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Rakhshan K, Marzban A, Haghighatian Z, Raisi A, Zarei L, Roshani A. Evaluation of Zinc Oxide Nanoparticles Coated with Acetylsalicylate on the Wound Healing Process in Male Rats. pbp 2026; 8
URL: http://pbp.medilam.ac.ir/article-1-326-en.html
URL: http://pbp.medilam.ac.ir/article-1-326-en.html
Kazem Rakhshan1 
, Abdolrazagh Marzban2 , Zahra Haghighatian3 , Abbas Raisi4 , Leila Zarei *5 , Asadollah Roshani6
, Abdolrazagh Marzban2 , Zahra Haghighatian3 , Abbas Raisi4 , Leila Zarei *5 , Asadollah Roshani6
1- Student Research Committee, Lorestan University of Medical Sciences, Khorramabad, Iran
2- Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
3- Department of Pathology, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran
4- Department of Clinical Sciences, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran
5- Palliative Care Research Center, Ur.C., Islamic Azad University, Urmia, Iran ,leilazarei652@yahoo.com
6- Department of Surgery, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran
2- Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
3- Department of Pathology, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran
4- Department of Clinical Sciences, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran
5- Palliative Care Research Center, Ur.C., Islamic Azad University, Urmia, Iran ,
6- Department of Surgery, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran
Abstract: (457 Views)
Objective: Wound healing is a vital physiological process that maintains the skin’s barrier function and prevents infection. The emergence of nanotechnology, particularly metallic nanoparticles, has introduced new strategies to enhance and accelerate wound repair. Among these, zinc oxide nanoparticles coated with acetylsalicylate have attracted attention due to their combined antibacterial and regenerative properties, positioning them as a promising therapeutic option in wound management. This study aimed to evaluate the effects of acetylsalicylate-coated zinc oxide nanoparticles on wound healing in male rats, with an emphasis on histopathological outcomes.
Methods: Wound repair involves a complex interplay of cellular and biochemical events leading to the restoration of tissue integrity. Forty-eight healthy male Wistar rats of uniform weight were randomly assigned to four groups (n=12 each) after standardized wound induction: Group I (control, no treatment), Group II (treated with standard phenytoin ointment), Group III (treated with base ointment), and Group IV (treated with acetylsalicylate-coated zinc oxide nanoparticles). Treatments were applied once daily for seven consecutive days. Histopathological assessments were conducted on days 7, 14, and 21 post-wounding.
Results: Group IV demonstrated significantly improved histopathological indices and accelerated wound healing compared with the other groups (P < 0.05). The findings indicate that acetylsalicylate-coated zinc oxide nanoparticles possess potent anti-inflammatory, antibacterial, and tissue-regenerative effects that substantially enhance wound repair in this animal model. Notably, epidermal and dermal regeneration across different healing phases was markedly improved, underscoring the efficacy of this nanocomposite.
Conclusion: Acetylsalicylate-coated zinc oxide nanoparticles may serve as a novel and effective therapeutic modality for wound management, particularly in infected wounds. Nevertheless, additional preclinical studies and clinical trials are required to confirm these effects and support their translation to human clinical applications.
Methods: Wound repair involves a complex interplay of cellular and biochemical events leading to the restoration of tissue integrity. Forty-eight healthy male Wistar rats of uniform weight were randomly assigned to four groups (n=12 each) after standardized wound induction: Group I (control, no treatment), Group II (treated with standard phenytoin ointment), Group III (treated with base ointment), and Group IV (treated with acetylsalicylate-coated zinc oxide nanoparticles). Treatments were applied once daily for seven consecutive days. Histopathological assessments were conducted on days 7, 14, and 21 post-wounding.
Results: Group IV demonstrated significantly improved histopathological indices and accelerated wound healing compared with the other groups (P < 0.05). The findings indicate that acetylsalicylate-coated zinc oxide nanoparticles possess potent anti-inflammatory, antibacterial, and tissue-regenerative effects that substantially enhance wound repair in this animal model. Notably, epidermal and dermal regeneration across different healing phases was markedly improved, underscoring the efficacy of this nanocomposite.
Conclusion: Acetylsalicylate-coated zinc oxide nanoparticles may serve as a novel and effective therapeutic modality for wound management, particularly in infected wounds. Nevertheless, additional preclinical studies and clinical trials are required to confirm these effects and support their translation to human clinical applications.
Type of Study: Research |
Subject:
Clinical
Received: 2025/04/6 | Accepted: 2025/08/6 | Published: 2025/12/1
Received: 2025/04/6 | Accepted: 2025/08/6 | Published: 2025/12/1
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