Quince mucilage on wound healing. Iranian J Dermatology 2006; 9(37): 260-263.
21. Rezvani ME, Roohbakhsh A, Allahtavakoli M, Shamsizadeh A. Anticonvulsant effect of aqueous extract of Valeriana officinalis in amygdala-kindled rats: possible involvement of adenosine. J Ethnopharmacol 2010; 127(2): 313-318. DOI: 10.1016/j.jep.2009.11.002
22. Sadeghi Fard H, Zareian P. Survey on analgesic effect of Hydro-alcoholic extract of Artemisia aucheri in two models of acute and chronic pain. J Kordestan Univ Med Sci 2008; 13(4): 30-36.
23. Said Fernández S, Ramos Guerra MC, Mata Cárdenas BD, Vargas Villarreal J, Villarreal Treviño L. In vitro antiprotozoal activity of the leaves of Artemisia ludoviciana. Fitoterapia 2005; 76(5): 466-468. DOI: 10.1016/j.fitote.2005.04.009
24. Sarkar S, Jana AD, Samanta SK, Mostafa G. Facile synthesis of silver nano particles with highly efficient anti-microbial property. Polyhedron. 2007; 26: 4419-26.
25. Sewall GK, Robertson KM, Connor NP, Heisey DM, Hartig GK. Effect of topical mitomycin on skin wound contraction. Arch Facial Plast Surg. 2003;5:59-62. doi.org/10.1001/archfaci.5.1.59
26. Souba WW, Wilmore D. Diet and nutrition in case of the patient with surgery. 9th ed, Baltimore:
27. Takenaka S, Karg E, Roth C, Schulz H, Ziesenis A, Heinzmann U, et al. Pulmonary and systemic distribution of inhaled ultrafine silver particles in rats. Environ Health Perspect. 2001 Aug; 109(4):547-51. DOI: 10.1289/ehp.01109s4547
28. Townsend CM. Sabiston textbook of surgery. 16th ed, New York: Oxford University Prass; 2001:85-91.
29. Tsuda K, Nakatani T, Sugama J, Okuwa M, Sanada H. Influence of the timing of switching a protein-free to a protein-containing diet on the wound healing process in a rat all-layer skin defect. Int Wound J 2010; 7(3): 135-146. DOI: 10.1111/j.1742-481X.2010.00674.x
30. G.D. Phillips, R.A. Whitehe, D.R. Kinghton, Initiation and pattern of angiogenesis in wound healing in the rats, Am. J. Anat. 192 (1991) 257–262.DOI: 10.1002/aja.1001920305
31. Yoshimaru T, Suzuki Y, Inoue T, Niide O, Ra C. Silver activates mast cells through reactive oxygen species production and a thiol-sensitive store-independent Cainflux. Free Radic Biol Med 2006; 40: 1949–59. DOI: 10.1016/j.freeradbiomed.2006.01.023
32. Zareian P, Zahiri Sh, Ketabchi F, Ruzmeh Sh. Effect of local Tamarix monnifera on skin wound healing process in rabbit. J Mazand Univ Med Sci 2007; 17(57):4857..
33. Zargari A. Herbal plants. 6th ed, Tehran: Tehran University Publisher; 1999. P 63. DOI: 10.4236/ajibm.2016.64043
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