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Volume 6, Issue 2 (7-2024)                   pbp 2024, 6(2): 95-102 | Back to browse issues page

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Mami S, Veisi K, Azizian H, Mami S, Noorikia P, Mohamadi G et al . Investigating the Impact of a polyherbal Syrup Containing Licorice, Peppermint, and Chamomile Extracts on Inflammation and Peptic Ulcers in Male Rats. pbp 2024; 6 (2) :95-102
URL: http://pbp.medilam.ac.ir/article-1-255-en.html
Investigating the Impact of a polyherbal Syrup Containing Licorice, Peppermint, and Chamomile Extracts on Inflammation and Peptic Ulcers in Male Rats
Sajad Mami *1 , Kosar Veisi2 , Hajar Azizian3 , Sanaz Mami4 , Parisa Noorikia5 , Ghazal Mohamadi6 , Zohreh Rahmani5
1- Department of Laboratory and Clinical Sciences, Faculty of Veterinary Sciences, ilam university, Ilam, Iran , s.mami@ilam.ac.ir
2- Department of Microbiology, Ilam Branch, Islamic Azad University, Ilam, Iran
3- Phd of Comparative Histology, Ilam University, Ilam, Iran
4- Assistant Professor of Medical Immunology, Ilam University of Medical Science ,Ilam ,Iran
5- Department of Microbiology , Ilam Branch, Islamic Azad University, Ilam Iran
6- Department of Microbiology, Ilam Branch, Islamic Azad University, Ilam Iran
Abstract:   (1010 Views)

Objective: Peptic ulcers are caused by erosion of the gastric mucosa, often triggered by excessive gastric acid secretion, aspirin use, or Helicobacter pylori infection. These ulcers can lead to pain, inflammation, and bleeding. Although conventional therapies, including antibiotics and surgery, are effective, they are frequently associated with side effects and significant economic burden. Medicinal plants, valued for their anti-inflammatory and gastroprotective properties, are widely used in traditional medicine. This study aimed to evaluate the combined effect of hydroalcoholic extracts of licorice (Glycyrrhiza glabra), peppermint (Mentha piperita), and chamomile (Matricaria chamomilla) on peptic ulcer healing, compared with the effects of individual extracts.

Method: Licorice, peppermint, and chamomile plants were collected from the Zagros Mountains (Ilam Province, Iran), authenticated, and extracted using the Soxhlet method. Forty-nine male rats were divided into seven groups, with gastric ulcers induced by aspirin administration and Helicobacter exposure. Animals were anesthetized with ketamine and xylazine and euthanized according to ethical guidelines. Stomachs were excised for macroscopic and histopathological analysis of ulcer healing, inflammation, and epithelial regeneration.

Results: Chamomile and peppermint-treated groups showed slow and incomplete ulcer healing after 21 days, with persistent inflammation and bleeding in deeper tissues. Licorice-treated rats exhibited more pronounced recovery, with significant epithelial regeneration, although some ulcer depth remained unhealed. The polyherbal-treated group demonstrated marked improvement of gastric wall tissue, with advanced epitheliogenesis and near-complete healing comparable to the standard Recolic-treated group. Minimal inflammatory cells and vascular congestion persisted in some areas.

Conclusion: The combination of licorice, peppermint, and chamomile extracts promoted superior gastric ulcer healing compared to individual plant treatments, supporting the therapeutic potential of polyherbal formulations. Further experimental and clinical studies are warranted to optimize dosage, validate efficacy, and explore their potential integration into peptic ulcer management strategies.

Keywords: Digestive system, Stomach, Gastric ulcer, Medicinal plants, Treatment
Full-Text [PDF 1816 kb]   (186 Downloads)    
Type of Study: Research | Subject: Clinical
Received: 2024/03/20 | Accepted: 2024/09/14 | Published: 2024/09/21
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