Volume 4, Issue 2 (12-2022)
pbp 2022, 4(2): 48-56 |
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Hassan A, O. A, M T, A. S M, A. J. Y. Phytochemical, Toxicological and In vitro Antifungal Studies of Mitracarpus hirtus L.. pbp 2022; 4 (2) :48-56
URL: http://pbp.medilam.ac.ir/article-1-142-en.html
URL: http://pbp.medilam.ac.ir/article-1-142-en.html
1- Department of Chemistry, Sokoto State University, Sokoto, Nigeria , hassan.abubakar@ssu.edu.ng
2- 2Department of Pharmaceutical Chemistry, Jamia Hamdard, New Delhi, India
3- Department of Chemistry, Sokoto State University, Sokoto, Nigeria
4- Department of Pharmaceutical and Medicinal Chemistry, Usmanu Danfodiyo University, Sokoto, Nigeria
2- 2Department of Pharmaceutical Chemistry, Jamia Hamdard, New Delhi, India
3- Department of Chemistry, Sokoto State University, Sokoto, Nigeria
4- Department of Pharmaceutical and Medicinal Chemistry, Usmanu Danfodiyo University, Sokoto, Nigeria
Abstract: (1984 Views)
Objective: Thus, in this work, phytochemical screening, acute toxicity and antifungal evaluation on the methanol extracts of different parts (leaves, seed and aerial part) of Mitracarpus hirtus against Candida albicans, Candida tropicalis, Aspergillus fumigatus, Aspergillus niger, Histoplasm capsulatum, Mucor sp, Microsporum canis, Microsporum gallinae, Trichophyton mentagrophyte, and Trichophyton rubrum using agar well diffusion and broth dilution techniques.
Material and Methods: The phytochemical screening of the methanol extracts of different parts of the plant revealed the presence of tannins saponins, flavonoids, alkaloids, steroids and terpenoids and the median lethal dose (LD50) was estimated to be greater than 5000 mg/kg which indicates that the plant is non-toxic.
Results: Susceptibility test for the methanol extracts showed mean zone of inhibition ranging from 19.33±0.58-23.33±0.56 mm against the test organisms while the standard drug, Fulcin had 24.33±0.58 – 31.67±0.58 mm. The minimum inhibitory concentration (MIC) of the extracts ranges between 0.25-0.50 mg/mL while the minimum fungicidal concentration (MFC) ranges between 0.25-1.00 mg/mL against some of the fungal pathogens.
Conclusion: The findings of this study have revealed the antifungal efficacy of methanol extracts of the different part of M. hirtus which supports the ethno-medicinal claim of the use of the plant in the treatment of fungal infections.
Material and Methods: The phytochemical screening of the methanol extracts of different parts of the plant revealed the presence of tannins saponins, flavonoids, alkaloids, steroids and terpenoids and the median lethal dose (LD50) was estimated to be greater than 5000 mg/kg which indicates that the plant is non-toxic.
Results: Susceptibility test for the methanol extracts showed mean zone of inhibition ranging from 19.33±0.58-23.33±0.56 mm against the test organisms while the standard drug, Fulcin had 24.33±0.58 – 31.67±0.58 mm. The minimum inhibitory concentration (MIC) of the extracts ranges between 0.25-0.50 mg/mL while the minimum fungicidal concentration (MFC) ranges between 0.25-1.00 mg/mL against some of the fungal pathogens.
Conclusion: The findings of this study have revealed the antifungal efficacy of methanol extracts of the different part of M. hirtus which supports the ethno-medicinal claim of the use of the plant in the treatment of fungal infections.
Type of Study: Research |
Subject:
Herbal Drugs
Received: 2022/07/17 | Accepted: 2022/12/1 | Published: 2022/12/1
Received: 2022/07/17 | Accepted: 2022/12/1 | Published: 2022/12/1
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