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Sepordeh Seydani S, Shams Moattar F. Effect of Zinc Oxide Nanoparticles and Calcium Ions on Thermal Stability of Peroxidase in Calamintha officinalis Moench. pbp 2025; 7 (In press)
URL: http://pbp.medilam.ac.ir/article-1-346-en.html
URL: http://pbp.medilam.ac.ir/article-1-346-en.html
1- Department of Microbiology, Faculty of Basic Sciences, Lahijan Branch, Islamic Azad University, Lahijan, Iran.
2- Department of Microbiology, Faculty of Basic Sciences, Lahijan Branch, Islamic Azad University, Lahijan, Iran. ,fshams@ymail.com
2- Department of Microbiology, Faculty of Basic Sciences, Lahijan Branch, Islamic Azad University, Lahijan, Iran. ,
Abstract: (25 Views)
Objective: The antioxidant activity of plant extracts and essential oils is particularly important because of their beneficial physiological effects on cells and their potential for substitution by artificial antioxidants. One of the limitations in the use of active plant biomolecules in industry is their low thermal stability. To investigate the effect of zinc oxide nanoparticles (ZnO NPs) and calcium ions on enhancing the thermal stability of peroxidase enzyme (POD) from Calamintha officinalis Moench (COM), the present study was conducted.
Materials and Methods: In this study, after assessing the activity of peroxidase, the optimal pH and temperature of peroxidase were measured. The effect of nanoparticles on the thermal stability of the peroxidase enzyme was investigated at different temperatures and concentrations of 0.01 to 0.00001 mg/mL. Finally, the effect of calcium ions on the thermal stability of the desired enzyme was also measured at concentrations of 0.01 to 0.00001mg/mL. The control group was also considered to be the solution without Ca²⁺ and ZnO NP.
Results: The optimal pH and temperature of the peroxidase enzyme were reported as 6.8 and 25 °C, respectively, with the highest thermal stability at 40 °C. There was no significant difference between the activity of the enzyme in different concentrations of zinc oxide nanoparticles. Based on our results, the activity of the desired enzyme in the presence of different concentrations of calcium showed that lower concentrations (0.00001mg/mL) led to an increase in enzyme activity in the first 1 hour.
Conclusion: According to our results, enzyme activity did not change at different concentrations of zinc oxide nanoparticles, but calcium ions may affect the thermal stability of peroxidase in the
COM plant.
Materials and Methods: In this study, after assessing the activity of peroxidase, the optimal pH and temperature of peroxidase were measured. The effect of nanoparticles on the thermal stability of the peroxidase enzyme was investigated at different temperatures and concentrations of 0.01 to 0.00001 mg/mL. Finally, the effect of calcium ions on the thermal stability of the desired enzyme was also measured at concentrations of 0.01 to 0.00001mg/mL. The control group was also considered to be the solution without Ca²⁺ and ZnO NP.
Results: The optimal pH and temperature of the peroxidase enzyme were reported as 6.8 and 25 °C, respectively, with the highest thermal stability at 40 °C. There was no significant difference between the activity of the enzyme in different concentrations of zinc oxide nanoparticles. Based on our results, the activity of the desired enzyme in the presence of different concentrations of calcium showed that lower concentrations (0.00001mg/mL) led to an increase in enzyme activity in the first 1 hour.
Conclusion: According to our results, enzyme activity did not change at different concentrations of zinc oxide nanoparticles, but calcium ions may affect the thermal stability of peroxidase in the
COM plant.
Keywords: Peroxidase, Thermal Stability, Calamintha officinalis, Zinc Oxide Nanoparticles, Calcium Ions
Type of Study: Research |
Subject:
Herbal Drugs
Received: 2025/08/30 | Accepted: 2025/09/13 | Published: 2025/12/1
Received: 2025/08/30 | Accepted: 2025/09/13 | Published: 2025/12/1
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