Volume 4, Issue 2 (12-2022)                   PBP 2022, 4(2): 64-75 | Back to browse issues page

XML Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Usunobun U, Imoru O. Evaluation of Hepatoprotective Potential of Chromolaena odorata (L.) R.M. King & H.Rob. Against Methotrexate-induced Hepatic Toxicity in Rats. PBP 2022; 4 (2) :64-75
URL: http://pbp.medilam.ac.ir/article-1-126-en.html
Edo State University Uzairue, Nigeria , usunobun.usunomena@edouniversity.edu.ng
Abstract:   (545 Views)
Objective: Liver, as an organ, is predominantly responsible for the metabolism of drugs, alcohol, and foreign chemicals; hence, it is vulnerable to injury that may result in different liver diseases. The protective effect of Chromolaena odorata (L.) R.M. King & H. Rob. aqueous leaf extract against Methotrexate-induced hepatotoxicity in male wistar rats was investigated.
Material and Methods: 24 male rats were divided into four groups of 6. Group one received normal saline only and served as control while group two received 200mg/kg Ch. odorata aqueous extract daily for ten consecutive days. Group three received intraperitoneally, a single dose of 20mg/kg Methotrexate on the ninth day of treatment while group four received 200mg/kg Ch. Odorata aqueous extract for ten consecutive days and a single dose of 20mg/kg Methotrexate intraperitoneally on ninth day of the study.
Results: Results showed that Methotrexate-induced hepatotoxicity decreased liver synthetic molecules (Total Protein and Albumin); decreased endogenous antioxidants (Catalase and Superoxide dismutase); increased liver function enzymes (Alanine aminotransferase and Aspartate aminotransferase), and increased lipid peroxidation (Malondialdehyde). Corroborating biochemical assessment, Histopathological analysis of untreated Methotrexate rats showed liver sections with focal necrosis, dilation and congestion of central vein and portal vein whereas aqueous leaf extract of Ch. odorata reduced the degree of lesions with seen improvement and reduction of hepatocytes degeneration.
Conclusion: Thus, Ch. odorata (L.) offered protection to the liver from damage caused by Methotrexate attributable to its active bioactive agents including flavonoids which scavenges free radicals, enhanced the antioxidant status and protected against oxidative damage and oxidative stress.
Full-Text [PDF 2996 kb]   (55 Downloads)    
Type of Study: Research | Subject: Phyto-pharmacology
Received: 2022/06/8 | Accepted: 2022/06/11 | Published: 2022/06/15

1. Friedman PF. GI/Liver Secrets: with student consult Access. Saint Louis: C.V. Mosby. 2006
2. Bayram M, Ozogul C, Dursun A, Ercan ZS, Isik, I, Dilekoz E. Ligth and electron microscope examination of the effects of methotrexate on the endosalpinx. European J Obstet & Gyneco Repro Bio 2005; 120: 96–103. doi: 10.1016/j.ejogrb.2004.08.014.
3. Sener G, Demiralp EE, Cetiner, M, Ercan FS¸ irvanc S, Gedik N, Yegen, BC. L-carnitine ameliorates methotrexate-induced oxidative organ injury and inhibits leukocyte death. Cell Biol Toxicol 2006; 22: 47–60. doi: 10.1007/s10565-006-0025-0.
4. Uraz S, Tahan V, Aygun C, Eren F, Unluguzel G, Yuksel M, Senturk O, Avsar E, Haklar G, Celikel C, Hulagu S, Tozun N. Role of ursodeoxycholic acid in prevention of methotrexate-induced liver toxicity. Dig Dis Sci 2008; 53, 1071–7. https://doi.org/10.1007/s10620-007-9949-3
5. Tanaka H, Sato M, Fujiwara S. Antibacterial Activity of Isoflavonoids Isolated from Erythrinavariegata Against Methicil-lin-resistant Staphylococcus aureus. Letters in Appl Micro 2002; 35: 494 – 498. doi: 10.1046/j.1472-765x.2002.01222.x.
6. Ngozi N, Theresa O. Personal communication on the relevance and indigenous use of medicinal plants, 2014.
7. Odugbemi, T. Outlines and pictures of medicinal plants from Nigeria. University of Lagos Press, Lagos, Nigeria, 2006; Pp. 1-283.
8. Akinmoladun AC, Akinloye O. Effect of Cromolaena odorata on hypercholesterolemia related metabolic imbalances. Proc. Akure- Humbold Kellog/3rd SAAT Annual Conference, FUTA, Nigeria, 2007; pp 287-290.
9. Usunobun U, Ewere, GE. Phytochemical analysis, Mineral Composition and in vitro antioxidant activities of Chromolaena odorata leaves. ARC J Pharma Sci 2016; 2: 6-10. DOI: http://dx.doi.org/10.20431/2455-1538.0202003
10. Aladaileh SH, Omnia EH, Mohammad, HA, Saghir SAM, Bin-Jumah M, Manal AA, Mousa OG, Almaiman AA, Mahmoud AM. Formononetin Upregulates Nrf2/HO-1 Signaling and Prevents Oxidative Stress, Inflammation, and Kidney Injury in Methotrexate-Induced Rats. Antioxidants 2019; 8: 430. doi:10.3390/antiox8100430
11. Ijioma S, Okafor A, Ndukuba P, Nwankwo A, Akomas S. Hypogly‌cemic, hematologic and lipid profile effects of Chromolaena odorata ethanol leaf extract in alloxan induced diabetic rats. Annals of Bio‌l Sci 2014; 2: 27–32.
12. APA. Guidelines for Ethical Conduct in the Care and Use of Non-Human Animals in Research. American Psychological Association (APA), Washington DC, USA. Retrieved June 13, 2015 from https://www.apa.org/science/leadership/care/careanimal-
13. Reitman S, Frankel S. A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. Am J Clin Pathol 1957; 28: 56–63. https://doi.org/10.1093/ajcp/28.1.56
14. Jendrassik L, Grof P. Total and Direct Bilirubin. Biochem 1938; 7297: 81.
15. Tietz NW, Burtis CA, Ashwood ER. Tietz textbook of clinical chemistry. Saunders, 1994.
16. Doumas BT, Watson WA, Biggs HG. Albumin standards and the measurement of serum albumin with bromcresol green. Clin Chim Acts. 1971; 31: 87–96. https://doi.org/10.1016/0009-8981(71)90365-2.
17. Ohkawa H, Ohishi N, Yogi K. Assay for lipid peroxidation in animal tissues by thiobarbituric acid reaction. Ann Biochem 1979; 95: 351–358. Doi: 10.1016/0003-2697(79)90738-3
18. Misra HP, Fridovich I. The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. J Biol Chem 1972; 247: 3170–3175.
19. Cohen G, Dembiee D, Marcus J. Measuremment of catalase activity in tissues extracts. Anal Biochem 1970; 34, 30-38. Doi: 10.1016/0003-2697(70)90083-7
20. Gregory SH, Wing, EJ. Neutrophil−Kupffer cell interaction in host defenses to systemic infections. Immunol To 1998; 19: 507−510. doi: 10.1016/s0167-5699(98)01319-x
21. Lee HJ, Seo HS, Kim GJ, Jeon CY, Park JH, Jang BH, Park SJ, Shin, YC, Ko SG. Houttuynia cordata thumb inhibits the production of pro-inflammatory cytokines through inhibition of the NF-κB signaling pathway in HMC-1 human mast cells. Mol Med Rep 2013; 8: 731−736. doi: 10.3892/mmr.2013.1585
22. Abbate GM, Sacerdote P, Amodeo G, Mangano A, Levrini L. Experimentally induced pulpal lesion and substance P expression: effect of ketoprofen A preliminary study. Int J Dent 2016; 1−5. https://doi.org/10.1155/2016/6820781
23. Ito F, Sono Y, Ito T. Measurement and clinical significance of lipid peroxidation as a biomarker of oxidative stress: oxidative stress in diabetes, atherosclerosis, and chronic inflammation, Antioxidants. 2019; 8: 72. https://doi.org/10.3390/ antiox8030072
24. Usunobun U, Ugbeni CO, Agu KC. Protective effect of Celosia argentea aqueous leaf extract on liver function in Carbon-tetrachloride (CCl4)-induced hepatotoxicity. Malaysian J Biochem Mol Bio 2019; 22; 93 – 96.
25. Hany E, Abdulmohsen IA, Manal A, Mahmoud K, Omar ME, Azza S, Ashraf MA. Naringin alleviates methotrexate-induced liver injury in male albino rats and enhances its antitumor efficacy in HepG2 cells. Bioscience Reports 2020; 40, BSR20193686. https://doi.org/10.1042/BSR20193686
26. Moghadam, AR, Tutunchi, S, Ali Namvaran-Abbas-Abad Yazdi M, Bonyadi F, Daryoush M, Mohammad M, Marzban H, Marek JL, Ghavami S. Pre-administration of turmeric prevents methotrexate-induced liver toxicity and oxidative stress. BMC Comp Altern Med 2015; 15: 246. https://doi 10.1186/s12906-015-0773-6
27. Adikwu E, Bokolo B, Odeghe OB. Ethanolic Leaf Extract of Ocimum gratissimum Abrogates Methotrexate-induced Liver Injury in Albino Rats. Asian J Bio Sci 2020; 13: 201-209. https://doi: 10.3923/ajbs.2020.201.209
28. Abo-Haded HM, Elkablawy MA, Al-johani, Z, Al-ahmadi O, El-Agamy DS. Hepatoprotective effect of sitagliptinagainst methotrexate induced liver toxicity. PLoS ONE 12, 2017; e0174295. https://doi.org/10.1371/journal.pone.0174295
29. Kumar M, Sharma VL, Sehgal A, Jain M. Protective effects of green and white tea against benzo(a)pyrene induced oxidative stress and DNA damage in murine model. Nutri Canc 2012; 64: 300–306. https://doi.org/10.1080/01635581.2012.648300
30. Uttara B, Singh AV, Zamboni P, Mahajan RT. “Oxidative stress and neurodegenerative diseases: a review of upstream and downstream antioxidant therapeutic options. Current Neuropharma 2009; 7: 65–74. doi: 10.2174/157015909787602823
31. Usunobun U, Ekakitie C, 2020. Restoration potential of Celosia argentea leaf on Acetaminophen-induced liver toxicity. An Res Int 2020; 17: 3674–3681.
31. Cure, E, Kirbas A, Tumkaya L, Cure MC, Kalkan Y, Yilmaz A, et al. Protective effect of infliximab on methotrexate-induced liver injury in rats: Unexpected drug interaction. J Can Res Ther 2015; 11: 164-169. Doi: 10.4103/0973-1482.140809
32. Osman AGM, Kloas W. Water Quality and Heavy Metal Monitoring in Water,
33. Sediments, and Tissues of the African Catfish Clarias gariepinus (Burchell, 1822) from the River Nile, Egypt. J Env Prot 2010; 1: 389-400. doi:10.4236/jep.2010.14045
34. Woreta TA, Alqahtani, SA. Evaluation of abnormal liver tests. Medical Clinics of North America, 2014; 98: 1–16. https://doi.org/10.1016/j.mcna.2013.09.005
35. Usunobun U, Osaigbovo JO, Okolie NP. Hepatoprotective effect of Rhaphiostylis beninensis Ethanol root extract on Carbon-tetrachloride (CCl4)-induced liver attack and damage in Rats. Amer J Biome Sci 2020; 12: 155-163. https://doi:10.5099/aj200300155
36. Mojtaba K, Heibatullah K, Mehdi G, Layasadat K, Samira B, Hadi K. Crocin ameliorates methotrexate-induced liver injury via inhibition of oxidative stress and inflammation in rats. Pharmac Reports 2019; 71: 746–752. http://dx.doi.org/10.1016/j.pharep.2019.04.004
37. Hemeida RA, Mohafez OM. Curcumin attenuates methotrexate-induced hepatic oxidative damage in rats. J Egyptian Nat Can Inst. 2008; 20: 141-148. PMID: 20029470

Add your comments about this article : Your username or Email:

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.