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Bahmani M, Kazemnezhad M, Karimi E, Ghaneialvar H. Comprehensive Phytochemical Profiling of Native Cannabis sativa L. from Dehloran Using HS-SPME, GC–MS, and FT-IR Techniques. pbp 2026; 8
URL: http://pbp.medilam.ac.ir/article-1-377-en.html
URL: http://pbp.medilam.ac.ir/article-1-377-en.html
1- Ilam University of Medical Sciences, Biotechnology and Medicinal Plants Research Center, Ilam, Iran
2- Ilam University of Medical Sciences, Biotechnology and Medicinal Plants Research Center, Ilam, Iran ,ghaneialvar@gmail.com
2- Ilam University of Medical Sciences, Biotechnology and Medicinal Plants Research Center, Ilam, Iran ,
Abstract: (254 Views)
Objective: Cannabis sativa, a member of the Cannabaceae family, has a long history of use in Iranian traditional medicine, particularly for pain relief, alleviating menstrual discomfort, and improving mood disorders. This study aimed to extract and characterize the phytochemical constituents of native C. sativa from Dehloran (Ilam Province) using headspace solid-phase microextraction (HS-SPME), gas chromatography-mass spectrometry (GC-MS), and Fourier-transform infrared (FT-IR) analytical techniques.
Methods: Plant specimens were collected from Dehloran, located in southern Ilam Province. The aerial parts of the plants were shade-dried and finely powdered. Essential oils were extracted using HS-SPME, and their chemical constituents were analyzed using GC-MS. FT-IR spectroscopy was employed to identify key functional groups. The essential oil extracted from Dehloran C. sativa seeds using HS-SPME was successfully analyzed with GC-MS.
Results: A total of 36 volatile compounds were identified, with β-pinene, trans-caryophyllene, and α-humulene as the primary constituents. The chemical profile included monoterpenes, sesquiterpenes, oxygenated and esterified terpenoids, as well as several non-terpenoid compounds. FT-IR analysis further validated the presence of key functional groups, such as alcohols, esters, carbonyls, and alkanes. These findings collectively emphasize the plant's richness in bioactive and antioxidant compounds, highlighting its potential as a valuable resource for pharmaceutical applications.
Conclusion: The essential oil of native C. sativa is rich in bioactive and antioxidant constituents, making it a promising candidate for developing medicinal formulations. The findings of this study provide a foundation for identifying pharmacologically relevant compounds in indigenous Iranian cannabis ecotypes and may aid in the advancement of natural therapeutic products.
Methods: Plant specimens were collected from Dehloran, located in southern Ilam Province. The aerial parts of the plants were shade-dried and finely powdered. Essential oils were extracted using HS-SPME, and their chemical constituents were analyzed using GC-MS. FT-IR spectroscopy was employed to identify key functional groups. The essential oil extracted from Dehloran C. sativa seeds using HS-SPME was successfully analyzed with GC-MS.
Results: A total of 36 volatile compounds were identified, with β-pinene, trans-caryophyllene, and α-humulene as the primary constituents. The chemical profile included monoterpenes, sesquiterpenes, oxygenated and esterified terpenoids, as well as several non-terpenoid compounds. FT-IR analysis further validated the presence of key functional groups, such as alcohols, esters, carbonyls, and alkanes. These findings collectively emphasize the plant's richness in bioactive and antioxidant compounds, highlighting its potential as a valuable resource for pharmaceutical applications.
Conclusion: The essential oil of native C. sativa is rich in bioactive and antioxidant constituents, making it a promising candidate for developing medicinal formulations. The findings of this study provide a foundation for identifying pharmacologically relevant compounds in indigenous Iranian cannabis ecotypes and may aid in the advancement of natural therapeutic products.
Keywords: Cannabis sativa, Fourier Transform Infrared Spectroscopy, Gas Chromatography–Mass Spectrometry, Headspace Solid-Phase Microextraction, Dehloran ecotype, Iran
Type of Study: Research |
Subject:
Herbal Drugs
Received: 2025/05/9 | Accepted: 2025/12/5 | Published: 2026/01/1
Received: 2025/05/9 | Accepted: 2025/12/5 | Published: 2026/01/1
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