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Shafaei M, ebrahimi M, Mokhtari A. Effect of Drying Methods on the Quantity of the Hypericin in the Adventitious Root of the St John's Wort (Hypericum perforatum L.). pbp 2026; 8 (In press)
URL: http://pbp.medilam.ac.ir/article-1-348-en.html
URL: http://pbp.medilam.ac.ir/article-1-348-en.html
1- Agricultural Biotechnology Research Institute of Iran, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran , mo.shafaee@gmail.com
2- Agricultural Biotechnology Research Institute of Iran, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran
2- Agricultural Biotechnology Research Institute of Iran, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran
Abstract: (28 Views)
Objective: Hypericum perforatum L. is a valuable medicinal plant known for its bioactive compound hypericin, which exhibits antidepressant and antiviral properties. This study explores the influence of various drying methods on hypericin content in adventitious roots, a key source of this compound.
Methods: Fresh adventitious roots with approximately 90% (w/w) moisture were subjected to three drying methods: shade-drying, oven-drying, and freeze-drying. The final moisture contents were 37%, 8%, and 5.3% (w/w), respectively. Hypericin content was quantified using high-performance liquid chromatography (HPLC). Hierarchical cluster analysis (HCA) was applied to assess the effects of drying methods across four adventitious root lines, with results visualized through dendrograms, boxplots, and bar charts.
Results: Freeze-drying preserved the highest hypericin levels, reaching up to 0.166 mg/g dry weight (DW), followed by oven-drying and shade-drying. Line 4 consistently exhibited superior hypericin content across all drying methods, indicating potential genetic or physiological advantages.
Discussion: The results demonstrate that freeze-drying is the optimal method for maximizing hypericin yield in adventitious roots of Hypericum perforatum L. The superior performance of Line 4 suggests that targeted selection of root lines could further enhance bioactive compound production. These findings provide valuable insights for optimizing drying techniques and root line selection in the production of hypericin-rich materials.
Methods: Fresh adventitious roots with approximately 90% (w/w) moisture were subjected to three drying methods: shade-drying, oven-drying, and freeze-drying. The final moisture contents were 37%, 8%, and 5.3% (w/w), respectively. Hypericin content was quantified using high-performance liquid chromatography (HPLC). Hierarchical cluster analysis (HCA) was applied to assess the effects of drying methods across four adventitious root lines, with results visualized through dendrograms, boxplots, and bar charts.
Results: Freeze-drying preserved the highest hypericin levels, reaching up to 0.166 mg/g dry weight (DW), followed by oven-drying and shade-drying. Line 4 consistently exhibited superior hypericin content across all drying methods, indicating potential genetic or physiological advantages.
Discussion: The results demonstrate that freeze-drying is the optimal method for maximizing hypericin yield in adventitious roots of Hypericum perforatum L. The superior performance of Line 4 suggests that targeted selection of root lines could further enhance bioactive compound production. These findings provide valuable insights for optimizing drying techniques and root line selection in the production of hypericin-rich materials.
Keywords: Hypericum perforatum, hypericin, adventitious roots, drying methods, freeze-drying, hierarchical cluster analysis
Type of Study: Research |
Subject:
Herbal Drugs
Received: 2025/07/5 | Accepted: 2025/09/21 | Published: 2026/01/20
Received: 2025/07/5 | Accepted: 2025/09/21 | Published: 2026/01/20
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