Volume 5, Issue 1 (6-2023)
pbp 2023, 5(1): 93-102 |
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Yakubu J, Hassan A M, Yesufu H B, Abdulrahman F I, Sodipo O A, Ndai A M et al . Phyto-pharmacological Benefit of Methanol Whole Plant Extract of Ocimum canum Sims. in Chemically-induced Epileptic Rat Model. pbp 2023; 5 (1) :93-102
URL: http://pbp.medilam.ac.ir/article-1-176-en.html
URL: http://pbp.medilam.ac.ir/article-1-176-en.html
James Yakubu * 
1, Abdullahi Mbaya Hassan2 , Hassan Braimah Yesufu3 , Fanna Inna Abdulrahman4 , Olufunke Adebola Sodipo5 , Asinamai Medugu Ndai6 , Bitrus Wampana7
1, Abdullahi Mbaya Hassan2 , Hassan Braimah Yesufu3 , Fanna Inna Abdulrahman4 , Olufunke Adebola Sodipo5 , Asinamai Medugu Ndai6 , Bitrus Wampana7
1- Department of Pure and Applied Chemistry, Faculty of Science, University of Maiduguri, PMB, 1069, 1069, Maiduguri, Borno State, Nigeria. , jamesyakubu96@gmail.com
2- Department of Pure and Applied Chemistry, Faculty of Science, University of Maiduguri, PMB, 1069, 1069, Maiduguri, Borno State, Nigeria.
3- Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Maiduguri, PMB, 1069, Maiduguri, Borno State, Nigeria.
4- Department of Pure and Applied Chemistry, Faculty of Science, University of Maiduguri, PMB, 1069, Maiduguri, Borno State, Nigeria
5- Department of Clinical Pharmacology and Therapeutics, Faculty of Basic Clinical Sciences, College of Medical Sciences, University of Maiduguri, Borno State, Nigeria.
6- Department of Pharmaceutical Outcomes and Policy, College of Pharmacy, University of Florida, Florida, USA.
7- Department of Pharmacology, Faculty of Veterinary Medicine, University of Maiduguri, Nigeria
2- Department of Pure and Applied Chemistry, Faculty of Science, University of Maiduguri, PMB, 1069, 1069, Maiduguri, Borno State, Nigeria.
3- Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Maiduguri, PMB, 1069, Maiduguri, Borno State, Nigeria.
4- Department of Pure and Applied Chemistry, Faculty of Science, University of Maiduguri, PMB, 1069, Maiduguri, Borno State, Nigeria
5- Department of Clinical Pharmacology and Therapeutics, Faculty of Basic Clinical Sciences, College of Medical Sciences, University of Maiduguri, Borno State, Nigeria.
6- Department of Pharmaceutical Outcomes and Policy, College of Pharmacy, University of Florida, Florida, USA.
7- Department of Pharmacology, Faculty of Veterinary Medicine, University of Maiduguri, Nigeria
Abstract: (861 Views)
Objectives: The role of Ocimum canum Sim., as a medicinal agent used for the treatment and management of diseases cannot be overemphasized. The aim of the study is to screen the Phytochemical constituents and to evaluate the antiepileptic effect of methanol whole plant extract in Wistar albino rats.
Material and Methods: The whole plant part of O. canum was extracted with 85% percentage of methanol and screened for phytochemicals. An acute toxicity study was carried out using Lorke’s method and the antiepileptic activity was evaluated using pentylenetetrazole (PTZ) and strychnine in rats. One-way analysis of variance (ANOVA) was used in the analysis of the data.
Results: The phytochemical analysis revealed the presence of saponins, cardiac glycosides, flavonoids, terpenoids, and cardenolides. The intraperitoneal median lethal dose value (LD50) of O. canum in rats was 2154 mg/kg. The extract at the dose of 300 and 600 mg/kg body weight protected 40% and 80% of experimental animals against both PTZ and strychnine-induced convulsion; protected 60% and 80% of rats against death induced by strychnine; compared to 100% protection by the standard drug sodium valproate (20mg/kg).
Conclusion: The antiepileptic investigation suggests that methanol extract of whole plant of O. canum has antiepileptic activity which could be employed for the management of epileptic related conditions
Material and Methods: The whole plant part of O. canum was extracted with 85% percentage of methanol and screened for phytochemicals. An acute toxicity study was carried out using Lorke’s method and the antiepileptic activity was evaluated using pentylenetetrazole (PTZ) and strychnine in rats. One-way analysis of variance (ANOVA) was used in the analysis of the data.
Results: The phytochemical analysis revealed the presence of saponins, cardiac glycosides, flavonoids, terpenoids, and cardenolides. The intraperitoneal median lethal dose value (LD50) of O. canum in rats was 2154 mg/kg. The extract at the dose of 300 and 600 mg/kg body weight protected 40% and 80% of experimental animals against both PTZ and strychnine-induced convulsion; protected 60% and 80% of rats against death induced by strychnine; compared to 100% protection by the standard drug sodium valproate (20mg/kg).
Conclusion: The antiepileptic investigation suggests that methanol extract of whole plant of O. canum has antiepileptic activity which could be employed for the management of epileptic related conditions
Type of Study: Research |
Subject:
Pharmacognosy
Received: 2023/03/4 | Accepted: 2023/03/15 | Published: 2023/05/31
Received: 2023/03/4 | Accepted: 2023/03/15 | Published: 2023/05/31
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