Volume 7, Issue 4 (10-2025)
pbp 2025, 7(4): 140-149 |
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Patel V, S. Acharya N. In Vitro Anti-Urolithiatic Activity of Aqueous Extract of Macrotyloma uniflorum. pbp 2025; 7 (4) :140-149
URL: http://pbp.medilam.ac.ir/article-1-317-en.html
URL: http://pbp.medilam.ac.ir/article-1-317-en.html
1- Professor, Head Department of Pharmacology and Pharmacy Practice, Saraswati Institute of Pharmaceutical Sciences , vaibhavbpatel@yahoo.com
2- Institute of Pharmacy, Nirma University, Ahmedabad, Gujarat, India
2- Institute of Pharmacy, Nirma University, Ahmedabad, Gujarat, India
Abstract: (281 Views)
Objective: Urolithiasis is one of the oldest and most widespread urinary system diseases affecting millions globally. Herbal treatment is necessary to prevent and cure stone formation because of the high incidence and recurrence rate of stone formation, which is linked to significant discomfort and inflammation in the urinary system. Urolithiasis is treated with a variety of herbal preparations in India, which are renowned for their undeniable safety and effectiveness. In India, Macrotyloma uniflorum seeds are frequently used to treat kidney stones. The present study evaluates in vitro effect of aqueous extract of M. uniflorum (AEMU) on calcium oxalate crystallization.
Methods: Different concentrations of an AEMU (250–1500 µg/mL) were tested for in vitro activity. The outcomes were contrasted with those of a common herbal product that is sold on the market, Cystone. A fixed concentration of calcium chloride was incubated with different concentrations of sodium oxalate (2–10 mmol/mL) for nucleation and (2–3.5 mmol/mL) for crystal growth in order to perform the nucleation and crystal growth assay and assess the inhibitory effect of AEMU at various oxalate concentration levels. Assays for the aggregation and dissolution of calcium oxalate crystals were conducted and the results compared to the standard effects. Every step was carried out three times, and the analysis was done in Microsoft Excel to determine the percentage inhibition and dissolution in comparison to a standard control Cystone.
Results: When tested at various sodium oxalate concentrations, the results demonstrated that AEMU possesses exceptional calcium oxalate crystallization inhibitory activity in nucleation rate, crystal development, crystal aggregation and increased crystal dissolution. Compared to the herbal medication Cystone, a greater effect was seen in the dose-dependent manner in AEMU.
Conclusion: These in vitro results offer convincing proof of AEMU's potent crystallization inhibition and crystal dissolving capabilities.
Methods: Different concentrations of an AEMU (250–1500 µg/mL) were tested for in vitro activity. The outcomes were contrasted with those of a common herbal product that is sold on the market, Cystone. A fixed concentration of calcium chloride was incubated with different concentrations of sodium oxalate (2–10 mmol/mL) for nucleation and (2–3.5 mmol/mL) for crystal growth in order to perform the nucleation and crystal growth assay and assess the inhibitory effect of AEMU at various oxalate concentration levels. Assays for the aggregation and dissolution of calcium oxalate crystals were conducted and the results compared to the standard effects. Every step was carried out three times, and the analysis was done in Microsoft Excel to determine the percentage inhibition and dissolution in comparison to a standard control Cystone.
Results: When tested at various sodium oxalate concentrations, the results demonstrated that AEMU possesses exceptional calcium oxalate crystallization inhibitory activity in nucleation rate, crystal development, crystal aggregation and increased crystal dissolution. Compared to the herbal medication Cystone, a greater effect was seen in the dose-dependent manner in AEMU.
Conclusion: These in vitro results offer convincing proof of AEMU's potent crystallization inhibition and crystal dissolving capabilities.
Type of Study: Research |
Subject:
Herbal Drugs
Received: 2025/06/23 | Accepted: 2025/09/1 | Published: 2025/09/20
Received: 2025/06/23 | Accepted: 2025/09/1 | Published: 2025/09/20
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