Volume 5, Issue 2 (12-2023)
pbp 2023, 5(2): 122-130 |
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Zangeneh M M, Etemadi N, Zand S, Pirnejad-Talatapeh A, Zangeneh A, Abangah G et al . The effect of silver nanoparticles of aqueous Matricaria chamomilla extract on acute liver toxicity caused by acetaminophen in mice. pbp 2023; 5 (2) :122-130
URL: http://pbp.medilam.ac.ir/article-1-203-en.html
URL: http://pbp.medilam.ac.ir/article-1-203-en.html
Mohammad Mehdi Zangeneh1 
, Navid Etemadi2 , Samira Zand3 , Ali Pirnejad-Talatapeh4 , Akram Zangeneh * 
5, Ghobad Abangah6 , Hori Ghaneialvar1
, Navid Etemadi2 , Samira Zand3 , Ali Pirnejad-Talatapeh4 , Akram Zangeneh * 5, Ghobad Abangah6 , Hori Ghaneialvar1
1- Biotechnology and Medicinal plants Research Center Ilam University of Medical Sciences,Ilam, Iran
2- DVM Graduate, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
3- Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
4- Central Laboratory, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
5- Biotechnology and Medicinal plants Research Center Ilam University of Medical Sciences,Ilam, Iran , azangeneh@ymail.com
6- School of Medicine, Shahid Mostafa Khomaeini Hospital, Ilam University of Medical Sciences, Ilam, Iran
2- DVM Graduate, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
3- Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
4- Central Laboratory, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
5- Biotechnology and Medicinal plants Research Center Ilam University of Medical Sciences,Ilam, Iran , azangeneh@ymail.com
6- School of Medicine, Shahid Mostafa Khomaeini Hospital, Ilam University of Medical Sciences, Ilam, Iran
Abstract: (429 Views)
Objective: Attention to the daily prescription of large amounts of drugs by doctors; In recent years, much attention has been paid to the effect of plants in the treatment and prevention of drug poisoning. Matricaria chamomilla is one of the oldest medicinal plants known to man and its use dates back to ancient Greece. In this research, the protective effect of green tea extract in hepatotoxicity caused by acetaminophen has been investigated.
Methods: So that 48 adult male mice were randomly divided into 6 groups. The control group received only physiological serum. The M. chamomile group received 250 mg/kg and (AgNP)M chamomilla received 0.5 mg/kg for 30 days. The acetaminophen group was prescribed 500 mg/kg of acetaminophen orally, and the experimental groups (acetaminophen + M. chamomile extract) were given chamomile extract with a dose of 250 mg/kg and (silver nanoparticle of M. chamomile plant + acetaminophen) (AgNP) M. chamomilla with a dose of 0.5 received mg/kg for 30 days and a toxic dose of acetaminophen was prescribed on the 29th day.
Results: On the 31st day, blood was drawn to measure alanine transferase (ALT) and aspartate transferase (AST), and after that, the animals' livers were placed in 10% formalin for histopathological examination. The serum levels of ALT and AST enzymes in the silver nanoparticle group were significantly reduced compared to other groups. (P<0.05) so that in histopathological studies, liver necrosis, congestion of red blood cells and the accumulation of inflammatory cells in (AgNP) M. chamomilla were reduced compared to the acetaminophen group.
Conclusion: According to the results of this research (AgNP)M chamomilla is probably involved in liver necrosis caused by acetaminophen. It has a protective role.
Methods: So that 48 adult male mice were randomly divided into 6 groups. The control group received only physiological serum. The M. chamomile group received 250 mg/kg and (AgNP)M chamomilla received 0.5 mg/kg for 30 days. The acetaminophen group was prescribed 500 mg/kg of acetaminophen orally, and the experimental groups (acetaminophen + M. chamomile extract) were given chamomile extract with a dose of 250 mg/kg and (silver nanoparticle of M. chamomile plant + acetaminophen) (AgNP) M. chamomilla with a dose of 0.5 received mg/kg for 30 days and a toxic dose of acetaminophen was prescribed on the 29th day.
Results: On the 31st day, blood was drawn to measure alanine transferase (ALT) and aspartate transferase (AST), and after that, the animals' livers were placed in 10% formalin for histopathological examination. The serum levels of ALT and AST enzymes in the silver nanoparticle group were significantly reduced compared to other groups. (P<0.05) so that in histopathological studies, liver necrosis, congestion of red blood cells and the accumulation of inflammatory cells in (AgNP) M. chamomilla were reduced compared to the acetaminophen group.
Conclusion: According to the results of this research (AgNP)M chamomilla is probably involved in liver necrosis caused by acetaminophen. It has a protective role.
Received: 2023/10/31 | Accepted: 2023/12/1 | Published: 2023/12/1
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