Showing 4 results for Nweje-Anyalowu
Dr Robert Ikechukwu Urokoir.uroko@mouau.edu.ng, Dr Agbafor Amarachi, Dr Paul Chukwuemaka Nweje-Anyalowu, Dr Ogwo Elisha Uko, Dr Precious Tobechi Abuachi,
Volume 3, Issue 2 (12-2021)
Abstract
The study assessed the hepatoprotective and therapeutic effects of methanol extract of Asystasia gangetica leaves (MEAGL) on rats induced liver injury with monosodium glutamate (MSG). In this study, fifty-four rats were selected into nine groups, with each of the groups having six rats. Group 1 was the negative control; group 2 was a positive control treated only with MSG, and group 3 was group treated with MSG + 100 mg/kg/day of silymarin. Groups 4 and 5 were extract groups administered with 200 and 500 mg/kg MEAGL/day, whereas groups 6 and 7 were hepatoprotectant groups pre-treated with 200 and 500 mg/kg MEAGL/day before MSG administration. Groups 8 and 9 were the therapeutic groups first treated with MSG and then orally administered with 200 and 500 mg/kg MEAGL/day, respectively. The positive group treated with MSG only had significantly (P< 0.05) elevated alanine transaminase (ALT), aspartate transaminase (AST) and alkaline phosphatase (ALP) activities relative to the negative control. MSG induction further caused a significant (P< 0.05) reduction in total protein and albumin concentrations and elevated total and direct bilirubin concentrations of the positive control relative to the negative control. In the groups of MEAGL pre-treated rats as well as the rats treated with MEAGL after being treated with MSG, significantly (P < 0.05) reduced ALT, AST and ALP activities and elevated total protein and albumin concentrations and improved liver architecture compared with the positive group treated only with MSG, were observed. The findings revealed that the MEAGL possess both hepatoprotective and therapeutic activities that could ameliorate MSG-induced hepatic disorders.
Robert Ikechukwu Uroko, Paul Chukwuemaka Nweje-Anyalowu, Chinomso Friday Aaron, Charles Nnanna Chukwu,
Volume 4, Issue 1 (6-2022)
Abstract
Objective: The combined extract of Spermacoce radiata and Hypselodelphys poggeana leaves has been shown to be a potent therapeutic agent against benign prostatic hyperplasia and it is used extensively for BPH and other diseases by local traditional medicine practitioners without any evaluation of its toxicity effects on liver and other vital organs in the body. The study evaluated the hepatoprotective effects of a combined extract of S. radiata and H. poggeana (CEESH) on rats with prostate enlargement (BPH).
Material and Methods: The study had five groups of rats (n = 6), with groups 1 and 2 being the normal and BPH controls, respectively. Groups 3, 4 and 5 were the BPH rats administered Finasteride, 300 and 600 mg/kg CEESH, respectively. Prostate enlargement was induced in the rat by the subcutaneous administration of testosterone injection (5 mg/kg/day for 28 days), while Finasteride and CEESH were respectively administered to the rats orally as therapeutic agents.
Results: Prostate enlargement in the rats elevated AST, ALT and ALP activities in BPH control coupled with a significant rise in the serum bilirubin levels compared to the normal control. The total protein and its constituents (including albumin and globulin) declined significantly in the BPH control compared to the normal control. Administration of CEESH to the prostate enlarged rats lowered AST, ALT, and ALP activities in groups 4–5 rats significantly (P<0.05) compared to BPH control. Besides, the CEESH treated had elevated total protein (including albumin and globulin) levels compared to the BPH control. The high bilirubin levels in the BPH control were significantly diminished in the rats treated with CEESH. No alterations in the liver micrographs of the BPH control and CEESH administered rats were observed.
Conclusion: These findings showed that CEESH confers hepatoprotection in BPH induced rats and could be used to manage BPH and other hepatic disorders.
Robert Ikechukwu Uroko, Paul Chukwuemaka Nweje-Anyalowu, Chinomso Friday Aaron, Charles Nnanna Chukwu,
Volume 4, Issue 1 (6-2022)
Abstract
Objective: The combined extract of Spermacoce radiata and Hypselodelphys poggeana leaves has been shown to be a potent therapeutic agent against benign prostatic hyperplasia and it is used extensively for BPH and other diseases by local traditional medicine practitioners without any evaluation of its toxicity effects on liver and other vital organs in the body. The study evaluated the hepatoprotective effects of a combined extract of S. radiata and H. poggeana (CEESH) on rats with prostate enlargement (BPH).
Material and Methods: The study had five groups of rats (n = 6), with groups 1 and 2 being the normal and BPH controls, respectively. Groups 3, 4 and 5 were the BPH rats administered Finasteride, 300 and 600 mg/kg CEESH, respectively. Prostate enlargement was induced in the rat by the subcutaneous administration of testosterone injection (5 mg/kg/day for 28 days), while Finasteride and CEESH were respectively administered to the rats orally as therapeutic agents.
Results: Prostate enlargement in the rats elevated AST, ALT and ALP activities in BPH control coupled with a significant rise in the serum bilirubin levels compared to the normal control. The total protein and its constituents (including albumin and globulin) declined significantly in the BPH control compared to the normal control. Administration of CEESH to the prostate enlarged rats lowered AST, ALT, and ALP activities in groups 4–5 rats significantly (P<0.05) compared to BPH control. Besides, the CEESH treated had elevated total protein (including albumin and globulin) levels compared to the BPH control. The high bilirubin levels in the BPH control were significantly diminished in the rats treated with CEESH. No alterations in the liver micrographs of the BPH control and CEESH administered rats were observed.
Conclusion: These findings showed that CEESH confers hepatoprotection in BPH induced rats and could be used to manage BPH and other hepatic disorders.
Robert Ikechukwu Uroko, Chinomso Friday Aaron, Mercylyn Ezinne Uche, Chinedu Aguwamba, Elisha Uko Ogwo, Paul Chukwuemaka Nweje-Anyalowu, Solomon Nnah Ijioma,
Volume 4, Issue 2 (12-2022)
Abstract
Objective: This study evaluated the effect of ethanol extract of Aju Mbaise (EEAM) on sperm morphology, semen quality, sex hormonal levels, gonadosomatic index and testicular histology of Avodart-induced rats.
Material and Methods: The study had normal control, Avodart control, EEAM control, Avodart+500mg/kg EEAM, and Avodart +1000mg/kg EEAM groups. The rats were orally administered 0.5 mg/kg Avodart and 1 hour later treated with EEAM for 28 consecutive days. The Avodart control had milky white semen with low Ph, volume and semen consistency compared to the normal control and EEAM treated rats with creamy white semen with higher pH and semen consistency, respectively.
Results: The results indicated a substantial decline in spermatozoa mass motility; spermatozoa live proportion, spermatozoa concentrations, normal spermatozoa proportions, animal live weight, paired testes weight and relative testicular weight in the Avodart control compared to the normal control. The Avodart induction caused a significant reduction in the serum testosterone, follicle-stimulating hormone and luteinising hormone levels compared to the normal control. Also, there was a substantial decline in spermatogenic activity and the absence of mature spermatocytes in the lumen of the seminiferous tubules in the Avodart control relative to the normal control and EEAM control. The alterations in the sperm morphology, semen quality, sex hormonal levels and gonadosomatic index were significantly reverted to normal in the EEAM treated Avodart induced rats in a dose-dependent manner compared to the Avodart control.
Conclusion: These findings showed that EEAM ameliorates Avodart toxicity and improves fertility by restoring sperm quality and sex hormonal levels to normal in rats.
