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Uroko R I, Nweje-Anyalowu P C, Aaron C F, Chukwu C N. Combined Extract of Spermacoce radiata (DC.) Hiern and Hypselodelphys poggeana (K.Schum.) Milne-Redh leaves (CEESH) confer Hepatoprotection in Rat Induced Benign Prostatic Hyperplasia. pbp 2022; 4 (1) :68-78
URL: http://pbp.medilam.ac.ir/article-1-115-en.html
URL: http://pbp.medilam.ac.ir/article-1-115-en.html
Robert Ikechukwu Uroko * 
1, Paul Chukwuemaka Nweje-Anyalowu2 , Chinomso Friday Aaron3 , Charles Nnanna Chukwu4
1, Paul Chukwuemaka Nweje-Anyalowu2 , Chinomso Friday Aaron3 , Charles Nnanna Chukwu4
1- Department of Biochemistry, College of Natural Sciences, Michael Okpara University of Agriculture, Umudike, Abia State, Nigeria , ir.uroko@mouau.edu.ng
2- Department of Biochemistry, Faculty of Science, Clifford University, Owerrinta, Abia State, Nigeria
3- Department of Biochemistry, Faculty of Biological and Physical Sciences, Abia State University, Uturu, Nigeria
4- Department of Biochemistry, College of Natural Sciences, Michael Okpara University of Agriculture, Umudike, Abia State, Nigeria
2- Department of Biochemistry, Faculty of Science, Clifford University, Owerrinta, Abia State, Nigeria
3- Department of Biochemistry, Faculty of Biological and Physical Sciences, Abia State University, Uturu, Nigeria
4- Department of Biochemistry, College of Natural Sciences, Michael Okpara University of Agriculture, Umudike, Abia State, Nigeria
Abstract: (1323 Views)
Objective: The combined extract of Spermacoce radiata and Hypselodelphys poggeana leaves has been shown to be a potent therapeutic agent against benign prostatic hyperplasia and it is used extensively for BPH and other diseases by local traditional medicine practitioners without any evaluation of its toxicity effects on liver and other vital organs in the body. The study evaluated the hepatoprotective effects of a combined extract of S. radiata and H. poggeana (CEESH) on rats with prostate enlargement (BPH).
Material and Methods: The study had five groups of rats (n = 6), with groups 1 and 2 being the normal and BPH controls, respectively. Groups 3, 4 and 5 were the BPH rats administered Finasteride, 300 and 600 mg/kg CEESH, respectively. Prostate enlargement was induced in the rat by the subcutaneous administration of testosterone injection (5 mg/kg/day for 28 days), while Finasteride and CEESH were respectively administered to the rats orally as therapeutic agents.
Results: Prostate enlargement in the rats elevated AST, ALT and ALP activities in BPH control coupled with a significant rise in the serum bilirubin levels compared to the normal control. The total protein and its constituents (including albumin and globulin) declined significantly in the BPH control compared to the normal control. Administration of CEESH to the prostate enlarged rats lowered AST, ALT, and ALP activities in groups 4–5 rats significantly (P<0.05) compared to BPH control. Besides, the CEESH treated had elevated total protein (including albumin and globulin) levels compared to the BPH control. The high bilirubin levels in the BPH control were significantly diminished in the rats treated with CEESH. No alterations in the liver micrographs of the BPH control and CEESH administered rats were observed.
Conclusion: These findings showed that CEESH confers hepatoprotection in BPH induced rats and could be used to manage BPH and other hepatic disorders.
Material and Methods: The study had five groups of rats (n = 6), with groups 1 and 2 being the normal and BPH controls, respectively. Groups 3, 4 and 5 were the BPH rats administered Finasteride, 300 and 600 mg/kg CEESH, respectively. Prostate enlargement was induced in the rat by the subcutaneous administration of testosterone injection (5 mg/kg/day for 28 days), while Finasteride and CEESH were respectively administered to the rats orally as therapeutic agents.
Results: Prostate enlargement in the rats elevated AST, ALT and ALP activities in BPH control coupled with a significant rise in the serum bilirubin levels compared to the normal control. The total protein and its constituents (including albumin and globulin) declined significantly in the BPH control compared to the normal control. Administration of CEESH to the prostate enlarged rats lowered AST, ALT, and ALP activities in groups 4–5 rats significantly (P<0.05) compared to BPH control. Besides, the CEESH treated had elevated total protein (including albumin and globulin) levels compared to the BPH control. The high bilirubin levels in the BPH control were significantly diminished in the rats treated with CEESH. No alterations in the liver micrographs of the BPH control and CEESH administered rats were observed.
Conclusion: These findings showed that CEESH confers hepatoprotection in BPH induced rats and could be used to manage BPH and other hepatic disorders.
Keywords: Spermacoce radiata, Hypselodelphys poggeana, benign prostatic hyperplasia, hepatic disorder, hepatic enzymes, liver histomorphology.
Type of Study: Research |
Subject:
Herbal Drugs
Received: 2022/05/1 | Accepted: 2022/05/10 | Published: 2022/05/10
Received: 2022/05/1 | Accepted: 2022/05/10 | Published: 2022/05/10
References
1. Lee C, Kozlowski JM, Grayhack JT. (1995). Etiology of benign prostatic hyperplasia. Urol Clin North Am 1995; 22(2): 237-246.
2. Briganti A, Capitanio U, Suardi N, Gallina A, Salonia A, Bianchi M, et al. Benign prostatic hyperplasia and its aetiologies. Eur Urol Suppl 2009; 8(13): 865-871.
3. Akbari F, Azadbakht M, Megha K, Dashti A., Vahedi L, Nejad A B, et al. Evaluation of Juniperus communis L. seed extract on benign prostatic hyperplasia induced in male Wistar rats. Afr J Urol 2021; 2021: 27:48.
4. Rohrmann S, Katzke V, Kaaks R. Prevalence and progression of lower urinary tract symptoms in an aging population. Urol 2016; 95: 158–163.
5. Iscaife A, Anjos G, Barbosa NC, Nahas WC, Srougi M, Antunes AA. The role of bladder diverticula in the prevalence of acute urinary retention in patients with BPH who are candidates to surgery. Int Braz J Urol 2018; 44(4):765-770.
6. Keong TF. Pathophysiology of clinical benign prostatic hyperplasia. Am Res J Urol 2017; 4: 152-157.
7. Gormley J, Stoner E, Bruskewitz RC, Imperato-McGinley J, Walsh PC, McConnell JD. The effect of Finasteride in men with benign prostatic hyperplasia. N Engl J Med 1992; 327(17): 1185-1191.
8. Yu ZJ, Yan HL, Xu FH, Chao HC, Deng LH, Xu XD, et al. Efficacy and side effects of drugs commonly used for the treatment of lower urinary tract symptoms associated with benign prostatic hyperplasia. Front Pharmacol 2020; 11: 658. https://doi.org/10.3389/fphar.2020.00658
9. Miles CB, Charu T, Lomas KT, Pradeep K, Yahya EC, Viness, P. Nutraceutical-based therapeutics and formulation strategies augmenting their efficiency to complement modern medicine: An Overview. J Funct Foods 2014; 6: 82-99.
10. Pereira ZV, Carvalho-Okano RM, Garcia FC. Rubiaceae Juss. da Reserva Florestal Mata do Paraíso, Viçosa, MG, Brasil. Acta Bot Brasil 2006; 20:207-224.
11. Vadivelan S, Sinha BN, Betanabhatla KS, Christina AJ, Pillai RN. Anti-inflammatory activity of Spermacoce articularis Linn on carrageenan induced paw edema in Wistar male rats. Pharmacolonline 2007; 3: 478-484.
12. Subramanya MD, Pai SR, Upadhya V, Ankad GM, Bhagwat SS, Hegde HV. Total polyphenolic contents and in vitro antioxidant properties of eight Sida species from Western Ghats, India. J Ayurveda Integr Med 2015; 6: 24‑8.
13. Abbiw D. Useful Plants of Ghana: West African Uses of Wild and Cultivated Plants London: Intermediate Technology Publications, Royal Botanic Gardens, Kew 1990. p. 337.
14. Uroko RI, Anyiam PN, Uhuo EN, Ajah O. Combined ethanol extract of Spermacoce radiata and Hypselodelphyspoggeana prevents renal damage and dyslipidemia in benign prostatic hyperplasia induced rats. J Herb Med 2021; 12(4): 43-52.
15. Thornburg JM. Targeting aspartate aminotransferase in breast cancer. Breast Cancer Res 2008; 10(5): 84-84.
16. Ekeyi Y, Uchendu NO, Anaduaka EG, Ezeanyika LUS. Ethanol extract of Cassia sieberiana leaves ameliorates deviances associated with benign prostatic hyperplasia in rats. All Life 2021; 14: 473-483.
17. Chukwu CN, Uroko RI, Egba SI, Adamude FA, Asadu CL. Effects of combined ethanol extract of Anthocleista Vogelii and Alstonia Boonei stem barks on liver function indices in benign prostatic hyperplasia induced rats. Nigerian J Pharm Res 2021; 16(2):191-201.
18. Pratt DS, Kaplan MM. Evaluation of abnormal liver-enzyme results in asymptomatic patients. Acta Clin Belg 2004; 59(5): 285-289.
19. Stocken DD. Modelling prognostic factors in advanced pancreatic cancer. Br J Cancer 2008; 99(6): 883-893.
20. Amang AP, Kodji E, Mezui C, Baane MP, Siwe GT, Kuissu TM, Emakoua J, Tan PV. Hepatoprotective Effects of Aqueous Extract of Opilia celtidifolia (Opiliaceae) Leaves against Ethanol-Induced Liver Damage in Rats. Evid Based Complement Alternat Med 2020; 2020:6297475.
21. Tofik AE, Zawdie B, Nair SKP, Welde M, Mateos HT. Evaluation of the effect of hydromethanolic seed extract of Lepidium sativum L. (Fetto) on deep-fried palm oil diet induced nonalcoholic fatty liver disease on male Swiss albino mice. Hepatic Medicine: Evid Res 2022; 14: 1-12.
22. Chong CLG, Hussan F, Othman F. Hepatoprotective effects of Morinda citrifolia leaf extract on ovariectomized rats fed with thermoxidized palm oil diet: evidence at histological and ultrastructural level. Oxid Med Cell Longev 2019; 20(2): 2-10.
23. Nawaz H, Rehman T, Aslam M, Kiran S, Feen T, Nawaz M. Optimization of Phyllanthus emblica L. leaf extract-assisted clearance of hyperbilirubinemia in White New Zealand albino rabbits. All Life 2022; 15:54-63.
24. Gumaih HS. (2015). Effect of reused palm oil on biochemical and haematological parameters of mice. Egypt Acad J Biol Sci 2015; 7(1):13-21.
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