Evaluation of Antibacterial Effects of Newly Synthesized Derivative Methyl 2'-Methyl-1,3-Dioxo-1,1',2',3,5',6',7',7a'-Octahydrospiro[Indene-2,3'-Pyrrolizidine]-2' Carboxylate against Staphylococus Aureus and Escherichia Coli.

Akbari, Reza; azarifam, safa; kouhkan, mehri

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Akbari R, azarifam S, kouhkan M. Evaluation of Antibacterial Effects of Newly Synthesized Derivative Methyl 2'-Methyl-1,3-Dioxo-1,1',2',3,5',6',7',7a'-Octahydrospiro[Indene-2,3'-Pyrrolizidine]-2' Carboxylate against Staphylococus Aureus and Escherichia Coli.. pbp 2024; 6 (1)
URL: http://pbp.medilam.ac.ir/article-1-212-en.html

Evaluation of Antibacterial Effects of Newly Synthesized Derivative Methyl 2'-Methyl-1,3-Dioxo-1,1',2',3,5',6',7',7a'-Octahydrospiro[Indene-2,3'-Pyrrolizidine]-2' Carboxylate against Staphylococus Aureus and Escherichia Coli.

Reza Akbari¹

, Safa Azarifam²

, Mehri Kouhkan ^*

1- Department of Microbiology and Virology, School of Medicine, Urmia University of MedicalSciences, Urmia, Iran
2- Faculty of Pharmacy, Urmia University of Medical Sciences, Urmia, Iran
3- Department of Medicinal Chemistry, School of Pharmacy, Urmia University of Medical Sciences,Urmia, Iran , mehrikouhkan@gmail.com

Abstract: (243 Views)

Unfortunately, infectious diseases are still one of the most important health problems around the world. The resistance of pathogenic bacteria to several drugs is an important and growing problem in the treatment of infectious diseases and hospital infections. It seems necessary to identify new compounds that have inhibitory or lethal effects on this bacterium. That's why we decided antimicrobial efficacy. The newly synthesized compound (2-methyl-1,3-dioxo-',11',2',3',5',6'7',a7-octahydrospiro]inden-'2 3-pyrrolizidine 2) and 2-carboxylate (6) on Staphylococcus aureus and Escherichia coli bacteria. At first, synthesized of methyl'2-methyl-1,3-dioxo-1',1',2',3',5',6',7'a7-octahydrospiro]inden-'2,3-pyrrolizidine 2- Carboxylate was prepared by green chemistry method and with the help of microwave in one pot. Microbial culture media containing control bacteria of Staphylococcus aureus and Escherichia coli were prepared and concentrations of 70 to 110 Landa of the above compound were added to the samples. The antimicrobial property of the samples was determined after 3 days using the disk diffusion method and broth microdilution method was determined, as well as the minimum inhibitory concentration and bacterial lethality of the samples. The minimum inhibitory concentration and bacterial lethality in the above combination against the same standard strain of Escherichia coli bacteria was obtained at the rate of 12.5 μg/ml. The minimum inhibitory and lethal concentration of methyl methacrylate compound against the standard strain of Staphylococcus aureus bacteria was 12.5 and 25 μg/ml, respectively.The use of the above combination was effective in controlling and inhibiting the tested bacteria.

Keywords: Pyrrolizidine, Staphylococcus aureus and Escherichia coli

Type of Study: Research | Subject: Herbal Drugs
Received: 2023/11/28 | Accepted: 2024/01/18 | Published: 2024/02/14

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