Volume 7, Issue 1 (2-2025)
pbp 2025, 7(1): 101-108 |
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Karimi A, Akbari R, kouhkan M. Assessment of Antibacterial Effects of Ethyle Octahydrospiro[indene-2,3'-pyrrolizidine]- 2' Carboxylate Against Escherichia coli and Staphylococcus aureus bacteria. pbp 2025; 7 (1) :101-108
URL: http://pbp.medilam.ac.ir/article-1-223-en.html
URL: http://pbp.medilam.ac.ir/article-1-223-en.html
1- Student Faculty of Pharmacy, Urmia University of Medical Sciences
2- Department of Microbiology and Virology, School of Medicine, Urmia University of MedicalSciences, Urmia, Iran
3- Department of Medicinal Chemistry, School of Pharmacy, Urmia University of Medical Sciences,Urmia, Iran. ,mehrikouhkan@gmail.com
2- Department of Microbiology and Virology, School of Medicine, Urmia University of MedicalSciences, Urmia, Iran
3- Department of Medicinal Chemistry, School of Pharmacy, Urmia University of Medical Sciences,Urmia, Iran. ,
Abstract: (588 Views)
Objective: The emergence of resistance in bacteria, and the existence of various types of infection and contamination in the hospital and community is the basis for research to find new antibacterial compounds.
Materials: Ethyl Octahydrospiro[indene-2,3'-pyrrolizidine]-1,3-diones-pyrrolizidine]-2'carboxylate (5) was synthesized during one-pot reaction of ninhydrin, proline and ethyl acrylate in ethanol solvent. The antibacterial effect of the synthesized derivative on the mentioned bacteria was investigated by agar plate culture and broth micro dilution methods and the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined for the synthesized derivative.
Results: The analysis of the results showed that the MIC and MBC values of the newly synthesized compound against Staphylococcus aureus (S. aureus) bacteria were 1.56 and 3.12 μg/mlL, respectively. In addition, the MIC and MBC values of the synthetic compound against Escherichia coli (E. coli) were 0.39 μg/mlL and 0.78 μg/mlL, respectively.
Conclusion: The antimicrobial test results revealed that the synthesized compound at an equivalent concentration exhibited a higher inhibitory impact on the Gram-negative E. coli bacteria than Gram-positive S. aureus. The compound causes the rapid bactericidal effect on Gram-negative bacteria by destroying or disrupting the function of the outer membrane. This combination could be effective in treating infections caused by Gram-negative bacteria.
Materials: Ethyl Octahydrospiro[indene-2,3'-pyrrolizidine]-1,3-diones-pyrrolizidine]-2'carboxylate (5) was synthesized during one-pot reaction of ninhydrin, proline and ethyl acrylate in ethanol solvent. The antibacterial effect of the synthesized derivative on the mentioned bacteria was investigated by agar plate culture and broth micro dilution methods and the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined for the synthesized derivative.
Results: The analysis of the results showed that the MIC and MBC values of the newly synthesized compound against Staphylococcus aureus (S. aureus) bacteria were 1.56 and 3.12 μg/mlL, respectively. In addition, the MIC and MBC values of the synthetic compound against Escherichia coli (E. coli) were 0.39 μg/mlL and 0.78 μg/mlL, respectively.
Conclusion: The antimicrobial test results revealed that the synthesized compound at an equivalent concentration exhibited a higher inhibitory impact on the Gram-negative E. coli bacteria than Gram-positive S. aureus. The compound causes the rapid bactericidal effect on Gram-negative bacteria by destroying or disrupting the function of the outer membrane. This combination could be effective in treating infections caused by Gram-negative bacteria.
Type of Study: Research |
Subject:
Green Chemistry
Received: 2024/05/21 | Accepted: 2024/11/27 | Published: 2025/02/28
Received: 2024/05/21 | Accepted: 2024/11/27 | Published: 2025/02/28
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