Volume 3, Issue 1 (6-2021)                   Plant Biotechnol Persa 2021, 3(1): 9-17 | Back to browse issues page


XML Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Abbasi N, ghaneialvar H, Saneei S, zangeneh M M, zangeneh A. Assessment of the Wound Healing Property of Aqueous Extract of Stevia Rebaudiana: an Ethnomedicinal Plant. Plant Biotechnol Persa 2021; 3 (1) :9-17
URL: http://pbp.medilam.ac.ir/article-1-61-en.html
Biotechnology and Medicinal Plants Research Center, Ilam University of Medical Sciences, Ilam, Iran , m.mehdizangeneh@yahoo.com
Abstract:   (1078 Views)
Ethnomedicinal plants are considered as the recent resources for producing components to healt wounds. Stevia rebaudiana is a native plant in Iran, which has been used as an anti-inammatory, antioxidant, antibacterial, antifungal, antiparasitic, antiviral, and antidiabetic agent. In this study, the authors describe the property of aqueous extract of S. rebaudiana on wound healing markers in Sprague-Dawley male rats. Full-thickness excisional wounds (2×2 cm) was induced on the back of 32 rats. In this study, rats were randomly divided into four main groups (n=8), untreated (control) and treated with 1ml basal cream, 1ml tetracycline (3%), and 1ml S. rebaudiana aqueous extract 10% for 10 days. Animals of each group were euthanized at 10 days post-injury (DPI) and wounds were assessed through gross and histopathological analyses. Treated animals with S. rebaudiana demonstrated a considerable decrease in the wound area during the experiment compared to control, basal cream, and tetracycline groups. Parameters such as healing tissue alignment and epithelialization indicated substantial changes when compared to other groups. Moreover, treatment with S. rebaudiana decreased the number of wound surface area, lymphocytes, and macrophages, it increased the number of blood vessels and fibrocytes compared to other groups at 10 days. Thus, the present research shows the wound healing effect of the S. rebaudiana, suggesting to be used as a therapeutic supplement. Additional in vivo researches and clinical trials would be needed to justify the findings.
 
Full-Text [PDF 2966 kb]   (109 Downloads)    
Type of Study: Research | Subject: Herbal Drugs
Received: 2020/12/19 | Accepted: 2021/01/5 | Published: 2021/07/6

References
1. Chitra S, Patil MB and Ravi K. Wound healing activity of Hyptis suaveolens (L) poit (Laminiaceae). Int J Pharm Technol Res. 2009; 1(3): 737-44.
2. Falanga V. Wound healing and its impairment in the diabetic foot. Lancet. 2005; 12: 1736-43.
3. Hagh-Nazari L, Goodarzi N, Zangeneh MM, Zangeneh A, Tahvilin R and Moradi R. Stereological study of kidney in streptozotocin-induced diabetic mice treated with ethanolic extract of Stevia rebaudiana (bitter fraction). Comp ClinPathol. 2017; 26 (2): 455–63.
4. Buganza-Tepole A and Kuhl E. Systems-based approaches toward wound healing. Pediatr Res. 2013; 73(4): 553-63.
5. Singh MP and Sharma CS. Wound healing activity of Terminalia Chebula in experimentally induceddiabetic rats. Int J Pharm Technol Res. 2009; 4(1): 1267-70.
6. Faramarzi E, Zangeneh MM, Zangeneh A and Moradi R. Effect of Cinnamomum zelanicumon oil on hyponeophagia anxiety test in Balb C male mice. Onl J Vet Res. 2017; 21(2): 77-80.
7. Foroughi A, Pournaghi P, Najafi F, Zangeneh A, Zangeneh MM and Moradi R. Antibacterial effect and phytochemical screening of essential oil of Pimpinella anisum against Escherichia coli O157:H7 and Staphylococcus aureus. Int J Curr Med Pharm Res. 2016; 7(6): 367-71.
8. Ghashghaii A, Hashemnia M, Nikousefat Z, Zangeneh MM, Zangeneh A. Wound healing potential of methanolic extract of Scrophularia striata in rats. Pharm Sci. 2017; 23 (4): 256-63.
9. Zangeneh MM, Goodarzi N, Zangeneh A, Tahvilian R, Najafi F. Amelioration of renal structural changes in STZ-induced diabetic mice with ethanolic extract of Allium saralicum R.M. Fritsch. Comp Clin Pathol. 2018; 27: 861-867.
10. Zangeneh MM, Zangeneh AA, Tahvilian R, Moradi R. Evaluation of the nephroprotective effect of Glycyrrhiza glabra L aqueous extract on CCl4-induced nephrotoxicity in mice. Comp Clin Pathol. 2018; 27:1119-1126.
11. Foroughi A, Pournaghi P, Najafi F, Zangeneh A, Zangeneh MM and Moradi R. Medicinal Plants: Antibacterial Effects and Chemical Composition of Essential Oil of Foeniculum vulgare. Int J Curr Pharm Res. 2017; 8(1): 13-7.
12. Mohammadi G, Zangeneh MM, Zangeneh A, Siavash-Haghighi ZM. Chemical characterization and anti‐breast cancer effects of silver nanoparticles using Phoenix dactylifera seed ethanolic extract on 7,12‐Dimethylbenz[a] anthracene‐induced mammary gland carcinogenesis in Sprague Dawley male rats. Appl. Organomet. Chem. 2020; 34(1): e5136.
13. Baie SH and Sheikh KA. The wound healing properties of Channastriatus-cetrimide creamwound contraction and glycosaminogly canmeasurement. J Ethnopharmacol 2000; 73(1): 15-30.
14. Nayak BS, Isitor G, Davis EM and Pillai GK. The Evidence based wound healing activity of lawsoniainermis Linn. Phytother Res. 2007; 21(9): 827-31.
15. Ohshima H, Yoshie Y, Auriol S and Gilibert I. Antioxidant and pro-oxidant actions of flavonoids: effects on DNA damage induced by nitric oxide, peroxynitrite and nitroxyl anion. Free Radic Biol Med 1998; 25(9): 1057-65.
16. Joshi A, Joshi VK, Pandey D, Hemalatha S. Systematic investigation of ethanolic extract from Leea macrophylla: implications in wound healing. J Ethnopharmacol. 2016; 191: 95–106, 2016. (b) Yariswamy M, Shivaprasad HV, Joshi V, Nanjaraj Urs AN, Nataraju A, Vishwanath BS. Topical application of serine proteases from Wrightia tinctoria R. Br. (Apocyanaceae) latex augments healing of experimentally induced excision wound in mice. J Ethnopharmacol. 2013: 149: 377–383 (c) de Castro Campos Pinto N, Cassini-Vieira P, de Souza-Fagundes EM, Barcelos LS, Castañon MSMN, Scio E, Pereskia aculeata Miller leaves accelerate excisional wound healing in mice. J Ethnopharmacol. 2016; 194: 131–136. (d) dos Santos Gramma LS, Marques FM, Vittorazzi C, Struthanthus vulgaris ointment prevents an over expression of inflammatory response and accelerates the cutaneous wound healing. J Ethnopharmacol, 2016; 190: 319–327. (e) Biswas TK, Pandit S, Chakrabarti S, Banerjee S, Poyra N, Seal T. Evaluation of Cynodon dactylon for wound healing activity. J Ethnopharmacol. 2017; 197: 128–137. (16f) Bhat PB, Hegde S, Upadhya V, Hegde GR, Habbu PV, Mulgund GS. Evaluation of wound healing property of Caesalpinia mimosoides Lam. J Ethnopharmacol. 2016; 193: 712–724.
17. Afshar M, Sattari fard H, Shadi M and Ghaderi R. Repairing effects of Iran flora on wound healing. J Birjand Univ Med Sci. 2015; 22 (1):1-18.
18. Bakhtiyari Z and Radan MR. Pharmacological effects of medicinal plants on skin. Dermatology and Cosmetic, 2013; 4 (2): 98-106.
19. Zangeneh MM, Najafi F, Moradi R, Tahvilian R, Haghnazari L and Zangeneh A. Evaluation of the in vitro antibacterial activities of alcoholic extract of Stevia rebaudiana against Escheriachia coli O157: H7 (ATCC No. 25922). Asian J Pharm Anal Med Chem. 2016; 4(3): 131- 136.
20. Sherkatolabbasieh H, Hagh-Nazari L, Shafiezadeh S, Goodarzi N, Zangeneh MM, Zangeneh A. Ameliorative effects of the ethanolic extract of Allium saralicum R.M. Fritsch on CCl4-induced nephrotoxicity in mice: A stereological examination. Arch Biol Sci. 2017; 69(3):535-43.
21. Hemmati S, Rashtiani A, Zangeneh MM, Mohammadi P, Zangeneh A, Veisi H. Green synthesis and characterization of silver nanoparticles using Fritillaria flower extract and their antibacterial activity against some human pathogens. Polyhedron 2019; 158: 8–14.
22. Zangeneh MM. Hematoprotective and nephroprotective properties of ethanolic extract of Anthemis odontostephana Boiss in streptozotocin-induced diabetic mice. J Med Plants 2018; 17(65): 47-60.
23. Oryan A, Mohammadalipour A, Moshiri A, Mohammad R and Tabandeh. Avocado/soybean unsaponifiables: a novel regulator of cutaneous wound healing, modelling and remodeling. Int Wound J. 2015; 12(6): 674-85.
24. Zangeneh MM, Zangeneh A, Salmani S, Jamshidpour R, Koosari F. Protection of phenylhydrazine-induced hematotoxicity by aqueous extract of Ocimum basilicum in Wistar male rats. Comp Clin Path. 2019; 28: 331-338.
25. Goorani S, Shariatifar N, Seydi N, Zangeneh A, Moradi R, Tari B, Nazari F, Zangeneh MM. The aqueous extract of Allium saralicum R.M. Fritsch effectively treat induced anemia: experimental study on Wistar rats. Orient Pharm Exp Med 2019; 19: 403-413.
26. Tahvilian R, Moradi R, Zhaleh H, Zangeneh MM, Zangeneh A, Yazdani H, Hajialiani M. Evaluation of Antibacterial properties of essential oil of Pistacia khinjuk. Int J Res Pharm Sci. 2017; 7(1): 1-6.
27. Foroughi A, Zangeneh MM, Kazemi N and Zangeneh A An in vitro study on antimicrobial properties of Allium noeanumreut ex regel: An ethnomedicinal plant. Iranian J Publ Health 2016; 45(2): 32.
28. Zangeneh A, Zangeneh MM, Goodarzi N, Najafi F, Hagh-Nazari L. Protective effects of aqueous extract of internal septum of walnut fruit on diabetic hepatopathy in streptozotocin-induced diabetic mice. Sci J Kurdistan Univ Med Sci. 2018; 23(1): 26-37.
29. Zangeneh MM, Zangeneh A, Amiri H, Amiri N, Tahvilian R, Moradi R, Zhaleh H, Razeghi Tehrani P. Nephroprotective activity of Alyssum meniocoides Boiss aqueous extract on streptozotocin-induced diabetic nephrotoxicity in male mice. Comp Clin Pathol. 2018; 27(5): 1147-1154.
30. Sayyedrostami T, Pournaghi P, Ebrahimi Vosta-Kalaee S, Zangeneh MM. Evaluation of the wound healing activity of Chenopodium botrys leaves essential oil in rats (A short-term study). J Essent Oil Bear Pl. 2018; 21(1): 164-74.
31. Langton AK, Herrick SE and Headon DJ. An extended epidermal response heals cutaneous wounds in the absence of a hair follicle stem cell contribution. J Invest Dermatol 2008; 128(5): 1311.
32. Nayak BS and Pinto Pereira LM. Catharanthus roseus flower extract has wound-healing activity in Sprague Dawley rats. Complement Altern Med. 2006; 21(6): 41-7.
33. Oryan A, Tabatabaieinaieni A, Moshiri A, Mohammadalipoor A and Tabandeh MR. Modulation of cutaneous wound healing by Silymarin in rats. J Wound Care 2012; 21(9): 457-64.
34. Courtois MC, Sapoval M, Del Giudice C, Ducloux R, Mirault T and Messas E. Distal revascularization in diabetic patients with chronic limb ischemia. J Mal Vasc. 2015; 40(1): 24-36.

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.