Volume 4, Issue 1 (6-2022)
pbp 2022, 4(1): 10-17 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Fazeli-Nasab B, Shahraki-Mojahed L, Dahmardeh N. Evaluation of Antimicrobial Activity of Essential Oil and Ethanolic Extract of 10 Medicinal Plants on Rathayibacter tritici and Xanthomonas translucens. pbp 2022; 4 (1) :10-17
URL: http://pbp.medilam.ac.ir/article-1-88-en.html
URL: http://pbp.medilam.ac.ir/article-1-88-en.html
1- Research Department of Agronomy and Plant Breeding, Agricultural Research Institute, University of Zabol, Zabol, Iran. , bfazelinasab@gmail.com
2- Department of Biochemistry, School of Medicine, Zabol University of Medical Sciences, Zabol, Iran.
3- Department of Anatomical sciences, Faculty of medicine, Zabol University of medical sciences, Zabol, Iran.
2- Department of Biochemistry, School of Medicine, Zabol University of Medical Sciences, Zabol, Iran.
3- Department of Anatomical sciences, Faculty of medicine, Zabol University of medical sciences, Zabol, Iran.
Abstract: (1904 Views)
Objective: Diseases caused by various drug-resistant strains in plants are increasing in many countries, so many efforts have been made to find new compounds as a suitable alternative to chemical drugs and pesticides. In this study, the antimicrobial effect of essential oils and ethanolic extracts of 10 medicinal plants were investigated on Rathayibacter tritici and Xanthomonas translucens.
Material and Methods: Alcoholic extracts of medicinal plants were extracted using a rotary apparatus. Two standard bacteria R. tritici and X. translucens were prepared from Persian Type Culture Collection. The minimum inhibitory concentration and the minimum inhibitory concentration of essential oils and ethanolic extracts of plants used at a concentration of 50 mg/ ml were determined by dilution in liquid medium on pathogens.
Results: Based on the results, the lowest inhibitory concentration of thyme essential oil was 6.25 ppm, which was inhibited by R. tritici, and the lowest concentration of Hypericum perforatum essential oil against X. translucens was 6.25 ppm. The lowest concentrations of essential oils of yew and fennel were 6.25 ppm, which were inhibited by both bacteria. Rubia tinctorum leaf essential oil in a concentration of 6.25 only inhibited R. tritici bacteria. The antibacterial properties of the essential oils of the studied plants were higher than the extract.
Conclusion: Essential oils of yew and oleander were the most effective against R. tritici and X. translucens, followed by thyme and rosemary against R. tritici and herring flower against X. translucens. Although the clinical use of ethanolic extracts and essential oils of the studied plants seems valuable due to side effects, but for the clinical use of essential oils and extracts, more research should be done on the mechanism of action of effective compounds of these plants on microbial agents.
Material and Methods: Alcoholic extracts of medicinal plants were extracted using a rotary apparatus. Two standard bacteria R. tritici and X. translucens were prepared from Persian Type Culture Collection. The minimum inhibitory concentration and the minimum inhibitory concentration of essential oils and ethanolic extracts of plants used at a concentration of 50 mg/ ml were determined by dilution in liquid medium on pathogens.
Results: Based on the results, the lowest inhibitory concentration of thyme essential oil was 6.25 ppm, which was inhibited by R. tritici, and the lowest concentration of Hypericum perforatum essential oil against X. translucens was 6.25 ppm. The lowest concentrations of essential oils of yew and fennel were 6.25 ppm, which were inhibited by both bacteria. Rubia tinctorum leaf essential oil in a concentration of 6.25 only inhibited R. tritici bacteria. The antibacterial properties of the essential oils of the studied plants were higher than the extract.
Conclusion: Essential oils of yew and oleander were the most effective against R. tritici and X. translucens, followed by thyme and rosemary against R. tritici and herring flower against X. translucens. Although the clinical use of ethanolic extracts and essential oils of the studied plants seems valuable due to side effects, but for the clinical use of essential oils and extracts, more research should be done on the mechanism of action of effective compounds of these plants on microbial agents.
Type of Study: Research |
Subject:
Herbal Drugs
Received: 2021/05/26 | Accepted: 2022/02/1 | Published: 2022/04/3
Received: 2021/05/26 | Accepted: 2022/02/1 | Published: 2022/04/3
References
1. Beigomi M, Biabangard A, Rohani R. Evaluation of antimicrobial effects of Rosemary and Withania somnifera methanol extract prepared by ultrasound waveform on Escherichia coli biofilm isolated from urinary tract infection. Micro Environer 2021;1(2):17-25.
2. Beigomi M, shakoory-moghadam V, Biabangard A, Behzadmehr R. Evaluation of the antimicrobial activity of plant extracts on Escherichia coli and Candida albicans. Micro Environer 2021;1(2):86-92.
3. Chagona P, Kwamboka N, Gaya H, Makonde H, Adem A, Osano K, et al. Phytochemical Analysis and Antibacterial Activity of the Kenyan Wild Orchids. Micro Environer 2021;1(2):93-100.
4. Ghafari M, Beigomi Z, Javadian E. Evaluation of antibacterial activity of extract plant against Staphylococcus aureus and Candida albicans isolated from women Micro Environer 2021;1(2):78-85.
5. Jahantigh M, ahmadi H. Analysis of the antimicrobial activity of Ashurak extracts prepared with different solvents on Klebsiella pneumoniae and Shigella dysentery isolated from poultry faeces. Micro Environer 2021;1(1):54-62.
6. Karabulut F, Aydın S, Parray JA. Interactions of antioxidant defense mechanisms developed by plants and microorganisms against pesticides Micro Environer 2021;1(2):63-77.
7. Karabulut F, Parray JA, Mir MY. Emerging trends for Harnessing plant metabolome and microbiome for sustainable food Production. Micro Environer 2021;1(1):33-53.
8. Parray JA, Ali U, Mir MY, Shameem N. A high throughputs and consistent method for the sampling and isolation of Endophytic bacteria allied to high altitude the medicinal plant Arnebia benthamii (Wall ex. G. Don). Micro Environer 2021;1(1):1-6.
9. Shafi S, Bandh SA, Shameem N. Interpreting Proteobacteria diversity through 16S rRNA analysis in Manasbal Lake, Kashmir. Micro Environer 2021;1(1):7-16.
10. Shahraki-Mojahed L, Behzadmehr R, Beigomi Z. Antimicrobial Effects of Ethanol, Methanol and Ethyl Acetate Teucrium polium and Citrullus colocynthis extract on Pseudomonas aeruginosa. Micro Environer 2021;1(1):26-32.
11. Fazeli-nasab B, Fooladvand Z. Classification and Evaluation of medicinal plant and medicinal properties of mastic. Int J Adv Biolog Biomed Res 2014;2(6):2155-61.
12. Mehrabi A-A, Fazeli-Nasab B. In vitro culture of Allium scorodoprasum spp. Rotundum: callus induction, somatic embryogenesis and direct bulblet formation. Intl J Agri Crop Sci 2012;4(1):1-7.
13. Fazeli-nasab B, Fooladvand Z. A Review on Iranian Carum copticum (L.): Composition and Biological Activities. Europ J Med Plants 2016;12(1):1-8.
14. Rezaei-Nasab M, Komeili G, Fazeli-Nasab B. Gastroprotective effects of aqueous and hydroalcholic extract of Scrophularia striata on ethanol-induced gastric ulcers in rats. Der Pharmacia Lettre 2017;9(5):84-93.
15. Valizadeh M, Beigomi M, Fazeli-Nasab B. Antibacterial and Anti biofilm effects of ethanol and aceton leaf extract of Momordica charantia and Tecomella undulata against Acinetobacter baumannii. Int J Adv Biol Biomed Res 2020;8(4):403-18.
16. Maghsoudi A, Saeidi S. Evaluation of Antimicrobial Activity of Ethanol Extract of Nine Zagros Native Medicinal Plants Against Salmonella Typhimurium. J Vet Res 2020;75(3):380-9.
17. Zgurskaya H, Evtushenko L, Akimov V, Kalakoutskii L. Rathayibacter gen. nov., including the species Rathayibacter rathayi comb. nov., Rathayibacter tritici comb. nov., Rathayibacter iranicus comb. nov., and six strains from annual grasses. Int J Systematic Evolutionary Microbiol 1993;43(1):143-9.
18. Riley IT, Ophel KM. Clavibacter toxicus sp. nov., the bacterium responsible for annual ryegrass toxicity in Australia. Int J Systematic Evolutionary Microbiol 1992;42(1):64-8.
19. Scharif G. Corynebacterium iranicum sp. nov. on Wheat (Triticum vulgare L.) in Iran, and a comparative study of it with C. tritici and C. rathayi. Entomologie et Phytopathologie Appliquées. 1961;19:1-24.
20. Babaeezad V, Rahimian H. Identity and distribution of Rathayibacter species causing gummy spike blight of wheat in some wheat growing areas of Iran. Iranian JPlant Pathol 2002;38(1-2):47-56.
21. Nik Akhtar S, Rahimian HA, Baba Zad VA. Evaluation of the phenotypic and genotypic diversity of the bacterial species causing gummy spike blight of wheat and barley. Agricul Biotechnol J 2016;8(1):139-56.
22. Brenner DJ, Krieg N, Staley J, Garrity G. Bergey’s manual of systematic bacteriology, Vol 2: The Proteobacteria. 2005. p. 1106 Pages.
23. Alimadadi N, Soudi MR, Sepehr S, Ghadam P. Potentially virulent isolates of Xanthomonas campestris from agricultural soil of Ray and Karaj. Cellular Molecular Res 2013;26(3):339-52.
24. Sapkota S, Mergoum M, Liu Z. The translucens group of Xanthomonas translucens: Complicated and important pathogens causing bacterial leaf streak on cereals. Molecular plant Pathol 2020;21(3):291-302.
25. Miguel MG, Cruz C, Faleiro L, Simões MT, Figueiredo AC, Barroso JG, et al. Foeniculum vulgare essential oils: chemical composition, antioxidant and antimicrobial activities. Natural Product Communications. 2010;5(2):319-28.
26. Soylu S, Soylu E, Evrendilek G. Chemical composition and antibacterial activity of essential oils of bitter fennel (Foeniculum vulgare Mill. var. vulgare) and dill (Anethum graveolens L.) against the growth of food-borne and seed-borne pathogenic bacteria. Italian J Food Sci 2009;21(3):347-55.
27. Vahidi H, Kamalinejad M, Sedaghati N. Antimicrobial properties of Croccus sativus L. Iran J Pharmac Res 2010;1(1):33-5.
28. Kulak M, Gul F, Sekeroglu N. Changes in growth parameter and essential oil composition of sage (Salvia officinalis L.) leaves in response to various salt stresses. Industrial Crops Prod 2020;145:112078.
29. Khodadadi S, Mahdinezhad N, Fazeli-Nasab B, Heidari MJ, Fakheri B, Miri A. Investigating the Possibility of Green Synthesis of Silver Nanoparticles Using Vaccinium arctostaphlyos Extract and Evaluating Its Antibacterial Properties. Biomed Res Int 2021;2021:Article ID: 5572252. doi:https://doi.org/10.1155/2021/5572252.
30. Aziman N, Abdullah N, Bujang A, Mohd Noor Z, Abdul Aziz A, Ahmad R. Phytochemicals of ethanolic extract and essential oil of Persicaria hydropiper and their potential as antibacterial agents for food packaging polylactic acid film. J Food Safety 2021;41(1):e12864.
31. Humphries RM, Ambler J, Mitchell SL, Castanheira M, Dingle T, Hindler JA, et al. CLSI methods development and standardization working group best practices for evaluation of antimicrobial susceptibility tests. J Clin Microbiol 2018;56(4):01934-17.
32. Pakdel F, Ghasemi S, Babaloo A, Javadzadeh Y, Momeni R, Ghanizadeh M, et al. Antibacterial effects of garlic extracts and ziziphora essential oil on bacteria associated with peri-implantitis. Journal of clinical and diagnostic research: JCDR 2017;11(4):ZC16.
33. Tabibnejad M, Arjomandzadegan M, Alikhani MY, Sadrnia M, Habibi G, Naseri Z. Comparison of the Effects of Hypericum perforatum extract and its Microemulsion with Aloe vera Extract on Brucella melitensis. Avicenna J Clin Med 2018;24(4):336-44.
34. Fazeli-Nasab B, Rahnama M, Shahriari S. The antimicrobial properties of hydro-alcoholic extracts of 29 medicinal plants on E. Coli and Staphylococcus aureus microbes. New Findings Vet Microbiol 2019;1(2):1-15.
35. Hamzekhani F, Mohammadi P, Saboora A. Antimicrobial effects of Leptogium saturninum, Ramalina peruviana and Punctelia borreri. Appl Biol 2020;33(2):33-45.
36. Aghajani Ghara M, Nematzadeh G, Kazemitabar Sk, Alavi sM. In vitro antibacterial activity of some plant essential oils against Pseudomonas syringae pv. Syringae and Rathayibacter tritici. J Med Plants Biotechnol 2020;5(2):42-56.
37. Reyes-Jurado F, Cervantes-Rincón T, Bach H, López-Malo A, Palou E. Antimicrobial activity of Mexican oregano (Lippia berlandieri), thyme (Thymus vulgaris), and mustard (Brassica nigra) essential oils in gaseous phase. Industrial Crops Prod 2019;131:90-5.
38. Behbahani BA, Shahidi F, Yazdi FT, Mortazavi SA, Mohebbi M. Antioxidant activity and antimicrobial effect of tarragon (Artemisia dracunculus) extract and chemical composition of its essential oil. JFood Measurement and Characterization. 2017;11(2):847-63.
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |