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Rakhshan K, Marzban A, Haghighatian Z, Raisi A, Zarei L, Roshani A. Evaluation of Zinc Oxide Nanoparticles Coated with Acetylsalicylate on the Wound Healing Process in Male Rats. pbp 2026; 8 (1)
URL: http://pbp.medilam.ac.ir/article-1-326-en.html
URL: http://pbp.medilam.ac.ir/article-1-326-en.html
Kazem Rakhshan1 
, Abdolrazagh Marzban2 , Zahra Haghighatian3 , Abbas Raisi4 , Leila Zarei *5 , Asadollah Roshani6
, Abdolrazagh Marzban2 , Zahra Haghighatian3 , Abbas Raisi4 , Leila Zarei *5 , Asadollah Roshani6
1- Student Research Committee, Lorestan University of Medical Sciences, Khorramabad, Iran
2- Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
3- Department of Pathology, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran
4- Department of Clinical Sciences, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran
5- Palliative Care Research Center, Ur.C., Islamic Azad University, Urmia, Iran ,leilazarei652@yahoo.com
6- Department of Surgery, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran
2- Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
3- Department of Pathology, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran
4- Department of Clinical Sciences, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran
5- Palliative Care Research Center, Ur.C., Islamic Azad University, Urmia, Iran ,
6- Department of Surgery, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran
Abstract: (224 Views)
Objective: Wound healing is a critical physiological process that maintains the skin’s barrier function and prevents infection. The advent of nanotechnology, particularly the use of metallic nanoparticles, has opened new avenues to enhance the quality and accelerate the rate of wound repair. Among these, zinc oxide nanoparticles coated with acetylsalicylate have garnered considerable attention due to their combined antibacterial and regenerative properties, positioning them as a promising therapeutic agent in wound management. This study aimed to investigate the therapeutic effects of acetylsalicylate-coated zinc oxide nanoparticles on wound healing in male rats, with a particular focus on histopathological outcomes.
Methods: Wound repair is a complex interaction of cellular and biochemical events culminating in the restoration of tissue integrity and strength. Forty-eight healthy male Wistar rats of uniform weight were randomly assigned into four groups (n=12 each) following the induction of standardized wounds: Group I (control, no treatment), Group II (treated with standard phenytoin ointment), Group III (treated with base ointment), and Group IV (treated with acetylsalicylate-coated zinc oxide nanoparticles). Treatments were applied once daily for seven consecutive days. Histopathological assessments were performed on days 7, 14, and 21 post-wounding.
Results: The results demonstrated that Group IV exhibited significantly enhanced histopathological indices and wound healing progression compared to the other groups (P < 0.05). These findings suggest that zinc oxide nanoparticles coated with acetylsalicylate exert potent anti-inflammatory, antibacterial, and tissue-regenerative effects that substantially promote wound repair in this animal model. The marked improvement in epidermal and dermal regeneration across different phases of healing highlights the efficacy of this nanocomposite.
Conclusion: Given these promising results, acetylsalicylate-coated zinc oxide nanoparticles may represent a novel and effective therapeutic modality for wound management, particularly for infected wounds. Nonetheless, further preclinical studies and clinical trials are necessary to validate these effects and support their translation to human clinical applications.
Methods: Wound repair is a complex interaction of cellular and biochemical events culminating in the restoration of tissue integrity and strength. Forty-eight healthy male Wistar rats of uniform weight were randomly assigned into four groups (n=12 each) following the induction of standardized wounds: Group I (control, no treatment), Group II (treated with standard phenytoin ointment), Group III (treated with base ointment), and Group IV (treated with acetylsalicylate-coated zinc oxide nanoparticles). Treatments were applied once daily for seven consecutive days. Histopathological assessments were performed on days 7, 14, and 21 post-wounding.
Results: The results demonstrated that Group IV exhibited significantly enhanced histopathological indices and wound healing progression compared to the other groups (P < 0.05). These findings suggest that zinc oxide nanoparticles coated with acetylsalicylate exert potent anti-inflammatory, antibacterial, and tissue-regenerative effects that substantially promote wound repair in this animal model. The marked improvement in epidermal and dermal regeneration across different phases of healing highlights the efficacy of this nanocomposite.
Conclusion: Given these promising results, acetylsalicylate-coated zinc oxide nanoparticles may represent a novel and effective therapeutic modality for wound management, particularly for infected wounds. Nonetheless, further preclinical studies and clinical trials are necessary to validate these effects and support their translation to human clinical applications.
Type of Study: Research |
Subject:
Clinical
Received: 2025/04/6 | Accepted: 2025/08/6 | Published: 2025/12/1
Received: 2025/04/6 | Accepted: 2025/08/6 | Published: 2025/12/1
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