Volume 5, Issue 1 (6-2023)                   pbp 2023, 5(1): 93-102 | Back to browse issues page


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Yakubu J, Hassan A M, Yesufu H B, Abdulrahman F I, Sodipo O A, Ndai A M et al . Phyto-pharmacological Benefit of Methanol Whole Plant Extract of Ocimum canum Sims. in Chemically-induced Epileptic Rat Model. pbp 2023; 5 (1) :93-102
URL: http://pbp.medilam.ac.ir/article-1-176-en.html
1- Department of Pure and Applied Chemistry, Faculty of Science, University of Maiduguri, PMB, 1069, 1069, Maiduguri, Borno State, Nigeria. , jamesyakubu96@gmail.com
2- Department of Pure and Applied Chemistry, Faculty of Science, University of Maiduguri, PMB, 1069, 1069, Maiduguri, Borno State, Nigeria.
3- Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Maiduguri, PMB, 1069, Maiduguri, Borno State, Nigeria.
4- Department of Pure and Applied Chemistry, Faculty of Science, University of Maiduguri, PMB, 1069, Maiduguri, Borno State, Nigeria
5- Department of Clinical Pharmacology and Therapeutics, Faculty of Basic Clinical Sciences, College of Medical Sciences, University of Maiduguri, Borno State, Nigeria.
6- Department of Pharmaceutical Outcomes and Policy, College of Pharmacy, University of Florida, Florida, USA.
7- Department of Pharmacology, Faculty of Veterinary Medicine, University of Maiduguri, Nigeria
Abstract:   (491 Views)
Objectives: The role of Ocimum canum Sim., as a medicinal agent used for the treatment and management of diseases cannot be overemphasized. The aim of the study is to screen the Phytochemical constituents and to evaluate the antiepileptic effect of methanol whole plant extract in Wistar albino rats.
Material and Methods: The whole plant part of O. canum was extracted with 85% percentage of methanol and screened for phytochemicals. An acute toxicity study was carried out using Lorke’s method and the antiepileptic activity was evaluated using pentylenetetrazole (PTZ) and strychnine in rats. One-way analysis of variance (ANOVA) was used in the analysis of the data.
Results: The phytochemical analysis revealed the presence of saponins, cardiac glycosides, flavonoids, terpenoids, and cardenolides. The intraperitoneal median lethal dose value (LD50) of O. canum in rats was 2154 mg/kg. The extract at the dose of 300 and 600 mg/kg body weight protected 40% and 80% of experimental animals against both PTZ and strychnine-induced convulsion; protected 60% and 80% of rats against death induced by strychnine; compared to 100% protection by the standard drug sodium valproate (20mg/kg).
Conclusion: The antiepileptic investigation suggests that methanol extract of whole plant of O. canum has antiepileptic activity which could be employed for the management of epileptic related conditions
Full-Text [PDF 2397 kb]   (142 Downloads)    
Type of Study: Research | Subject: Pharmacognosy
Received: 2023/03/4 | Accepted: 2023/03/15 | Published: 2023/05/31

References
1. Akinyemi O, Oyewole SO, Jimoh KA. Medicinal plants and sustainable human health: A Review. Hortcult Int J. 2018;2(4):194-195.
3. Dewick PM. Tumor Inhibition from Plants: Trease and Evans Pharmacognosy, 11th Edition, 1996; pp. 45-50.
5. Lucy H, Edgar JD. Medicinal Plants: a re-emerging health aid. Elect J Biotech. 1999;2(2): 7-14.
6. Raza M, Shaheen F, Choudhary MI, Sombati, S, Rafiqa, A, Suria, A, Rahman A, DeLorenzo RJ.
7. Anticonvulsant activities of ethanolic extract and aqueous fraction isolated from Delphinium denudatum. J Ethnopharmacol. 2001;78:73 – 78.
9. Schachter, SC. Evaluation and management of the first Seizure in adults. Up to date. http://www.update.com/contents/search. 2021.
11. WHO. World Health Organization. Epilepsy. https://who.int/news-room/fact-sheets/detail/epilepsy# 2020.
13. Owolabi, LF, Adamu B, Jibo, AM, Owolabi, DS, Isa, AI, Alhaji, ID, et al. Prevalence of active epilepsy, lifetime epilepsy prevalence, and burden of epilepsy in sub-Saharan Africa from meta-analysis of door-to-door population-based surveys. Epilepsy Behav, 2020;103(Pt A):106846.
15. McNamara DJ. Pharmacotherapy of epilepsies. In: (Brunton, L.L., Lazo, S.J. and Parker, K.L (Eds) Gooman and Gilman’s. The pharmacological basis of therapeutics, Eleventh edition. McGraw-Hill Medical Publishing Division, New York 2006;501-525.
17. Schmidt D. The clinical impact of new antiepileptic drugs after a decade of use in epilepsy research, 2002;50:21-32.
19. Arroyo S, Brodie MJ, Aranzini G. Is refractory epilepsy preventable? Epilepsia, 2002;43:437-444.
20. Missouri Botanical Garden. Flora of China Missouri Botanical Garden Press; St. Louis http://flora.huh.harvard.edu/china/ 1994.
22. Ngassoum, MB, Ousmaila H, Ngamo, LT, Maponmetsem, PM, Jirovetz, L, Buchber G. Aroma compounds of essential oils of two varieties of the spice plant Ocimum canum Sims. from northern Cameroon J. Food Comp. Anal., 17 (2004), pp. 197-204
24. de Lima Silva L, Garlet QI, Koakoski G, de Abreu MS, Mallmann CA, Baldisserotto B, et al. Anesthetic activity of the essential oil of Ocimum americanum in Rhamdia quelen (Quoy & Gaimard, 1824) and its effects on stress parameters. Neotrop Ichthyol. 2015;13(4):715-722.
26. Usman LA, Yusuf AO, Saliu BK, Olanipekun BE. Effect of collection time on chemical composition and antibacterial activity of flower essential oil of Ocimum canum (Sims) grown in Nigeria. J Turk Chem Soc Chem. 2017;4(1):149-164.
28. Karau GM, Njagi ENM, Machocho AK, Wangai LN, Nthinga, MJ. Chemical composition and in vitro antioxidant activities of Ocimum americanum, Adv Analyt Chem. 2015;5(2): 42-49.
30. Yamada AN, Grespan R, Yamada AT, Silva EL, Saulo Euclides Silva-Filho, Marcio José D, et al. Anti-inflammatory Activity of Ocimum americanum L. Essential Oil in Experimental Model of Zymosan-Induced Arthritis. Am J Chinese Med, 2013;41(4): 913–926.
32. Barchuk O, Pryshlyak A, Shanaida M, (2021). Chemical compositions and sedative activities of the Dracocephalum moldavica L. and Ocimum americanum L. essential oils, Pharmacologyonline, 2021;2:179-187.
34. Shadia E, El-Aziz A, Omer EA, Sabra SA. Chemical composition of Ocimum americanum essential oil and its biological effects against, Agrotis ipsilon, (Lepidoptera: Noctuidae). Res J Agri Bio Sci, 2007;3(6):740-747.
36. Sofowora EA. Medicinal Plants and Traditional Medicine in Africa. 3rd Edition. Spectrum Books Limited, Ibadan Nigeria, 2008; 97-112.
38. Evans WE. Trease and Evans Pharmacognosy. 16th Edition. Saunders Publishers, London. (2009) pp. 42–229.
40. Yakubu J, Usman YB, Balami MV, Jonathan, N, Semiu, Y, Teri, DM. Phytochemical and elemental analysis of some organic solvent leaf extracts of Piliostigma thonningii (Schumach) Milne-Redhead Ewemen J Folklore Med, 2016;2(2):49-57.
42. Swinyard EA, Woodhead JH, White HS, Franklin, MR. General principles: Experimental selection, quantification and evaluation of anticonvulsant, 3rd ed. In: Levy, RH, Mattson B, Melrum JK, Dreifuss FE. (eds), Springer-Verlag Berlin Heidelberg: New York, U.S.A, 1989; 566-874.
44. Medugu AN, J. Yakubu J, Balami VM, Medugu NM, Tata FY, Marte HI et al. Anti-epileptic studies of Chamaecrista mimosoides ethanol extract. Tropical J. Nat. Prod. Res., 2020;4(5); 203 – 209.
46. Yakubu J, Sodipo, OA, Abdulrahman FI, Balami VM. Toxicity study and anticonvulsant effect of ethanol leaf extract of Piliostigma thonningii Milne-Redhead (Fabaceae). Nig J Pharmaceut Res. 2021;17(1); 63-70.
48. Ononamadu CJ, Alhassan AJ, Imam AA, Ibrahim A, Ihegboro GO, Owolarafe AT, et al. In vitro and in vivo anti-diabetic and anti-oxidant activities of methanolic leaf extracts of Ocimum canum, Casp J Int Med. 2019;10(2):162-175.
50. Amos S, Akah, P, Enwerem N, Chindo B, Hussaini I, Wambebe C, et al. Behavioral effect of Pavetta crassipes extract on rodents. Pharmacol, Biochem. Behav. 2004;77:751–759.
52. McCaskill D, Croteau R. Prospects for the bioengineering of isoprenoid biosynthesis. Adv Biochem Eng Biotechnol, 1997;55:107–146.
54. Mamta S, Jyoti S, Rajeev N, Dharmendra S, Abhishek G. Phytochemistry of Medicinal Plants. J Pharmacog Phytochem. 2013;1(6):168-182.
56. Tapas AR, Sakarkar DM, Kakde RB. Flavonoids and Nutraceuticals: A Review. Trop J Pharmaceut Res, 2008;7:1089-1099.
58. Nicholson RA, David LS, Pan RL, Liu XM. Pinostrobin from Cajanus cajan (L.) Millsp. inhibits sodium channel-activated depolarization of mouse brain synaptoneurosomes. J Fitoterapia, 2010;81:826-9.
60. Cogolludo A, Frazziano G, Briones AM, Cobeno L, Moreno L, Federica L. The dietary flavonoid quercetin activates BKCa currents in coronary arteries via production of H2O2; Role in vasodilatation. J Cardiovasc Res, 2007;73:424-31.
62. Engelborghs S, Hooge RD, Deyn PPD. Pathophysiology of epilepsy. J Acta Neurol Belg. 2000;100:201-213.
64. Clarke, ECG, Clarke, MI. Veterinary Toxicology 2nd edition. Bellaire Tindall, New York, (1977) p. 10.
66. White HS, Wolf HH, Woodhead JH, Kupferberg HJ. The National Institute of health anticonvulsant drugs development programme. Screening for efficacy In: French, J. Leppick, I.E., and Dichtes, M.A., (Eds). Antiepileptic Drug Development: Advances In Neurology, Vol.76, Lippincott-Raven Publishers, Philadelphia, 1998;29-39.
68. Padma LL. Screening for anticonvulsant and analgesics activity. Asian J. Res Pharmacol Pharmacother, 2014;3(2) (2014) 3-6.
70. Dutta A, Handique C, Lahkar M. Evaluation of Anticonvulsant potential of Bryophyllum pinnatum in experimental animals. Asian J Biomed Pharmaceut. Sci, 2015;5(41); 18-22.
72. Sayin U, Cengiz S, Altug T. Vigabatin as an anticonvulsant against pentyletetrazole seizures. Pharmacol Res. 1993;28: 325-31.
74. Rajendra S, JW. Lynch JW, Schofield, PR. The glycine receptor. Pharmacol Ther, 1997;73:121-146.
76. Ogbonnia S, VanStaden J, Jager AK, Coker HA. Anticonvulsant effect of Glyphaea brevis (Spreng) Moraches leaf extract in mice and preliminary phytochemical tests. Nig Q J Hosp Med, 2003;13(3-4):62-64.

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