Volume 4, Issue 2 (12-2022)
pbp 2022, 4(2): 43-47 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
T M, Y.I. M, H A, A. J Y. Isolation and Characterization of Kaempferol from Tapinanthus globiferus Growing on Balanites aegyptiaca. pbp 2022; 4 (2) :43-47
URL: http://pbp.medilam.ac.ir/article-1-141-en.html
URL: http://pbp.medilam.ac.ir/article-1-141-en.html
1- Department of Chemistry, Sokoto State University, Sokoto Nigeria
2- Department of Chemistry, Sokoto State University, Sokoto Nigeria ,Y.I.mansur@ymail.com
3- Department Pharmaceutical and Medicinal Chemistry, Usmanu Danfodoyo University, Sokoto, Nigeria
2- Department of Chemistry, Sokoto State University, Sokoto Nigeria ,
3- Department Pharmaceutical and Medicinal Chemistry, Usmanu Danfodoyo University, Sokoto, Nigeria
Abstract: (2081 Views)
Objective: Tapinanthus globiferus is a hemi-parasitic plant used in ethonomedicine to treat different ailment including fungal infections. The aim of the study was to isolation the bioactive compound from leaf of T.globiferus growing on Balanites aegyptiaca.
Material and Methods: The plant material was collected, identified pulverized to powder using mortar and pestle. The powdered plant material was subjected to maceration with 90% methanol to obtained crude methanol leaf extract which was further partitioned with solvent of increasing polarity to obtained n-hexane, chloroform, ethylacetate and n-butanol fractions.
Results: Chromatographic separation of ethylacetate fraction using a combination of silica gel column and sephadex LH-20 column led to the isolation of flavonoid (3,5,7- trihydroxy-2-(4-hydroxyphenyl)-4H-chromen-4-one). The structure of the compound obtained was established by Spectroscopic analysis (FTIR, NMR).
Conclusion: Chromatographic studies of ethyl acetate fraction of T. globiferus growing on Balanites aegyptiaca, afforded a 3,5,7-trihydroxy-2-(4-hydroxyphenyl)-4H-chromen-4-one.To the best of our knowledge, this is the first report of isolation of this compound from Tapinanthus globiferus growing on Balanites aegyptiaca.
Material and Methods: The plant material was collected, identified pulverized to powder using mortar and pestle. The powdered plant material was subjected to maceration with 90% methanol to obtained crude methanol leaf extract which was further partitioned with solvent of increasing polarity to obtained n-hexane, chloroform, ethylacetate and n-butanol fractions.
Results: Chromatographic separation of ethylacetate fraction using a combination of silica gel column and sephadex LH-20 column led to the isolation of flavonoid (3,5,7- trihydroxy-2-(4-hydroxyphenyl)-4H-chromen-4-one). The structure of the compound obtained was established by Spectroscopic analysis (FTIR, NMR).
Conclusion: Chromatographic studies of ethyl acetate fraction of T. globiferus growing on Balanites aegyptiaca, afforded a 3,5,7-trihydroxy-2-(4-hydroxyphenyl)-4H-chromen-4-one.To the best of our knowledge, this is the first report of isolation of this compound from Tapinanthus globiferus growing on Balanites aegyptiaca.
Type of Study: Research |
Subject:
Herbal Drugs
Received: 2022/07/17 | Accepted: 2022/12/1 | Published: 2022/12/1
Received: 2022/07/17 | Accepted: 2022/12/1 | Published: 2022/12/1
References
1. Abubakar, H. Musa, A.M. Abdullahi, M.I. Mzozoyana, V. Yusuf, A.J .Isolation and characterization of some flavonoids from the leaf of Tapinanthus globiferus growing on Vitex doniana. Brazilian Journal of Biological Sciences 2020;7(16): 239-245. DOI: https://doi.org/10.22270/ujpr.v5i1.361
2. Abubakar, K. Adebisi, I.M. and Ugwah, J.C. Idiris, G.O Mshelia,H.E. Phytochemical Screening and Anticonvulsant Activity of the Residual Aqueous Fraction of Tapinanthus globiferus growing on Ficus glums. Herbal Medicine 2016;2 (2). 13
3. Bassey, M.E. Phytochemical investigation of Tapinanthus globiferus from two hosts and the taxonomic implication. International Journal of Chemical Environmental and Pharmaceutical Research 2012; 3(2): 174-177. ISSN; 2229-5283
4. Celestine, J. Umar, A.K. Aliyu, N. Sherifat, B.A. Mustapha, A.I. Hadiza, D.N.. Phytochemical Screening and Anti-Inflammatory Studies of Tapinanthus globiferus (A. Rich) Teigh. Leaves Three Extracts. Journal of Pharmaceutical Sciences 2019; 25(2): 124-131 DOI: 10.15171/PS.2019.19
5. Burkill, H.M.. Useful Plants of West Tropical Africa. Vol.5 2nd edition Royal Botanic Gardens, Kew England. 2000; 548-560.
6. Adesina, S.K., Illoh, H.C., Johnny, II. and Jacob I. E. African mistletoe (loranthaceae); Ethnopharmacology, chemistry and medicinal values: An update. African Journal of Traditional, Complementary and Alternative Medicine 2013; 10(4): 161-170. DOI.org/10.4314/ajtcam.v10i4.26
7. Yetunde O, Mohammad I, Olusola, O. Elekofehinti, A. Adeniran, A. Olalekan, A. J. Batista, T.R, and Jean, P. K. .Journal of Intercultural Ethnopharmacology2014;3(4):167–172.DOI: 10.5455/jice.20140826110059
8. Muhammad, .K. B. Sani, M.D. Mohammed, M.L. Hassan, H. Abubakar, A.M. Mubarak, B.D. and Amina, J.Y.. Isolation and characterization of some flavonoids from the leaf of Tapinanthus globiferus growing on Acacia nilotica. Caliphate Journal of Science & technology (CaJoST), 2022; 4(1): 40-45. DOI: https://dx.doi.org/10.4314/cajost.v4i1.9
9. Mukhtar, T. Hassan. A, and Amina, J. Y. Phytochemical and Antifungal Studies of Tapinanthus globiferus Extract and Fractions. Caliphate Journal of Science & technology (CaJoST) 2022; 4(1):71-77. DOI: https://dx.doi.org/10.4314/cajost.v4i1.9
10. Okpako, L.C and Ajaiyeoba E.O. In vitro and in vivo antimalarial studies of Striga hermonthica and Tapinanthus sessilifolius extracts. African Journal of Medicine and Medical Sciences, 2004; 33 (1):73-75 PMID:15490799.
11. Adekunle, A.S. Oyewo, B.E. and Afolabi, O.K.. Therapeutic efficacy of Tapinanthus globiferus on acetaminophen induced nephrotoxicity, inflammatory reactions and oxidative stress in albino rats. International research Journal of Biochemistry and Bioinformatics. 2012; 2(2): 041-045. SSN-2250-9941
12. Sylvin Ouédraogo; Aristide Traoré; N. Somé; M. Lompo; Pierre I. Guissou; Christa Schott Bernard Bucher; Ramaroson Andriantsitohaina. African Journal of Traditional, Complementary and Alternative Medicines 2005; 2(1). 21-30. ISSN 0189-6016
13. Bakkialakshmi, S. and Jayoti R.. Infrared spectrum analysis of some flavonoids with hemoglobin. International Journal of Applied and Advanced Scientific Research (IJAASR) 2017; 2(2): 107-110. ISSN : 2456 – 3080.
14. Sathyadevi, M. and Subramanian, S.. extraction, isolation and characterization of bioactive flavonoids from the Fruits of Physalis Peruviana Linn Extract. Asian Journal of Pharmaceutical and Clinical Research 2015; (8):1, 152-157. ISSN - 0974-2441
15. kadiri, S. K Avanapu S. R. Extraction, isolation and structural elucidation of flavonoid from chrozophora plicata leaves and evaluation of its antioxidative potentials asian journal of pharmaceutical and clinical research.2017;4(10).460-469.DOI: doi.org/10.22159/ajpcr.2017.v10i4.17106
16. Yu-Lan,Li.Jun Li, Nai Li, W. and Xin-Sheng Y.A.O.. Flavonoids and a New Polyacetylene from Bidens parviflora Willd 2008; 1938-1939. DOI: 10.3390/molecules13081931
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |