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Patel V. In vitro anti-urolithiatic activity of aqueous extract of Macrotyloma uniflorum. pbp 2025; 7 (In press)
URL: http://pbp.medilam.ac.ir/article-1-317-en.html
URL: http://pbp.medilam.ac.ir/article-1-317-en.html
Professor, Head Department of Pharmacology and Pharmacy Practice, Saraswati Institute of Pharmaceutical Sciences , vaibhavbpatel@yahoo.com
Abstract: (9 Views)
Introduction: Urolithiasis is one of the oldest and most widespread urinary system diseases suffered globally. Due to high incidence and recurrence rate of stone formation associated with severe pain, inflammation in urinary system, which demand herbal treatment for preventing and cure the stone formation. In India, many herbal formulations are in use for the treatment of urolithiasis, known for its unquestionable efficacy and safety. Macrotyloma uniflorum seeds are widely used for renal stone activity in India.
Objective: The present study evaluates in vitro effect of aqueous extract of M. uniflorum (AEMU) on calcium oxalate crystallization.
Material & Methods: An AEMU at different concentration (250 - 1500 µg/ml) was subjected to in vitro activity. The results were compared with a standard herbal product (Cystone) available in market. The nucleation and crystal growth assay were performed taking different concentration of sodium oxalate (2-10 mmol/ml) for nucleation and (2-3.5 mmol/ml) for crystal growth and evaluate the inhibitory effect of AEMU at different concentration level of oxalate. Calcium oxalate crystal aggregation and dissolution assays were performed and compared with the effects observed in standard.
Results: The results showed that AEMU has excellent calcium oxalate crystallization inhibition activity in nucleation rate and crystal growth when evaluated at different concentration levels of oxalate. Inhibition of calcium oxalate crystal aggregation and crystal dissolution were observed in dose dependent manner.
Conclusion: AEMU showed the crystallization inhibition and crystal dissolution properties suggest strong scientific evidence for its use in renal stones.
Objective: The present study evaluates in vitro effect of aqueous extract of M. uniflorum (AEMU) on calcium oxalate crystallization.
Material & Methods: An AEMU at different concentration (250 - 1500 µg/ml) was subjected to in vitro activity. The results were compared with a standard herbal product (Cystone) available in market. The nucleation and crystal growth assay were performed taking different concentration of sodium oxalate (2-10 mmol/ml) for nucleation and (2-3.5 mmol/ml) for crystal growth and evaluate the inhibitory effect of AEMU at different concentration level of oxalate. Calcium oxalate crystal aggregation and dissolution assays were performed and compared with the effects observed in standard.
Results: The results showed that AEMU has excellent calcium oxalate crystallization inhibition activity in nucleation rate and crystal growth when evaluated at different concentration levels of oxalate. Inhibition of calcium oxalate crystal aggregation and crystal dissolution were observed in dose dependent manner.
Conclusion: AEMU showed the crystallization inhibition and crystal dissolution properties suggest strong scientific evidence for its use in renal stones.
Type of Study: Research |
Subject:
Herbal Drugs
Received: 2025/06/23 | Accepted: 2025/07/29 | Published: 2025/12/1
Received: 2025/06/23 | Accepted: 2025/07/29 | Published: 2025/12/1
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