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Aliasghari Veshareh A, Amini R, Rabbani khorasgani M, Dehghan P, Roghanian R. Evaluation of the Antifungal Efficacy of Lactobacillus Strains Isolated from Human and Camel UrineAgainst Fluconazole-Resistant Candida spp.. pbp 2025; 7 (In press)
URL: http://pbp.medilam.ac.ir/article-1-306-en.html
URL: http://pbp.medilam.ac.ir/article-1-306-en.html
Azam Aliasghari Veshareh1 
, Reihaneh Amini2 , Mohammad Rabbani khorasgani *3 , Parvin Dehghan4 , Rasoul Roghanian2
, Reihaneh Amini2 , Mohammad Rabbani khorasgani *3 , Parvin Dehghan4 , Rasoul Roghanian2
1- Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran
2- Department of Cell and Molecular Biology & Microbiology, Faculty of Biological Science & Technology, University of Isfahan, Isfahan, Islamic Republic of IRAN
3- Department of Cell and Molecular Biology & Microbiology, Faculty of Biological Science & Technology, University of Isfahan, Isfahan, Islamic Republic of IRAN ,rabbani@ut.ac.ir
4- Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
2- Department of Cell and Molecular Biology & Microbiology, Faculty of Biological Science & Technology, University of Isfahan, Isfahan, Islamic Republic of IRAN
3- Department of Cell and Molecular Biology & Microbiology, Faculty of Biological Science & Technology, University of Isfahan, Isfahan, Islamic Republic of IRAN ,
4- Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
Abstract: (69 Views)
Background: The increasing prevalence of Candida infections and the emergence of resistance to chemical antifungal medicines, such as fluconazole, has prompted increased research into natural compounds with anti-Candida activity. Probiotics, in particular, are being investigated as promising alternatives due to their lower costs and reduced side effects compared to synthetic drugs.
Objective: This study aimed to isolate Lactobacillus strains from human and camel urine and evaluate their antifungal activity against three Candida strains isolated from human vaginal samples.
Materials and Methods: In this research, Lactobacillus strains were isolated from human and camel urine. Then, the anti-Candida effect of isolated Lactobacillus spp. and two commercial probiotic formulations, Lactofem and Femi capsules containing Lactobacillus spp. 109 and 1.65 × 109 CFU/mL, respectively, was assessed using the microtiter plate method for determination of minimum inhibitory concentration (MIC). Finally, the interaction of Lactobacillus and fluconazole against Candida was investigated using the checkerboard method.
Results: Lactobacillus brevis and Lactobacillus crispatus were isolated from human urine samples, and Lactobacillus brevis was isolated from camel urine. The MIC of Lactobacillus spp. against Candida albicans, Candida glabrata, and Candida krusei was 1.87 × 107 CFU/mL. Antifungal effects of Lactofem and Femi against three Candida spp. were 6.25 × 106 CFU/mL and 2.062 × 107 CFU/mL, respectively. Among the probiotics, Lactofem had the highest, and Femi had the lowest anti-Candida effect. Fractional Inhibitory Concentration results show the synergistic effect of the combination of 9.37 × 106 CFU/mL Lactobacillus and 4 μg/mL fluconazole.
Conclusions: The finding of this study indicate that Lactobacillus spp. have potent antifungal activity against Candida infections and show synergistic effect when combined with fluconazole, suggesting their potential as an alternative or complementary therapeutic approach in antifungal therapy. Future research is recommended to focus on developing clinical formulations and validating efficacy through in vivo studies.
may be recommended for their applications as an alternative or complementary therapeutic approach in pharmaceutical formulations.
Objective: This study aimed to isolate Lactobacillus strains from human and camel urine and evaluate their antifungal activity against three Candida strains isolated from human vaginal samples.
Materials and Methods: In this research, Lactobacillus strains were isolated from human and camel urine. Then, the anti-Candida effect of isolated Lactobacillus spp. and two commercial probiotic formulations, Lactofem and Femi capsules containing Lactobacillus spp. 109 and 1.65 × 109 CFU/mL, respectively, was assessed using the microtiter plate method for determination of minimum inhibitory concentration (MIC). Finally, the interaction of Lactobacillus and fluconazole against Candida was investigated using the checkerboard method.
Results: Lactobacillus brevis and Lactobacillus crispatus were isolated from human urine samples, and Lactobacillus brevis was isolated from camel urine. The MIC of Lactobacillus spp. against Candida albicans, Candida glabrata, and Candida krusei was 1.87 × 107 CFU/mL. Antifungal effects of Lactofem and Femi against three Candida spp. were 6.25 × 106 CFU/mL and 2.062 × 107 CFU/mL, respectively. Among the probiotics, Lactofem had the highest, and Femi had the lowest anti-Candida effect. Fractional Inhibitory Concentration results show the synergistic effect of the combination of 9.37 × 106 CFU/mL Lactobacillus and 4 μg/mL fluconazole.
Conclusions: The finding of this study indicate that Lactobacillus spp. have potent antifungal activity against Candida infections and show synergistic effect when combined with fluconazole, suggesting their potential as an alternative or complementary therapeutic approach in antifungal therapy. Future research is recommended to focus on developing clinical formulations and validating efficacy through in vivo studies.
may be recommended for their applications as an alternative or complementary therapeutic approach in pharmaceutical formulations.
Type of Study: Research |
Subject:
Clinical
Received: 2025/05/24 | Accepted: 2025/06/23 | Published: 2025/12/1
Received: 2025/05/24 | Accepted: 2025/06/23 | Published: 2025/12/1
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