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Sepordeh Seydani S, Shams Moattar F. Effect of Zinc Oxide Nanoparticles and Calcium Ions on Thermal Stability of Peroxidase in Calamintha officinalis Moench. pbp 2026; 8
URL: http://pbp.medilam.ac.ir/article-1-346-en.html
URL: http://pbp.medilam.ac.ir/article-1-346-en.html
1- Department of Microbiology, Faculty of Basic Sciences, Lahijan Branch, Islamic Azad University, Lahijan, Iran.
2- Department of Microbiology, Faculty of Basic Sciences, Lahijan Branch, Islamic Azad University, Lahijan, Iran. ,fshams@ymail.com
2- Department of Microbiology, Faculty of Basic Sciences, Lahijan Branch, Islamic Azad University, Lahijan, Iran. ,
Abstract: (128 Views)
Objective: The antioxidant activity of plant extracts and essential oils is particularly valuable due to their beneficial physiological effects and potential as natural alternatives to synthetic antioxidants. A major limitation in industrial applications of plant biomolecules is their low thermal stability. This study investigated the effects of zinc oxide nanoparticles (ZnO NPs) and calcium ions on enhancing the thermal stability of peroxidase (POD) from Calamintha officinalis Moench (COM).
Methods: Peroxidase activity was first assessed to determine the enzyme’s optimal pH and temperature. The impact of ZnO NPs on the thermal stability of POD was evaluated at various temperatures and nanoparticle concentrations ranging from 0.01 to 0.00001 mg/mL. Similarly, the effect of calcium ions on enzyme stability was tested at identical concentrations. A control group without Ca²⁺ or ZnO NPs was included for comparison.
Results: The optimal pH and temperature of COM peroxidase were 6.8 and 25 °C, respectively, with maximum thermal stability observed at 40 °C. ZnO NPs at different concentrations did not significantly affect enzyme activity. In contrast, low concentrations of calcium ions (0.00001 mg/mL) enhanced enzyme activity during the first hour of incubation.
Conclusion: Zinc oxide nanoparticles did not alter the activity of COM peroxidase under the conditions tested. However, calcium ions appear to positively influence the thermal stability of the enzyme, suggesting a potential role in enhancing the functional performance of plant-derived peroxidases
Methods: Peroxidase activity was first assessed to determine the enzyme’s optimal pH and temperature. The impact of ZnO NPs on the thermal stability of POD was evaluated at various temperatures and nanoparticle concentrations ranging from 0.01 to 0.00001 mg/mL. Similarly, the effect of calcium ions on enzyme stability was tested at identical concentrations. A control group without Ca²⁺ or ZnO NPs was included for comparison.
Results: The optimal pH and temperature of COM peroxidase were 6.8 and 25 °C, respectively, with maximum thermal stability observed at 40 °C. ZnO NPs at different concentrations did not significantly affect enzyme activity. In contrast, low concentrations of calcium ions (0.00001 mg/mL) enhanced enzyme activity during the first hour of incubation.
Conclusion: Zinc oxide nanoparticles did not alter the activity of COM peroxidase under the conditions tested. However, calcium ions appear to positively influence the thermal stability of the enzyme, suggesting a potential role in enhancing the functional performance of plant-derived peroxidases
Keywords: Peroxidase, Thermal Stability, Calamintha officinalis, Zinc Oxide Nanoparticles, Calcium Ions
Type of Study: Research |
Subject:
Herbal Drugs
Received: 2025/08/30 | Accepted: 2025/09/13 | Published: 2025/12/1
Received: 2025/08/30 | Accepted: 2025/09/13 | Published: 2025/12/1
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